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Citations for Bio-Rad :
451 - 500 of 6662 citations for 1 3 Nitro 10 11 dihydro 5H dibenzo b f azepin 5 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
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bioRxiv - Developmental Biology 2023Quote: ... All cDNA samples were diluted 1:5 and 1 μL was used in quantitative PCR (qPCR) reactions with iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124), according to manufacturer’s instruction and using the Applied Biosystems QuantStudio 7 flex qPCR System ...
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bioRxiv - Bioengineering 2023Quote: ... GL Sciences, Tokyo, Japan, F10-G-10, 10 × 10 mm2; F10-G-20, 20 × 10 mm2, a polystyrene cuvette: BIO-RAD, Hercules, CA, #1702415, 10 × 10 mm2), and was placed on a suspended sample stage combined with a θ stage (Thorlabs ...
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bioRxiv - Cell Biology 2020Quote: ... X-ray film (Figs 1-5) and the ChemiDoc MP Imaging System (Bio-Rad, cat#17001402) (Supplemental Fig 3).
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bioRxiv - Microbiology 2021Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... and applied to a 5 mL prepacked Mini ceramic hydroxyapatite (CHT) type 1 column (Bio-Rad). The flowthrough was collected and purified by SEC using a HiLoad 16/600 Superdex 200 (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with Tidyblot (diluted 1:5000 in 5% BSA in TBST, Biorad, USA), for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad, France), 4 μl of cDNA diluted 1:15 in DEPC-water and 0.5 μl of each forward and reverse primers at a final concentration of 0.5 μM (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95° and resolved on 10 % or 7.5 % Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033, 4561036, 4561023, 4561026). The SDS-PAGE was performed at 100 V for 5 minutes and then 120 V for 1 hour 15 minutes using SDS running buffer (30 g Tris ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Microbiology 2020Quote: ... 22 μL reactions contained 11 μL of 2x ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad Laboratories, Inc., Cat. No. 1863023), 0.4 μM of all probes and primers ...
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bioRxiv - Microbiology 2022Quote: ... rabbit polyclonal anti-nsP1 (both validated in our laboratory) [11] and mouse monoclonal anti-GAPDH (Cat. # VMA00046, Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... EV-derived cDNA samples (5.5 μl each) were mixed with 11 μl of 2X QX200™ ddPCR™ EvaGreen Supermix (Bio-Rad) and 5.5 μl of primers (35 nM each) ...
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bioRxiv - Microbiology 2024Quote: ... and gyrA (control) gene-specific qPCR F/R primers in a 1X SYBR green master mix (Bio-Rad). Threshold and baseline parameters were kept consistent for experiments performed on different days ...
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bioRxiv - Molecular Biology 2021Quote: ... The products were separated on native 10% polyacrylamide gels (acrylamide: bisacrylamide 19:1, Biorad). The gels were then dried and exposed to storage phosphor screens (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... A 10 µl reaction was composed of 1× SYBR Green Supermix (Bio-Rad, US), 0.4 µM forward and reverse primers and 1 µl of cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 hour at 37 °C before resolved on 10% TBE PAGE gels (BioRad) run at 100 volts for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... at 10 V for 1 hr using Trans-Blot Turbo Transfer System (Bio-Rad). Then ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cDNA was diluted 1:10 and used for qPCR with SsoFast EvaGreen Supermix (BioRad). qPCR reactions were done in triplicate and normalized against Gapdh (Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1 μg was boiled for 10 min in 1x Laemmli Sample Buffer (BioRad) with 5% 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... expression media samples were diluted with 2X reducing SDS sample buffer at 1:1 ratio and the mixture was loaded on a 10-well 4–20% SDS-PAGE gradient gel (BioRad). Image J software was used to quantify the signal compared to the negative control (mock transfected culture) ...
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bioRxiv - Cell Biology 2022Quote: ... before equal volumes of substrates A and B of Enhanced Chemiluminescence (ECL) System (BioRad, UK) were added on top of the membrane to cover it and allowed to act on the antibodies for 30 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... The plates were sealed with optical-quality sealing film (Microseal® B Seals, BIO-RAD) and centrifuged at 2,000×g for 30 s ...
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A LRRK2/dLRRK-mediated lysosomal pathway that contributes to glial cell death and DA neuron survivalbioRxiv - Neuroscience 2021Quote: ... according to the manufacturer’s instruction (Magic Red® Cathepsin B assay kits, Cat. #ICT937, BioRad). The red fluorescent intensities representing cathepsin B activity in the cells were monitored under the Nikon A1 microscope.
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bioRxiv - Microbiology 2023Quote: ... and sealed with adhesive Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad Cat. No. MSB1001). Each biological replicate was analyzed in duplicate together with no reverse transcriptase and no template controls ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated shaking for 1 hour at RT with 4% blocking solution (5% milk powder (BioRad) in TBS-T) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... 250 μL of the mixed cell suspension was chilled on ice for 5-10 minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell mixture was electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 250 μL of this cell suspension and 1000 ng of a vector plasmid digested with XbaI and KpnI enzymes was chilled on ice for 5–10 minutes within a 4-mm width cuvette compatible with the Gene Pulser®/MicroPulser™ system (BioRad, Hercules, CA). The cell-DNA mixture was then electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Physiology 2023Quote: ... 18 and 21 months of age and were subjected to IEF using a 24 cm immobiline DryStrip (pH 6-11) (BIO-RAD, Hercules, CA), followed by SDS-PAGE using pre-casted 10-16% gradient polyacrylamide gels (BIO-RAD ...
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were blocked with 3% donkey serum and incubated overnight with rat anti-CD68 (1:200, Bio-Rad). Aβ deposition and CD68 staining were both developed with a Vectastain ABC kit and DAB reaction ...
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bioRxiv - Biochemistry 2023Quote: For VP protein analysis 3×109-1×1010 vg of purified AAV were mixed with 12.5 µl 4x Laemmli Sample Buffer (Bio-Rad, supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were then exposed to HRP-conjugated secondary antibody for 1 hour (1:10 000 Goat anti-rabbit IgG HRP conjugate; Bio-Rad). Antibody-protein complexes were visualized using enhanced chemiluminescence detection reagents (Clarity Western ECL Substrate ...
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bioRxiv - Developmental Biology 2023Quote: FLICA™ 660 Caspase-3/7 (BIORAD) was used to measure apoptosis according to manufacturers.
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bioRxiv - Neuroscience 2024Quote: ... and embedded in 3% agar (Bio-Rad). Sections were cut at 60 µm on a vibratome and mounted on slides ...