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Citations for Bio-Rad :
501 - 550 of 6662 citations for 1 3 Nitro 10 11 dihydro 5H dibenzo b f azepin 5 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... diluted to 1:5,000 in 1X TBST 5% milk powder for 1 hour before visualisation with Clarity™ Western ECL Substrate (Bio-Rad, Hercules, California, US) using a Syngene G ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 1:10 for all RT-qPCR experiments and SsoFast EvaGreen Mastermix (Biorad) was used to amplify cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... 1/10 000 in TBS-T) and finally revealing thanks to ECL (Biorad 170-5161) under the ChemiDoc imaging system (BioRad).
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bioRxiv - Genetics 2020Quote: ... We amplified the product in 10 μl reactions containing 1×Precision melt supermix (Bio-Rad), 200 nm of each primer ...
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bioRxiv - Biochemistry 2024Quote: ... The 10% APS solution was made by dissolving 1 g of APS (#1610700, BIO-RAD) in 10 mL water and storing at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 well 10% TBE-Urea Gel (Bio-Rad) and run for 40 minutes at 200 V ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were then run on either 4-15% (a) or 8-16% (b) precast gels (Biorad), transferred onto 0.1μm nitrocellulose membranes and used for immunoblot detection ...
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bioRxiv - Immunology 2020Quote: cDNA was generated from sorted B cells by adding 4µl of iScript (Bio-Rad Cat# 1708891) and cycling according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 2) Arm B was purified on an ENrich™ SEC 650 24 mL column (Bio-Rad), and 3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Single antigen-binding B cells were sorted using an S3 cell sorter (Bio-Rad, Hercules, CA), and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio ...
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bioRxiv - Physiology 2023Quote: ... cDNA synthesis from RNA was completed using 5x iScript RT Supermix (Bio-Rad, Catalog #L001404 B) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% BSA or 5% milk dissolved in TBS (Biorad) containing 0.1% Tween 20 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and finally TMB (3,3’, 5, 5’-tetramethylbenzidine) Core+ reagent (Bio-Rad cat # BUF062C) for development ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% (BioRad) and run for 1 hour at 150 volts ...
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bioRxiv - Genomics 2022Quote: ... 22 μL reactions were prepared with 11 μL of 2x ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad Laboratories, Inc., Cat. No. 1863023), 0.4 μM of all probes and primers ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
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bioRxiv - Genetics 2021Quote: ... Samples were then diluted 1:5 and placed into a 96 well plate with Supermix (no dUTPs, BioRad) and the required target locus primers and MGB-NFQ probes (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked 1 hour in skim milk 5% TBS-Tween buffer (TBST, Bio-Rad, 0.05% Tween 20), and incubated overnight with the primary antibodies diluted in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... then incubated with ProSignal Pico Spray chemiluminescence substrate (Prometheus) for 1-3 minutes at room temperature and imaged using a ChemiDoc Imaging System (Bio-Rad). Contrast was occasionally adjusted to improve visualization of bands ...
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bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Immunology 2024Quote: ... After final wash and supernatant removal, 1x Loading Buffer (40 μL, 3:1 Lysis Buffer:4x Laemmli Loading Buffer (Bio-Rad)) was added to the sample and the sample boiled (10 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... and plasmid copy number was quantified using quantitative PCR with qPCR-GW-F and qPCR-GW-R and SsoAdvanced Universal SYBR Green Supermix (Biorad). Each pooled sample was confirmed to contain at least 107 plasmids.
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bioRxiv - Synthetic Biology 2022Quote: ... and the plasmid copy number was quantified using quantitative PCR with qPCR-GW-F and qPCR-GW-R and SsoAdvanced Universal SYBR Green Supermix (Biorad). Each pooled sample was confirmed to contain at least 107 plasmids so that sufficient coverage of the sgRNA library is ensured.
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bioRxiv - Synthetic Biology 2024Quote: ... and the plasmid number was quantified using quantitative PCR with qPCR-GW-F and qPCR-GW-R and SsoAdvanced Universal SYBR Green Supermix (Biorad). Each pooled sample was confirmed to contain at least 107 plasmids so that sufficient coverage of the sgRNA library is ensured.
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bioRxiv - Cell Biology 2020Quote: ... and 72 for 1 minute) and 72 °C for 10 minutes in a thermocycler (Bio-Rad). Amplified products were separated on 1.0% agarose gel electrophoresis (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... surface CD8 was labeled with 10 µg ml−1 AlexaFluor647-anti-CD8 antibody (Bio-Rad, MCA1226A647) at 37 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used: goat α-mouse (1:10 000, Bio-Rad, 170-6515), donkey α-goat (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The medium was treated with 10 g·l-1 of BT Chelex 100 resin (BioRad, Hercules, CA) for 2 h and then filtered using a vacuum-driven 0.22 µm filter (Olympus plastics ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR reaction consisted of 10 μL of 1× SYBR green supermix (Bio-Rad Laboratories, Inc.), 1 μL (10 μM ...
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bioRxiv - Systems Biology 2020Quote: ... Slides were washed with PBS (3 times, 3 min) and developed using the BCIP-NBT detection system (Bio-Rad Laboratories) for 12 min followed by washing with PBS (3 times ...
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bioRxiv - Physiology 2021Quote: ... RNA was transferred onto a nylon membrane (Hybond-N; Biodyne B) using Trans-Blot Turbo (Bio-Rad) at 25 V for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were performed using a 96 well plate with an optically clear microseal ‘B’ film (Bio-Rad). Three technical replicate reactions were performed for each biological replicate ...
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bioRxiv - Cell Biology 2021Quote: ... After 3 washes with 0.05% tween20 (Bio-Rad) in TBS buffer (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... in a 1-to-1 ratio and separated by gel electrophoresis in a 10% Mini-PROTEAN TGX Precast gels (Bio-Rad Watford, UK). After gel electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μL of each primer 10 μM and 1 μL of template DNA and using a MyCycler Thermal Cycler (BioRAD, Hercules, CA, USA) with the following program ...
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bioRxiv - Immunology 2020Quote: ... Protein profile was analyzed using 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described by Laemmli [11] and stained with Coomassie Brilliant Blue (Bio-Rad, Rockville Centre, NY 11571) [12] ...
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bioRxiv - Biochemistry 2022Quote: ... 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG, GE17-6001-11 Bio-Sciences AB, Bio-Rad, Hercules, CA, USA). Proteins were focused at room temperature in a Protean IEF Cell (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 µL of SybrGreen (BioRad) and 2.5 µL of primer-mix (800 nM ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...