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Citations for Bio-Rad :
501 - 550 of 5925 citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the sample boiled for five minutes at 95 °C and size-separated by SDS-PAGE using a 4–20% Mini-PROTEAN® TGX™ Gel (Biorad). Proteins were transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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bioRxiv - Genetics 2019Quote: ... and heated at 90°C for 5 min before being run on a stain free gel and imaged by GelDoc imager (BIO-RAD).
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
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bioRxiv - Neuroscience 2019Quote: ... and then eluted by boiling in SDS lysis buffer for 5 minutes at 95°C and resolved by SDS-PAGE (Bio-rad) gel ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Genetics 2021Quote: ... Samples were denatured at 100°C for 10 min and 5% of samples were loaded and run on a 10% Mini-Protean TGX gel (Bio-Rad). Electrophoresed proteins were transferred onto a nitrocellulose membrane using Turbo-blot Transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were boiled at 95 °C for 5 minutes and loaded to SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes (162-0112, Bio-Rad). Immunoblot analysis was performed with the following antibodies ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad). Blotting was performed onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Bioengineering 2023Quote: ... The mixture was then heated to 95 °C for 5 min and loaded into pre-cast polyacrylamide gels (Mini-PROTEAN TGX, Bio-Rad) for electrophoresis in TGS buffer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 20 μg of proteins of each sample or both input and eluates from the IPs were heated in Laemmli loading buffer at 95°C for 5 min and loaded on a SDS-polyacrylamide electrophoresis gel (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg protein per sample was heated to 95 °C for 5 min in sample loading buffer and separated by SDS-PAGE using the Mini-PROTEAN electrophoresis system (Bio-Rad). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then boiled at 95°C for 5 minutes and 20 μL of the samples were loaded into a pre-made polyacrylamide gel (Bio-Rad) immersed in 1x Tris-Glycine-SDS Buffer ...
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bioRxiv - Microbiology 2023Quote: ... Samples (24 µg) were denatured by heating 5 minutes at 95°C and separated by Stain-Free SDS-PAGE gel (4568096, Bio-Rad). The gel was activated using one-minute setting (Bio-Rad Imager ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins samples were denatured in SDS-sample buffer for 5 min at 95°C and loaded onto Mini-Protean TGXTM gels (BioRad Laboratories) in equal amounts and subjected to electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 10 min final extension at 72°C before resolving on a 5% polyacrylamide tris-borate-EDTA (TBE) gel (Bio-Rad) at 35 V ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Biochemistry 2024Quote: ... Images were collected following 5 days of incubation at 30 °C with a Bio-Rad Chemidoc MP Imaging System (Bio-Rad) and analyzed using the ImageJ software (50).
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed with IP lysis buffer three time and boiled in 25 ul of 2X SDS-loading buffer for 5 minutes and loaded into 4-15% polyacrylamide gels (BioRad).
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bioRxiv - Cell Biology 2019Quote: ... a total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Microbiology 2022Quote: ... pBAC-BoHV-4-A-revORF45HA and pBAC-BoHV-4-A-ΔORF45KanaGalK DNAs (5 μg) were electroporated in 600 μl DMEM high without serum (Biorad, Gene Pulser XCell ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed in 4 x 5 minutes in 0.2% TBS tween before incubating in Clarity™ Western ECL Substrate (BioRad) for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Immunology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 4.3 µL of cDNA was mixed with 5 µL iTaq Universal SYBR Green Supermix (BioRad 1725124) and 0.7 µL of 5 µM forward and reverse qPCR primers targeting the gene of interest (333 nM final primer concentration ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...