Labshake search
Citations for Bio-Rad :
5551 - 5600 of 6638 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... 10 μg of total protein for both NT and Rab siRNA-transfected samples were resolved in 4-20% gradient SDS-PAGE (Mini-PROTEAN Gel, Cat. 4561094, Bio-Rad Laboratories, Hercules, CA) and subsequently transferred to a PVDF membrane for immunoblotting (Immun-Blot PVDF Membrane ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were boiled in Laemmli sample buffer before separation by SDS-PAGE on 4-20% TGX™ precast gels (Bio-Rad, Hercules, CA, USA). Gels were transferred to nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Genomics 2024Quote: ... The multiple detection of the four known DENV serotypes (DENV-1 to 4) was performed in a CFX Opus Real-Time PCR System (Bio-Rad, Hercules, CA, USA).
-
bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
-
bioRxiv - Biophysics 2023Quote: ... the protein concentrations were determined by image analysis of SDS-PAGE with 4-20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad, 4568096) followed by Coomassie Brilliant Blue staining using the following concentration standard ...
-
bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
-
bioRxiv - Physiology 2023Quote: ... 5 μg of protein homogenate was separated on Criterion™ XT Bis-Tris (RyR, SERCA, Actin) or 4–15% Mini-PROTEAN® TGX™ Precast stain-free (CaMKIIδ) gels (Bio-rad) and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 25ug of protein was used for Western blot run in 4-20% gradient gel (Criterion TGX Stain-free, #5678094, Bio-Rad, Hercules, California, USA). Proteins were transferred to nitrocellulose membrane in Turboblotter (Bio-Rad ...
-
bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
-
bioRxiv - Genetics 2023Quote: ... The purity of viral preparations was determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis on a 4– 15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Hercules, CA, USA) in Tris-glycine- SDS buffer (Figure S3).
-
bioRxiv - Bioengineering 2023Quote: ... was subjected to SDS-PAGE using a 4-20% Mini-PROTEAN TGX acrylamide gel on a Mini-PROTEAN Electrophoresis cell (Bio-Rad Laboratories, CA, USA). Electrophoretically separated proteins were transferred to Immobilon-P PVDF membrane (25V/RT/overnight) ...
-
bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2024Quote: ... and subsequently subjected to SDS-polyacrylamide gel electrophoresis (PAGE) on a 4–15% Mini Protean TGX precast gel (Bio-Rad Laboratories, Hercules, CA, USA).
-
bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... 10 µg of each lysate sample were separated though sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Criterion precast gels (Bio-Rad Cat #456-1095). Proteins were blotted onto Polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2024Quote: ... was chilled on ice for five to ten minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell-DNA mixture was electroporated using the GenePulser XCell™ device (BioRad ...
-
bioRxiv - Neuroscience 2024Quote: ... and samples were boiled for 5min at 95°C and then loaded on 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad #4568093). Gel was run at 100V for 90 minutes and proteins were transferred to a nitrocellulose membrane using the Bio-Rad Transblot Turbo system ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples containing 15 µg of total proteins were run in duplicate onto 4-15% Criterion™ TGX Stain-free™ precast gels or 12% Criterion™ TGX precast gels (Bio-Rad) and transferred into nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Physiology 2024Quote: Thirty micrograms of protein from the cell lysate in RIPA buffer was separated by reducing SDS-PAGE on a 4-15% polyacrylamide gel (Bio-Rad, Hercules, California, USA). The protein was transferred to a nitrocellulose membrane using a semi-dry transfer method [36] ...
-
bioRxiv - Genomics 2024Quote: ... CD45-positive and CD3-positive cells were centrifuged at 400 x g for 7 minutes at 4 ºC and counted with a TC20™ Automated Cell Counter (Bio-Rad Laboratories, S.A), before proceeding to single cell RNA sequencing.
