Labshake search
Citations for Bio-Rad :
4951 - 5000 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... followed by 40 cycles of denaturation at 95°C for 10 seconds and extension at 60°C for 30 seconds on the Bio-Rad CFX-96 qPCR machine (Bio-Rad, UK). Data were analysed with CFX-manager software v.3.1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
-
bioRxiv - Zoology 2019Quote: ... followed by extension at 72°C for 10 min in a T100™ Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). PCR products were run against a Genescan™ 500 LIZ™ internal size standard on an ABI 3130 Genetic Analyzer (Applied Biosystems ...
-
bioRxiv - Synthetic Biology 2019Quote: ... then the supernatants were filtered through 0.2 micron filters and run with 10 mN H2SO4 mobile phase at 0.6 mL/min on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
-
bioRxiv - Bioengineering 2021Quote: ... The resulting gene fragment was ligated with the plasmid backbone using a ligase-free ligation method developed in the lab that includes incubation with 10 mM MgCl2 in a thermocycling step in a PCR thermocycler (Biorad, T-100). The cycling programme was as follows ...
-
bioRxiv - Bioengineering 2021Quote: ... Produced protein was sequentially purified by affinity chromatography using Ni-NTA agarose beads and fast protein liquid chromatography (FPLC, NGC Quest 10 Chromatography system, Biorad, Hercules, CA). Protein fractions detected at λ = 280 were collected ...
-
bioRxiv - Bioengineering 2021Quote: ... each wash was 10-min long) and developed using the Clarity Western ECL substrate (Bio-Rad, Hercules, CA, USA, cat. #170-5060). Immunoreactive bands were visualized with ChemiDoc™ XRS Imaging System (Bio-Rad ...
-
bioRxiv - Bioengineering 2020Quote: ... Samples were denatured by incubating at 95°C for 10 minutes and loaded onto a denaturing 10% polyacrylamide gel with 8 M Urea in a Mini-PROTEAN® Electrophoresis System (BIO-RAD). Electrophoresis was performed at 240 V for 45 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
-
bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
-
bioRxiv - Neuroscience 2021Quote: A 30 μL aliquot of either medium or lysate for each sample was heated at 95 °C for 10 min and loaded on two separate SDS-PAGE gels (Any kD™ Mini-PROTEAN® TGX Stain-Free Protein Gels; BioRad). Proteins were electrophoretically transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The treated samples were subjected to SDS-polyacrylamide gel electrophoresis on 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The separated protein samples were then transferred to nitrocellulose membranes ...
-
bioRxiv - Molecular Biology 2019Quote: The optimized final reaction conditions were performed in a final volume of 10 µL containing 1X of SsoAdvanced™ Universal Probes Supermix (Bio-Rad), 575 nM of primers ...
-
bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
-
bioRxiv - Cell Biology 2021Quote: ... Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
-
bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were analyzed on 10% Tris-Tricine SDS-PAGE system (38) with Precision plus protein standard (Bio-Rad 1610363) as a molecular weight marker ...
-
bioRxiv - Developmental Biology 2022Quote: ... was added to 20 µg protein and samples were boiled at 95°C for 5 minutes before being loaded onto a 10% SDS-polyacrylamide gel (Bio-Rad #4568034). Protein was transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Plant Biology 2022Quote: ... Blots were washed with TBST buffer three times for 10 min each time and inserted between acetate transparency sheets and imaged on a ChemiDoc Touch Imaging System (Bio-Rad Laboratories) using Immobilon western chemiluminescent HRP substrate (MilliporeSigma ...
-
bioRxiv - Neuroscience 2022Quote: ... and heated at 50 °C for 30 min prior to loading on a 10% criterion Tri-HCl gel (Bio-Rad, Hercules, CA). Samples were then transferred onto PVDF membranes (Millipore Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... RPE protein homogenates (at least 10 μg/well) were separated on a tris/glycine/SDS 7.5% gradient mini-protean gel (Bio-Rad, 456-1026) and electrophoretically transferred to a nitrocellulose membrane (Bio-Rad ...
-
bioRxiv - Neuroscience 2019Quote: ... 1 ml Tween20 add to 1000 ml with ddH2O) 3 times for 20 min and blocked with 10% dried milk-TBST solution or BlockAce-TBST solution (BlockAce, Bio-Rad, #170223) for 2 h ...
-
bioRxiv - Plant Biology 2021Quote: ... Samples of about 120-160 μg were loaded into wells of a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad Laboratories) and run for 2.5 h at 90 V in buffer consisting of 25 mM Tris ...
