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Citations for Bio-Rad :
5001 - 5050 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the proteins were separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, USA). The blots were blocked in 5% BSA in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Equal volumes of sample were separated on 4–12% or 10% Criterion XT Bis-Tris Precast gels (Bio-Rad, Hercules, CA, USA) using XT MES Running buffer (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts (10 µg) of nuclear proteins from different samples were separated using SDS– PAGE and electrotransferred to a nitrocellulose membrane (BioRad, 162-0115) for 1.5 h at constant 400 mA at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% Silwet L-77 (Lehle Seeds Inc) solution followed by incubation in the dark for 10 min and imaging using a ChemiDoc™ Touch System (Bio-Rad). Alternatively ...
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bioRxiv - Neuroscience 2022Quote: ... while brain cytokine analyses for Studies C and D were conducted using a custom 10-plex Bioplex (Bio-rad Laboratories, Hercules, CA) as previously described (Finnell ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (∼15 μg) were loaded and separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Hercules, CA), and transferred to polyvinilidene difluoride membranes (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Laemmli buffer was added to samples.10 μL of samples were loaded onto 12 % Mini-P ROTEAN® TGX™ Precast Protein Gels (Biorad). Silver staining was carried out at room temperature for 1 h and gels were developed for 2 to 3 mins ...
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bioRxiv - Bioengineering 2022Quote: ... were separated on a 4-10% SDS-polyacrylamide gel at 120 V for two hours using a PowerPac Basic setup (BioRad Laboratories Inc.). The proteins were transferred onto a 0.45 µm nitrocellulose membrane at 75 V for 90 min using a transfer assembly (BioRad Laboratories Inc.) ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of 10-fold diluted cDNA was combined with 5 µL of iQ SYBR Green Supermix (Bio-Rad, Cat# 170-8880) and 0.5 µL of each 10 µM forward and reverse primers ...
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bioRxiv - Neuroscience 2024Quote: ... were resolved by SDS-PAGE on 4-15% Mini-PROTEAN TGX Stain Free gels (30 μL, 10 well comb, BioRad, Cat# 4568083) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The libraries were then diluted to 10 nM and further quantitated by qPCR on a CFX Connect Real-Time qPCR system (Biorad, Hercules, CA) for accurate pooling of barcoded libraries and maximization of the number of clusters in the flow cell.
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bioRxiv - Molecular Biology 2024Quote: ... The remaining 30 μL sample was run on a 10% acrylamide SDS-PAGE gel at 100 V for 15 minutes and stained with Coomassie (Bio-Rad #P1610786) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036), and electrophoresis was performed according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was performed using 10 μg of NP-40 extract protein mixed with an equal volume of 2X Laemmli buffer (BioRad cat#: 1610737) supplemented with 5% 2-mercaptoethanol (without boiling) ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Bioengineering 2024Quote: Protein expression was confirmed by SDS-PAGE analysis using 10% precast gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Cat# 4561033). The gels were run for 55-minutes at 150V and stained with G-250 Coomassie stain (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 µg were then loaded and separated on precast 4–20% polyacrylamide Mini-PROTEAN TGX gels (Bio-Rad Laboratories, Hercules, CA). The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Equal amounts (10 μL) of the eluted solution from the streptavidin pulldown were loaded in a 4-20% polyacrylamide gel (Bio-RAD 4561096) and separated with SDS-PAGE at 100 V for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: The histones containing residue-specific acetylations or the nucleosomes used for the acetyltransferase activity assay were electrophoresed in a 10–20% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) (DRC, NXV-396HP20) and transferred onto a nitrocellulose membrane (BIO-RAD, 1620112) at 20 V for 10 min by the semi-dry method ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves were kept in the dark for 10 min before luminescence was detected with a ChemiDoc XRS+ imaging system (BioRad, Feldkirchen, Germany). Luminescence intensities mm-2 were evaluated using the Image Lab software (BioRad ...
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bioRxiv - Genetics 2023Quote: ... Expression was measured with 20 ng of cDNA (calculated based on starting RNA quantity) in 10 μL reactions with SYBR Green (Bio-Rad, 1725124). Relative expression for knock-down was calculated using the Delta-Delta Ct method with Gapdh as the control reference ...
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bioRxiv - Cancer Biology 2023Quote: ... Between 40mg and 120mg of each protein sample was boiled in Laemmli buffer with 10% β-Mercaptoethanol and equal percentages of each sample were run on 4-15% polyacrylamide gels (BioRad, 4561086). Resolved proteins were wet-transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of cDNA (10 ng of starting RNA) was amplified per reaction using the iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124) and the Bio-Rad CFX96 qPCR system.
