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Citations for Bio-Rad :
5301 - 5350 of 6638 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Physiology 2021Quote: ... cDNA was diluted 1:5 and then 2μl was added to 5ul SYBR green PCR mixture (BioRad, 1725271, Hercules, CA, USA), 2.4ul water and 1.25 pmol primer mix ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed in PBS-T for three times 5 min and developed through chemiluminescence using a ChemiDoc XRS (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... or 400 ng of total RNA was reverse transcribed to cDNA in a 20 µL reverse transcription reaction using 5 µL of iScript reverse transcription supermix (1708841, Bio-Rad Laboratories - Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature and imaged directly using a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed 3x for 5 min each in TBS without Tween and incubated with goat anti-mouse IgG coupled to horseradish peroxidase (BioRad, #170656) at a 1:3000 dilution in blocking buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were incubated with Pierce Super Signal or Millipore Immobilon enhanced chemiluminescence reagents for 5 min and visualised using a ChemiDoc system (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using the ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... in TBST/5% wt/vol non-fat milk (1 h, RT), washed (5x, TBST) followed by addition of ECL substrate (Bio-Rad) and imaged (ChemiDoc ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... were incubated in TBS-T/milk for 45 min and washed 3 × 5 min with TBS-T before detecting using a Chemidoc imager (Bio-Rad). NearIR-conjugated secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation in the substrate working solution for 5 min without shaking and exposure using ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... The membrane was incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies in 5% milk and bands were developed with Chemi-Doc XRS imaging (Bio-Rad). The primary antibodies used are listed in Supplementary Table 3.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were boiled at 95 °C for 5 minutes and loaded to SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes (162-0112, Bio-Rad). Immunoblot analysis was performed with the following antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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bioRxiv - Cell Biology 2022Quote: ... on ice for 24–36 h and then removed through glutathione sepharose 4B beads (5 mL bed volume) packed twice in Poly-Prep® Chromatography Columns (BioRad). UtrCH-sfGFP and LifeAct-sfGFP were further purified through gel filtration column chromatography (HiLoad 16/600 Superdex 75 pg ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg protein per sample was boiled for 5 min in sample loading buffer and separated by SDS-PAGE using the Mini-PROTEAN electrophoresis system (Bio-Rad). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked by incubating in 15 mL of blocking solution (5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:5 in deionized water and used as the template for quantitative PCR with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). For relative quantification ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ // Reverse: 5’-CGA AGG TGT GAC TTC CAT G-3’) and CFX96 Touch Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred onto Immun-Blot PVDF membrane (BioRab, 1620177) and incubated overnight in blocking solution (5% powdered milk, NaF in TBST [TBS (BioRad, 1706435) 1% Tween] ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-amplified cDNA was diluted at least 5-fold with nuclease-free water and mixed with SsoFast EvaGreen with Low ROX (BioRad #1725211) and chip-specific DNA Sample Reagents before loading into primed Flex Six or 96.96 Dynamic Array chips (Fluidigm #100-6308 ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were washed in 1X PBS with 0.1% Tween 3 times for 5 minutes each before scanning using the ChemiDoc™ MP Imaging System (Bio-Rad). Note ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were washed again in PBS-Tween before being placed in 0.1 mL working solution (equal parts stable peroxide solution and Luminol/Enhancer solution) per cm2 membrane for 5 minutes before being placed for imaging in ChemiDoc Gel Imaging System (Bio-Rad). Gels were quantified using image J by taking the ratio of the integrated intensity (area x intensity ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fractions containing Chimera were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were washed three times in TBST for 5 minutes each and incubated with an anti-mouse HRP secondary (Bio-Rad) at 1:2000 dilution for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membranes were blocked for 1h at RT in 5% Blotting Grade Blocker Non-Fat Dry Milk (Biorad, Hercules, CA, USA) and TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of synthesized cDNA was firstly diluted in a 1:5 ratio and used in a 20 µL reaction volume using the CFX96 Touch Real-Time PCR Detection System (BioRad, USA) with the following thermal cycling conditions ...
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bioRxiv - Biochemistry 2024Quote: ... Images were collected following 5 days of incubation at 30 °C with a Bio-Rad Chemidoc MP Imaging System (Bio-Rad) and analyzed using the ImageJ software (50).
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2024Quote: ... equipped with a 5-mL Foresight Nuvia HPQ anion-exchange column prepacked with CHT ceramic hydroxyapatite XT mixed-mode chromatography media (Bio-Rad) or 25-mL column in-house packed with the same CHT media ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then washed five times with TBST and incubated for 5 min with ECL substrate (Clarity Western ECL substrate, Bio-Rad) in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... they were heated at 95 degrees for 5 minutes after addition of TBE Urea buffer (Novex) and immediately run on a 15% TBE-Urea gel (Bio-Rad). The gels were then imaged using Cy2 filter on ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The immunoblot was washed three times for 5 min each in TBST buffer and developed using clarity Western ECL substrate (Bio-Rad). Finally ...
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bioRxiv - Developmental Biology 2022Quote: Protein extracts were separated on a 12% SDS gel with a 5% stacking gel and transferred to PVDF membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked using 5% milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...