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Citations for GeneCopoeia :
51 - 93 of 93 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... All cell lines were tested for mycoplasma using MycoGuard Mycoplasma PCR Detection Kit (GeneCopoeia, Rockville, MD) every 3–6 months ...
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bioRxiv - Cell Biology 2023Quote: ... a solution was prepared by adding 2 drops of EasyProbe-Hoechst 33342 Live Cell Stain (GeneCopoeia) into 1 ml of M9 buffer ...
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bioRxiv - Immunology 2023Quote: ... . All cell lines used in this study underwent routine mycoplasma testing with MycoGuard (Genecopoeia #LT07-118). Primary human keratinocytes were derived from the foreskin of two donors of Malay ethnicity and obtained with informed consent from the Asian Skin Biobank (ASB ...
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bioRxiv - Microbiology 2021Quote: ... McLellan) with a removable C-terminal twin-strep tag was transfected into cells with EndoFectin Max (GeneCopoeia) using the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: INA-6 IL-32 KO cells were lentiviral transduced using OmicsLink™ ORF lentiviral expression system from GeneCopoeia. Vector for knock in of IL-32β ...
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bioRxiv - Cancer Biology 2021Quote: ... Stable cell lines expressing firefly luciferase were generated using lentiviral particle transduction and puromycin selection (GeneCopoeia, Rockville, MD).
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bioRxiv - Cell Biology 2020Quote: ... Cells were infected with 1 × 106 TERT Human Lentifect Purified Lentiviral Particles (GeneCopoeia, LPP-Q0450-Lv05-200-S). To aid in infection ...
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bioRxiv - Bioengineering 2020Quote: ... Viruses were produced in HEK-293Ta cells using human lentiviral packaging system according to the manufacturer’s instructions (Genecopoeia). Puromycin was used to select only for transduced cells ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were infected with lentivirus particles (MOI=20) in presence of 8 μg/ml polybrene (GeneCopoeia, Guangzhou, China) for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cotransfected with human PRNP 3’UTR miRNA Target Clone (pEZX-3’UTR-PRNP vector from Genecopoeia) and hsa-miR-519a-3p miRCURY LNA miRNA Mimic or its related Negative Control miRCURY LNA miRNA Mimic (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were co-transfected with 100ng of murine Gria1 3’ UTR renilla/luciferase plasmid (MmiT076857-MT06-GC, GeneCopoeia) or miRNA 3’ UTR target control plasmid (CmiT000001-MT06-GC ...
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bioRxiv - Bioengineering 2020Quote: ... Human lung squamous cell carcinoma dual-labeled (eGFP, Luciferase) stable NCI-H1299 (SCL-C01-HLG; Genecopoeia, Inc, Rockville, MD) cells were grown in RPMI-1640 containing 10% fetal bovine serum without antibiotics at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: Lenti-SARS-CoV-2 Full Length Spike protein-pseudotyped (WT) and ACE2+ 293 cell lines were purchased from Genecopoeia. Viruses were produced as recommended by the manufacturer and harbored a luciferase expressing plasmid ...
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bioRxiv - Physiology 2023Quote: Huh-7 or AML12 cells (2.5 x 105) were reverse transfected in triplicate in 6 well plates using Endofectin (Genecopoeia) with the indicated doses of miRIDIAN miRNA mimics (miR-541-3p) ...
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bioRxiv - Cancer Biology 2023Quote: ... MT-5 cell line were also transduced to stably express luciferase using LentifectTM (GeneCopoeia #LPP-HLUC-Lv105-025-C) lentiviral vectors of firefly luciferase ...
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bioRxiv - Cancer Biology 2020Quote: ... LNCaP-C4-2 cells were transduced to express ZsGreen or Firefly luciferase-mCherry by the Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) Cell Engineering Shared Resource using a lentivirus (Clontech or Genecopoeia). LNCaP-C4-2 and PC3-mm were sorted into CD117+ and negative populations using the Miltenyi MACS sorting beads or the ThermoFisher MagniSort CD117 (c-kit ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were transfected with an all-in-one CRISPR/Cas9 plasmid for Rab7A (plasmid number HTN218819, Genecopoeia, Rockville, MD). 72 hrs post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... lentivirus particles were made using HEK293 cells (ATCC) transfected with 2nd generation packaging/envelope plasmids (Dr. Yasuhiro Ikeda, Mayo Clinic) and shRNA clones (GeneCopoeia). U251 cells were obtained from American Type Cell Culture and maintained for in vitro experiments in DMEM supplemented with 10% FBS and 1% Antibiotic Antimycotic Solution ...
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bioRxiv - Cancer Biology 2019Quote: CRYM expressing cells were generated by transfecting PCa cells with pReceiver-M72 Empty Vector and pReceiver-M72 CRYM (for simplicity referred to in the text as CRYM(+) (GeneCopoeia) containing a green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2020Quote: ... To upregulate NMDAR expression in a cell-type-specific manner, mouse GluN1 cDNA (OMu21895D, Genescript) and mouse GluN2B cDNA (EX-Mm24581-M02, Genecopoeia) were cloned to custom-made vectors ...
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bioRxiv - Cancer Biology 2022Quote: ... Murine 266-6 cells (ATCC cat#CRL-2151) were transduced with lentivirus encoding St6gal1 (Genecopoeia, cat#LPP-EX-Mm05221-Lv105) or shRNA against St6gal1 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably expressing cloned CB2 (CB2-CHO) were produced by electroporation with the human CB2 N-3xHA tag cDNA (GeneCopoeia). A stable expressing population was selected for with 500 μg/mL G418 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then infected with SARS-CoV-2 Spike-Pseudotyped Lentivirus (Firefly Luciferase SARS-CoV-2 lentiviral particles-GeneCopoeia) and the control VSV-G protein pseudotyped Lentivirus (HLUC-Lv201 Firefly luciferase − eGFP lentifect-GeneCopoeia ...
