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Citations for Boston Biochem :
1 - 50 of 217 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The in vitro ubiquitination reaction was performed in a 30 μl mixture containing 200 ng E1 enzyme (BB-E-304-050, Boston Biochem), 200 ng E2 enzyme (BB-E2-616-100 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme) ...
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bioRxiv - Cell Biology 2024Quote: ... 200 ng E2 enzyme (BB-E2-616-100, Boston Biochem), 5 mg His-ubiquitin (BB-U-530 ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... and the rest was rotated overnight at 4°C with 50 µL commercial ubiquitin-conjugated TUBE beads (Boston Biochem). The beads were washed three times with 10 ml high salt TAP buffer (50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Neuroscience 2024Quote: ... non-phosphorylated PRKN (Boston Biochem, E3-160) and p-S65-Ub monomer (Boston Biochem ...
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bioRxiv - Neuroscience 2024Quote: ... Their nonphosphorylated counterparts (Boston Biochem, UC-210b and UC-310b) were used as negative controls ...
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bioRxiv - Neuroscience 2024Quote: Recombinant p-S65-PRKN (Boston Biochem, E3-166), non-phosphorylated PRKN (Boston Biochem ...
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bioRxiv - Neuroscience 2024Quote: ... and K63 (Boston Biochem, UC-350) p-S65-Ub tetramers were serially diluted to a final concentration of 10 fg/ml to 1 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and p-S65-Ub monomer (Boston Biochem, U102) were initially dissolved in RIPA buffer (stock concentration of 1 µg/µl) ...
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bioRxiv - Neuroscience 2024Quote: ... K48 (Boston Biochem, UC-250) and K63 (Boston Biochem ...
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bioRxiv - Cell Biology 2024Quote: ... The DUBs used in this study are USP2 (Enzo, BML-UW9850-0100, 1 µM and Boston Biochem, E-504-0650, 1 µM), vOTU (1 µM) ...
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bioRxiv - Immunology 2024Quote: ... K63 and linear ubiquitin chains were purchased from Boston Biochem.
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bioRxiv - Microbiology 2024Quote: ... MgCl (1 mM) and 1x Ubiquitination buffer (Boston Biochem; Table S4) were added and the reaction was incubated at 30°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with cold PBS and lysed in 300 µL 1x Ubiquitination buffer (Boston Biochem; Table S4) containing 1% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... Non-phosphorylated Ub protein (Boston Biochem, U-100H), free non-phosphorylated ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant of each single B cell well was screened for antigen specificity through direct ELISA for targeting full-length monomeric p-S65-Ub protein (Boston Biochem, U-102), free p-S65-Ub peptides 1 and 2 and BSA-conjugated p-S65-Ub peptides 1 and 2 (provided by 21st Century Biochemicals) ...
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bioRxiv - Biochemistry 2024Quote: Various combinations of E1 (100 ng; Boston Biochem), E2 (220 ng of CDC34 and 110 ng of UBCH5C) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteasome inhibitor MG-132 was from Boston Biochem. Cell Counting Kit-8 (CCK- 8 ...
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bioRxiv - Microbiology 2024Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Monoubiquitin and K63 linked Tetraubiquitin were purchased from Boston Biochem (Catalog # ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were then resuspended with 30 μl of 1uM K48-linked tetraubiquitin (Boston Biochem) diluted with 2xELB-sucrose-BSA-NP-40 + 5mM DTT and rotated on the wheel at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... with 200 μM FLAG-ubiquitin (Boston Biochem) and 0.2 μM UBE1 in reaction buffer (30 mM HEPES pH7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... pre-charged E2 enzyme was prepared by incubating 10 μM UBCH5C (Boston Biochem) with 200 μM FLAG-ubiquitin (Boston Biochem ...
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bioRxiv - Microbiology 2024Quote: ... washed and homogenized in Lysis Buffer (1x Ubiquitination buffer (Boston Biochem), 1x protease inhibitor (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... energy regeneration buffer (Boston Biochem), MgCl (1mM ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were run in duplicates and each plate had an organ specific pink1-/- sample included as a negative control as well as a recombinant K48 p-S65-Ub tetramer (Boston Biochem, UC-250) as a positive control ...