-
bioRxiv - Biophysics 2024Quote: ... before boiling the sampling in a reducing sample buffer and separation on a 4-15 % mini-PROTEAN Precast SDS-PAGE (Bio-Rad, Cat No 4561085). Transfer to PVDF membrane was performed using a Trans-Blot pack (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 15ug of denatured protein lysate was separated on a 4-15% Mini-Protean TGX Precast Protein Gel (Cat# 4561086, Bio-Rad Laboratories, Inc. USA). After transfer to 0.2 um PVDF membrane using TransBlot Turbo Transfer system (Cat# 1704272 ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 16,100 rcf for 15 minutes at 4 °C and protein concentration was quantified using the DC™ Protein Assay kit (Bio-Rad, Hercules, CA).
-
bioRxiv - Biochemistry 2024Quote: Cell lysates containing 1X SDS buffer were homogenized with 27-G syringe and whole lysates were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels before being transferred to PVDF membranes (Bio-Rad ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
-
bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... for 5 minutes and then transferred onto a protran BA-85 nitrocellulose membrane using a semi-dry transfer apparatus (Biorad 170-3940) for 75min at 400mA (not exceeding 15V) ...
-
bioRxiv - Molecular Biology 2021Quote: 5 μg of cheRNA or total RNA for each condition were denatured at 95°C for 5 min and transferred to a Hybond-XL membrane (Amersham) in a Bio-Dot® Microfiltration Apparatus (Bio-Rad) following manufacturer’s intructions ...
-
The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 10μl of EMSA binding reactions were loaded on 5% nondenaturing polyacrylamide Mini-PROTEAN TBE gels (Catalog #4565015, Bio-Rad Laboratories, Hercules, CA) for electrophoretic analyses ...
-
bioRxiv - Biochemistry 2020Quote: ... protein was eluted from the beads by boiling for 5 minutes at 95°C in Laemmli Sample Buffer (BioRad, catalog #161-0747). Proteins were stored at −80°C until western blotting.
-
bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
-
bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
-
bioRxiv - Microbiology 2021Quote: ... and 5 µl of PowerUp™ SYBR™ Green Master Mix (2X) using a CFX Connected Real-Time PCR machine (Bio-Rad). Primers used in this study were listed in Table S3 ...
-
bioRxiv - Biophysics 2020Quote: ... the RNC samples from the above sucrose cushions were heated at 55 °C for 5 min in the presence of 1X Laemmli Sample buffer (Bio-Rad, 1610737). Subsequently ...
-
bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
-
bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
-
bioRxiv - Neuroscience 2022Quote: ... Blots were blocked for 1 h at room temperature (RT) in Tris-buffered saline with 0.05 % Tween-20 (TBST) containing 5 % blotting-grade dry milk (Biorad cat# 170-6404), incubated overnight with primary antibody (dilutions above mentioned ...
-
bioRxiv - Immunology 2020Quote: ... Proteins were denaturated at 95°C for 5 minutes before separation on SDS-PAGE TGX 12% Stain-Free FastCast Acrylamide (Bio-Rad Laboratories). Proteins were then electro-transferred onto Trans Blot Turbo polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
-
ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... and the corresponding 5 μl of Primer Master-mix was added followed by sealing the plate with Microseal ® adhesive sealer (Bio-Rad). The 60S acidic ribosomal protein P0 (rplp0) ...
-
bioRxiv - Genomics 2020Quote: ... 5 µL of cDNA was pre-amplified in a 12-cycle PCR reaction using the Sso Advanced PreAmp Supermix (Bio-Rad, USA) in a 50 µL reaction ...
-
bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
-
bioRxiv - Microbiology 2022Quote: ... burgdorferi 16S rRNA by preparing samples in a 96-well plate using 5 µL of 2X SYBR Green Master Mix (Bio-Rad #1725121), 1 µL of each primer (Forward ...
-
bioRxiv - Bioengineering 2022Quote: ... that consists of 1 mL peroxide and 1mL luminol enhancer were spread onto membrane and left for 5 minutes before observation using ChemiDoc imager (Bio-rad Laboratories).
-
bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
-
bioRxiv - Biochemistry 2021Quote: ... Membranes were incubated with TBST-Blotto buffer (50 mM Tris-pH 7.5, 150 mM NaCl, 0.1% Tween-20, 5% w/v Bio-Rad Blotting Grade Blocker) for 1 h at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...