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Citations for Boston Biochem :
101 - 150 of 217 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... USP8 (wild-type or the mutants) was incubated with 25 ng/μl of Lys63-linked ubiquitin tetramers (Boston Biochem) in TBS with 10-mM dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2021Quote: USP8 immunoprecipitates or His-tagged FRIP8 precipitates were incubated with 1-μM ubiquitin-VME (Boston Biochem) in TBS supplemented with 1-mM DTT at 37°C for 5 min or 30 min ...
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bioRxiv - Immunology 2021Quote: ... E2 (1 μM, 1.2 μl, UbcH5a, Boston Biochem, E2-616), E3 (0.5 μM ...
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bioRxiv - Immunology 2021Quote: ... Ubiquitination assays were performed at 37 °C for 90 minutes using loaded E1 (~ 80 ng, 3 μl, Boston Biochem kit, K-995), E2 (1 μM ...
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) (Boston Biochem), Z-Leu-Leu-Glu-βNA (Z-LLE-βNA) ...
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bioRxiv - Biochemistry 2021Quote: polySUMO2 chains (#ULC-220, Boston Biochem) were directly labeled with Cy3 following the manufacturer’s guidelines (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... polySUMO2 (# ULC-210) and polySUMO3 (#ULC-220) – were purchased from Boston Biochem. Recombinant 6xHis-XLF (NBP2-23291) ...
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bioRxiv - Cell Biology 2021Quote: ... 200 nM UbcH13– Uev1a E2 complexes (Boston Biochem), 0.1ug recombinant human MUL1 Protein (H00079594-P01 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50 μM human ubiquitin (Boston Biochem), 4 mM ATP and 1 mM DTT in 20 mM MOPs ...
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bioRxiv - Synthetic Biology 2021Quote: Ubiquitination assays were performed as previously described 45 in the presence of 0.1 μM purified human UBE1 (Boston Biochem), 4 μM human UbcH5α/UBE2D1 (Boston Biochem) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
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bioRxiv - Plant Biology 2021Quote: ... Each 30 μL reaction included approximately 50 ng of yeast E1 (Boston Biochem), 250 ng of E2 (AtUBC10) ...
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bioRxiv - Microbiology 2021Quote: ... 0.25 mM E2 (cdc34; Boston Biochem), and 2.5 μg/ml ubiquitin (Boston Biochem ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg/ml ubiquitin (Boston Biochem) in the presence of purified BgaA-T and its variants ...
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bioRxiv - Microbiology 2021Quote: ... The SCFFBW7 E3 ligase complex was incubated with recombinant 0.1 mM E1 (UBE1; Boston Biochem), 0.25 mM E2 (cdc34 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Bioengineering 2021Quote: ... reaction mixture was supplemented with proteasome inhibitor MG132 (20 μM; Boston Biochem; #I-130). Reaction mixtures were incubated at 28°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng E1 (Boston Biochem, USA), 250 ng E2 (Boston Biochem ...
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bioRxiv - Cell Biology 2021Quote: ... energy regeneration solution (B-10, Boston Biochem) in assay buffer were carefully pipetted directly on the arrays ...
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bioRxiv - Cell Biology 2021Quote: ... K48-or K63-linked diubiquitins (Boston Biochem) in 20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... The treated cells were incubated with the 5 µM proteasome activity probe Me4BodipyFl-Ahx3Leu3VS (Boston Biochem) for 2 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... RPN2 (Boston Biochem AP-104, 1:1000), RPN13 (custom made13 ...
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bioRxiv - Biochemistry 2021Quote: ... human proteasomes were treated with ubiquitin-vinyl-sulfone (UbVS, Boston Biochem). UbVS was added to the resin at 1-1.5 mM ...
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bioRxiv - Biochemistry 2021Quote: ... Peptidase activities of purified immunoproteasomes were assayed using fluorogenic peptide substrates Ac-ANW-AMC for PSMB8 and Ac-PAL-AMC for PSMB9 (Boston Biochem). The measured activity was normalized against total protein concentration ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were synthesized by solid-phase synthesis through iterative cycles of coupling and deprotection using the previously optimized procedure.27Ub-AMC and ISG15-AMC were purchased from Boston Biochem (catalog # U-550-050 and UL-553-050 respectively).
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bioRxiv - Plant Biology 2021Quote: ... 1.25 µg Flag tagged ubiquitin (Boston Biochem), 2 µg purified GST-PUB25 and PUB26 or HIS-PUB26 were added to 30 µL of ubiquitination reaction buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 250 ng E2 (Boston Biochem, USA). Sterile water was added to make up the solution to 30 μL ...
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bioRxiv - Microbiology 2021Quote: The selectivity of the most potent inhibitors was tested against the human deubiquitinating enzymes USP7 and USP14 (Boston Biochem). Assay conditions were similar to the PLpro primary assay ...
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bioRxiv - Microbiology 2021Quote: ... with the following substitutions: USP7 assays contained 4 nM USP7 and 0.5 uM Ub-AMC (Boston Biochem); USP14 assays contained 1.7 uM USP14 ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Plant Biology 2021Quote: ... Ubiquitination reaction mixtures contained 50 ng yeast E1 (Boston Biochem), 50 ng rice 6xHis-Rad6 (E2) ...
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bioRxiv - Biochemistry 2021Quote: ... Ub-AMC (Boston Biochem) was used at a concentration (0.5 to 1 μM ...
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bioRxiv - Biochemistry 2021Quote: Ub-AMC (Boston Biochem, Cat. # U-550)
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bioRxiv - Biochemistry 2021Quote: Suc-LLVY-AMC (Boston Biochem, Cat. # S-280)
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...
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bioRxiv - Biochemistry 2021Quote: ... Boc-L-R-R-AMC (trypsin-like activity) and Z-L-L-E-AMC (caspase-like activity) (Boston Biochem). The proteasome samples were incubated with 50 μM AMC-peptide in the respective purification buffers (standard or the exogenous nucleotide depleted buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μM Ub-VS (Boston Biochem) was added to the proteasomes binding resin and incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2021Quote: Ubiquitin-AMC (Ub-AMC; Boston Biochem) hydrolysis assay was used to verify the wild-type and mutant USP14 deubiquitylating activities in the human proteasome ...
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bioRxiv - Biochemistry 2021Quote: ... 1.4 μM WW-HECT and 1 mg/ml ubiquitin (Boston Biochem) were incubated in reaction buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM E1 (Boston Biochem), 2 µM UbcH7 and 1.5 µM GST-RnParkin ...
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bioRxiv - Biochemistry 2021Quote: ... using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1, Boston Biochem Inc.), 0.5 mM ATP and 10 mM MgCl2 in 50 mM Tris/HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µM Ub (Boston Biochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... Tetra-phospho-ubiquitin chains (pUb)4 were obtained from Boston Biochem.
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bioRxiv - Biochemistry 2021Quote: ... WT or mutant with an excess of UbVS (Boston Biochem) in 25 mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... inhibition was evaluated with full-length STAMBP (Boston Biochem #E-549) (activated with STAM1 (Boston Biochem #E-550) ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence of hydrolyzed K63-linked diUb (TAMRA/QXL position 3 labeled, Boston Biochem #UF-330) was measured on a BioTek synergy LX plate reader equipped with a red filter cube assembly (ex ...
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bioRxiv - Biochemistry 2021Quote: ... inhibition was evaluated with full-length STAMBP (Boston Biochem #E-549) (activated with STAM1 (Boston Biochem #E-550)) and STAMBPL1 (Boston Biochem #E-551) ...