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bioRxiv - Biochemistry 2023Quote: ... 14 μL of Novex Hi-Density TBE Sample Buffer was added to the samples and separated in a 10% polyacrylamide non-denaturing gel (Mini-PROTEAN TGX, BIO-RAD #4561023) using 1X TBE running buffer for 30-35 min at 200 V ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of protein from each lysate were resolved on 10% or 12% sodium dodecyl sulphate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... adjusted to equal concentration for each sample and boiled at 95°C for 10 minutes in Laemmli buffer (Bio-Rad #161-0747).
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bioRxiv - Cancer Biology 2022Quote: ... The beads were washed three times with 1X PBST and resuspended in 1X Laemmli sample buffer 10% BME (Bio-Rad 161-0747).
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bioRxiv - Evolutionary Biology 2023Quote: ... purified protein fractions were separated with 10%–20% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotter (ATTO ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein was diluted with Laemmli buffer and loaded without heat denaturation onto a Criterion 10-20% tris-HCl gel (Bio-Rad, 3450043) run in tris-glycine SDS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... were incubated in a boiling bath for 10 min and analyzed by SDS-PAGE on 4–20% precast polyacrylamide gradient gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using antibodies against MBP (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were loaded on a 10% SDS-PAGE gel made in-lab or on a 4-15% pre-cast TGX gel (Bio-Rad, #4568086), along with the molecular marker ...
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bioRxiv - Genomics 2023Quote: ... An aliquot of 500 μl was solidified on ice for approximately 10 min in a pre-cooled plug casting mold (BioRad Laboratories, Switzerland). Agarose plugs were incubated in 15 ml Falcon tubes containing 5 ml of a lysing solution containing 0.25 M EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... Migration of 20 μg of lysate supernatants was performed in 4-12 % gradient or in 10 % SDS-PAGE gels (Bio-Rad Laboratories), transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein samples (15 μg/lane) were subjected to analysis by 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620264) using either semi-dry or wet electroblotting (for proteins with a molecular weight of 100 kDa and above) ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, USA) using a semidry electrophoretic apparatus ...
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bioRxiv - Immunology 2023Quote: ... a total of 10–20 µg protein was prepared and loaded onto 4 to 20% gradient Mini-PROTEAN TGX Gels (Bio-Rad, 4561093) and electrophoretically separated at 135 V ...
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bioRxiv - Microbiology 2023Quote: ... Gels were loaded with samples containing 10 μg total protein before electrophoresis on a 16.5% Tris Tricine gel (Bio-Rad, Hercules, CA). Gels were stained with the Pierce Silver Stain kit (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: ... Absorbance was measured at 412 nm at 0 min and 10 min intervals for 4 h using a microplate reader (iMarkTM Microplate Absorbance Reader, Bio-Rad, Japan), and AChE activity was calculated as % inhibition ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then boiled in 1x Laemmli buffer with 10% β-mercaptoethanol and loaded onto 12% gradient Tris-Glycine gels (Bio-Rad). Western blotting was performed using the antibodies listed in the antibody table ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad; item # 1725211) diluted 1:2 ...
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bioRxiv - Bioengineering 2024Quote: ... The transfer to polyvinylidene difluoride (PVDF) membranes was performed with a Trans-Blot® TurboTM system (at 25 V for 10 min, Bio-Rad). Blocking was conducted for 2 h using Tris-buffered saline (20 mM Tris ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Protein extracts were boiled for 10 minutes and then loaded on polyacrylamide gels (TGX Stain-Free FastCast gels (Bio-Rad, 1610181/1610183) or home-made gels ...
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bioRxiv - Microbiology 2024Quote: ... 20 μg of total protein was loaded onto each lane of SDS-PAGE on a 10% acrylamide gel (BIO-RAD cat. #4561034) and run 45 min at 60 V followed by 45 min at 110 V ...
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bioRxiv - Plant Biology 2024Quote: ... Absorbances were measured at 412 nm at 0 min and 10 min intervals for 4 h using a microplate reader (iMarkTM Microplate Absorbance Reader, Bio-Rad, Japan) (Tables S4 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... sheep polyclonal to TGN46 (Biorad #AHP500G, 1:1000), rat monoclonal to myc-tag (Chromotek #9e1 ...