-
bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then boiled at 95°C for 10 mins and resolved in 4-15 % Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) for immunoblot analysis ...
-
bioRxiv - Neuroscience 2021Quote: ... 40μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Membrane was blocked with 5% BSA for 60 min at room temperature on shaker ...
-
bioRxiv - Bioengineering 2021Quote: ... 10 µg/lane of S was run on 4–20% Mini- PROTEAN® TGX Stain-Free™ Protein Gels (BioRad, Cat#4568081), transferred to nitrocellulose (Sigma ...
-
bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The samples were run with 10 mM sulfuric acid at 0.6 mL/min flow rate on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 250-1000 ng of DNA was mixed with 10 µl of 2X ddPCR Supermix for Probes (no dUTP, Bio-rad; Cat#1863024), 600 nM of primers and 200 nM of probes ...
-
Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... The gel was stained with ethidium bromide (1.25 µg/mL in TBE) for 30 min and destained for 10 min before imaging on a ChemiDoc™ Touch imaging system (Bio-Rad).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each PCR reaction (10 μL final volume) contained 5 μL SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA), 100 ng of cDNA ...
-
bioRxiv - Microbiology 2020Quote: ... Forty μg of total proteins were separated by electrophoresis on 10% polyacrylamide gels and transferred to PVDF membranes (Bio-Rad, CA, USA). The membranes were blocked with 5% BSA in Tris-buffered saline for 1 h at room temperature ...
-
bioRxiv - Developmental Biology 2019Quote: ... using 9% SDS polyacrylamide gels and then transferred to a 0.2µM polyvinylidene fluoride (PVDF) membrane over 10 minutes (Bio-Rad Trans-Blot Turbo). Membranes were blocked with 5% skim milk in 0.05% TBS-T for 1 hour ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was separated from the resin and injected in Superdex 75 16/600 column pre-equilibrated with the washing buffer and eluted fractions were monitored using an NGC Quest™ 10 Plus Chromatography System (Bio-Rad). The fractions were collected and concentrated to a final concentration of 700 µM using Vivaspin sample concentrators (GE Healthcare ...
-
bioRxiv - Plant Biology 2021Quote: ... Plant leaf protein samples (30-100 µg) were run on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) and transferred to PVDF membranes ...
-
bioRxiv - Immunology 2021Quote: ... The samples were mixed by pipetting and loaded at 30 μg to a 4-20% 10-well Mini-PROTEAN TGX™ precast gel (Bio-Rad). Ten microliters of the Novex™ Sharp Pre-stained Protein standard (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... 10 μg of total cell lysates proteins were separated by electrophoresis on 10% SDS polyacrylamide gels (previously prepared following the Bio-Rad protocol) and transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins (40 μg) were loaded onto 10% Tris-glycine gel (CriterionTM XT precast gel) using XT Tricine Running Buffer (Bio-Rad, USA). After electrophoresis ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
-
bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 0.5 µL of each forward and reverse primer (10 µM) and 5 µL of SsoADV Universal SYBR® Green Supermix (Bio-Rad) were used ...
-
bioRxiv - Immunology 2021Quote: ... development buffer was added to each well and the plates were read every 10 minutes using a Model 550 Microplate Reader (Bio-Rad, UK) until the positive control reached a predetermined OD405 that was consistent across plates ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated by polyacrylamide gel electrophoresis (PAGE) on a 10% mini-PROTEAN® TGX™ precast gel (Bio-Rad, CA, USA) at 140 V and 300 mA for 1 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... prestained protein ladder were resolved on 10% acrylamide gels by SDS-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membrane (BioRad Cat. # 1620112) at 100 volts for 90 min.
-
bioRxiv - Bioengineering 2022Quote: ... Samples (10-15ug protein/lane) were run by electrophoresis on pre-cast SDS-Page Stain Free Gels (Bio-Rad, Hercules, CA, USA). Separated proteins were transferred to low fluorescence PVDF membranes using the TransBlot Turbo (Bio-Rad ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 20 ng of cDNA (based on the original RNA concentration) were used for qPCR analysis in 10 ul reactions with SYBR Green (Bio-Rad, 1725124). Expression was calculated relative to the geometric mean of the two housekeeping genes.
-
bioRxiv - Genetics 2022Quote: ... resuspended to 10 million cells/ml and sorted on a two-laser (466 nm and 561 nm) cell sorter (Bio-Rad S3) as previously described (55) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... qPCR was performed to determine the copy numbers of the EcN fimA gene in 10 ng of fecal genomic DNA using iTaq Universal SYBR Green Supermix (Bio-Rad, USA). Standard curves were constructed by quantitatively testing 108 ...