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bioRxiv - Biochemistry 2024Quote: A ubiquitin-AMC (Ub-AMC; Boston Biochem) hydrolysis assay was performed to verify the deubiquitylation activity of USP14 in the presence of different concentrations of NaCl or KCl ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... 25nM E1 enzyme (UBE1, Boston Biochem), and 125nM E2 enzyme (UbcH5c ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... 5 uM ubiquitin (Boston Biochem), 25nM E1 enzyme (UBE1 ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 mM MgATP solution (Boston Biochem B-20) in E3 ligase reaction buffer (Boston Biochem B-71) ...
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bioRxiv - Biochemistry 2024Quote: ... 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM TSF-D-type cyclins-CDK4EE complex or GST-cyclin D1 C-termini ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... and 125nM E2 enzyme (UbcH5c, UBE2DE3, Boston Biochem or expressed) and 62.5 nM variant PRC1 were combined in 1× UB buffer (30 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2024Quote: Bacterially purified RNF4 was purchased from Boston Biochem (#E3-210), Image-iT™ Lipid Peroxidation Kit from Thermo Fisher Scientific (#C10445) ...
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bioRxiv - Biochemistry 2024Quote: ... 1.5 μM UBCH5C (Boston Biochem E2- 627), 0.3 μM purified AMBRA1WD40-DDB1-Cullin4-RBX1 or AMBRA1FL-DDB1-Cullin4-RBX1 complex ...
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bioRxiv - Biochemistry 2024Quote: ... in E3 ligase reaction buffer (Boston Biochem B-71). The reaction was incubated at 37 °C for 2 h and analysed by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: The ubiquitination assays were performed in a 25 μL reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2- 627) ...
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bioRxiv - Biochemistry 2024Quote: ... 1.4□μM WW-HECT and 1□mg mL−1 ubiquitin (Boston Biochem) was incubated in reaction buffer (50□mM Tris-HCl [pH 7.5] ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.5 μg of E3 (His-XIAP) in a total reaction volume of 30 μL Nedd8 conjugation Rxn Buffer Kit (Boston Biochem, SK-20). Nedd8 (UL-812) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were then incubated for 1h at 37°C with 0.5mM HA-Ub-VS (Boston Biochem), which label active DUBs by covalently binding with their enzymatic site ...
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bioRxiv - Cell Biology 2024Quote: ... His- XIAP were purchased from Boston Biochem. Samples were incubated at 30 ℃ for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... UBE2D1 and UBE2A used for in vitro ubiquitination reactions were purchased from Boston Biochem. UBE2A was also purchased from UBPBio (C1101) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was inhibited via addition of 200 μM MG262 (Boston Biochem). Egg extracts were supplemented with recombinant proteins as detailed below and incubated for 10 min at RT before addition of plasmid DNA unless otherwise stated ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged ubiquitin mutants (Boston Biochem) were added at final concentrations of 1 µg/µL ...
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bioRxiv - Microbiology 2023Quote: ... The bead-bound PfSkp1GFP and PfCullin-2/cDDHA immunoprecipitates were assessed for ubiquitination activity using a ubiquitin conjugation initiation kit according to the manufacturer’s instructions (Boston Biochem). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
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bioRxiv - Biochemistry 2023Quote: ... 25 ×106 cells per condition) and subjected to immunoprecipitation using 5 µg of ISG15 antibody (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 0.6 μM ubiquitin (Boston Biochem, U-120), 225 nM E1-UBE-1 (Boston Biochem ...
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bioRxiv - Cell Biology 2023Quote: ... Reactions were started using E3 ligase MDM2/HDM2 400 ng (Boston Biochem, E3-204), incubated for 3 hours at 30°C in a water bath ...
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bioRxiv - Cell Biology 2023Quote: ... 5mM Mg-ATP solution (Boston Biochem, B-20), 0.5 mM DTT ...