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bioRxiv - Genomics 2021Quote: Lentivirus was produced in twelve 15cm dishes of 293T cells using Lenti-Pac HIV expression packaging kit following the manufacture’s protocol (GeneCopoeia, LT002). Lentivirus was filtered through a 0.45um PES filter system (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... MiRNA inhibition in MCF-10A cells was achieved by transducing cells with a miRNA inhibitor against hsa-miR-424-5p and hsa-miR-503-5p (Genecopoeia #HmiR-AN0494-AM03 and #HmiR-AN0550-AM03 respectively) ...
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bioRxiv - Cancer Biology 2022Quote: A375 melanoma cells were transfected with a pEZX-PG04 plasmid expressing Gaussia luciferase (GLuc) under the influence of the PARP14 promoter (GeneCopoeia). After 48 hours ...
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bioRxiv - Genomics 2022Quote: The Jurkat cell line (ATCC TIB-152) and a Cas9-stable version of Jurkat (SL555, GeneCopoeia, Inc., Rockville, MD, USA) were maintained in RPMI medium supplemented with 10% FBS at 37C under standard CO2 conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... C3H10T1/2 mesenchymal cells were transfected by the LUC-3’UTR without or with co-transfection of miR27a (MmiR3347-MR04-50, GeneCopoeia) using Lipofectamine 200 (Cat #11668027 ...
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bioRxiv - Cancer Biology 2023Quote: ... was used to generate CEACAM6 knock-out cells (A549-CEACAM6-KO) using lentivirus particles carrying the CRISPR human sgRNA for CEACAM6 (Genecopoeia). Clones were selected using puromycin and the CEACAM6-expression knockout was confirmed by flow cytometry and Western blot.
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bioRxiv - Cancer Biology 2023Quote: ... To generate stable HD-PTP knockdown cell lines we transduced SW1573 and H1299 cells with 3 individual HD-PTP shRNAs (LPP-HSH067569-LVRH1GH) and control lentiviral particles (Scramble, LPP-CSHCTR001-LVRH1GH) (GeneCopoeia). Cells were selected with hygromycin and the strongest HD-PTP shRNA knockdown was used for the remaining experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... were infected with lentiviral particles produced by transfecting 293T cells using the lentiviral expression vectors and the Lenti-PacTM HIV Expression Packaging system from Genecopoeia. The PDAC cells were selected with puromycin (4 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... were infected with lentiviral particles produced by transfecting 293T cells with the lentiviral expression vectors and the Lenti-PacTM HIV Expression Packaging system from Genecopoeia. Stable clones of the PDAC cells were obtained by selection with 4 μg/ml puromycin (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2019Quote: ... U87MG cells stably expressing C-terminal 3x FLAG tagged DDX28 were generated by transfecting cells with the OmicsLink™ pEZ-M14 EX-A3144-M14 expression vector encoding the human DDX28 coding sequence (Genecopoeia). Selection was initiated 48 h post-transfection using 1 μg/mL puromycin or 400 μg/mL G418 ...
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bioRxiv - Immunology 2021Quote: ... CD4-conjugated antibodies were produced in stable cell lines via transient transfection of plasmid coding light chains using EndoFectin™ Max transfection agent (GeneCopoeia). Transfected cells were incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G (gifts from Didier Trono) were chemically transfected with the transfer plasmid into the 293Ta packaging cell line (Genecopoeia, LT008) using GeneTwin transfection reagent (Biomed ...
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bioRxiv - Cell Biology 2024Quote: ... and the transfer plasmid were chemically transfected using GeneTwin transfection reagent (Biomed, TG101) into the HEK293Ta packaging cell line (Genecopoeia, LT008). The cell culture medium was harvested after 2 days and filtered through a 0.45 μm filter ...
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bioRxiv - Bioengineering 2021Quote: ... U87ΔEGFR-luc was generated by lentiviral transduction of U87ΔEGFR cells using Lentifect™ lentiviral particles encoding for firefly luciferase and a puromycin-resistant gene (GeneCopoeia, LP461-025). Transduction was performed according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with an all-in-one CRISPR/Cas9 plasmid for CLN5 (plasmid number HCP202087-CG01-1-B, Genecopoeia, Rockville, MD). 72 hours post-transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Early passage (p1) of primary stroma cells were infected with Lentivirus particles for hTERT overexpression driven by EEF1A1 promoter (GeneCopoeia, LP730-025) according to the manufacture’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Luciferase counts were measured for cells infected with Spike-pseudotyped VSV-GFP/Fluc particles using Luc-Pair™ Firefly Luciferase HS Assay Kit (GeneCopoeia: LF009). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: CNTNAP4 KO and control-transfected cells were collected for the detection of the mutation using the T7 Endonuclease I Assay Kit (Genecopoeia, Rockville, MD). DNA was extracted from the samples using Quick-DNA™ Miniprep Kit (Zymo Research ...
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bioRxiv - Cell Biology 2021Quote: ... Lipofectamine 2000 has been used to transfect MIN6 cells with CRTC1 overexpressing plasmid (EX-Mm18750-Lv183) or with control plasmid (EX-EGFP-Lv151) (both from Genecopoeia, Rockville, MD, USA). Lipotoxic stress has been induced by using 0.5 mM of sodium palmitate (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... Plasmids were packaged into 3rd generation replication deficient lenti-virus in HEK-293T cells using the Lenti-Pac HIV Expression Packaging Kit (Genecopoeia, Rockville, Maryland, USA) following manufacturer’s protocol (Cat# LT001).