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Citations for Sino Biological :
701 - 750 of 945 citations for Mouse Anti SARS Coronavirus Nucleoprotein Antibody 3863 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... with 5×105 TCID50 of recombinant MeV-derived vaccine virus in 200 μl volume or subcutaneously (s.c.) with 10 μg recombinant SARS-CoV-2 S protein (Sino Biological Europe, Eschborn, Germany) adjuvanted with 500 μg aluminum hydroxide (Allhydrogel adjuvant 2% ...
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bioRxiv - Microbiology 2021Quote: Biotinylated SARS-CoV-2 Spike D614G protein (Spikebiotin, in-house generated) or the biotinylated SARS-CoV-2 Spike receptor-binding domain (RBDbiotin, Sino Biological, 40592-V08B) were incubated with Alexa Fluor® 647 streptavidin (AF647-strep ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 PSD and pCMV3 containing the SARS-CoV-2/COVID-19 Spike gene were co-transfected into 7 x 105 293F cells using Sinofection (Sino Biological, Beijing, China). The medium was replaced with fresh DMEM containing 10% FBS after overnight incubation ...
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bioRxiv - Immunology 2021Quote: ... 1μg of formalin and heat inactivated SARS-CoV 2 adjuvanted in Alhydrogel (Brenntag) 1% or 1.5 μg of recombinant SARS-CoV-2 spike protein (S1+S2 ECD, His tag; Sino Biological, Cat. 40589-V08B1) adjuvanted in Alhydrogel (Brenntag ...
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bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used: rabbit anti-ACE2 (Sino Biological Inc, 10108-RP01, 1:100), rabbit anti-SARS-CoV-2 N protein (Rockland ...
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bioRxiv - Genomics 2020Quote: ... Cells were resuspended in FC buffer and anti-M13 antibody (Sino Biological, 11973-MM05T-50) was added at 1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... A primary anti-ACE2 rabbit polyclonal antibody (1:1000, 10108-T26, Sino Biological, Beijing, CHN) and a goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... bound Nb-Fc were detected with a HRP-conjugated anti-IgG1 Fc antibody (Sino Biological). After incubation for 2 hr at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... the blocking buffer was replaced with the primary anti-ACE2 antibody (10108-T24, Sino Biological) (1/1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
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bioRxiv - Microbiology 2020Quote: The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the pVSV-spike expression plasmid was constructed by PCR amplification of the full-length human codon-optimized S gene from pCMV3-SARS-CoV-2 S expression plasmid (Sino Biological, Cat# VG40588-UT) that was used to replace the VSV-G (Glycoprotein ...
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bioRxiv - Microbiology 2021Quote: ... Untagged and double-tagged SARS-CoV-2 spike variants were generated based on a template full-length pCMV3-SARS-CoV-2 Spike (codon-optimized, Sino Biological, cat # VG40589-UT) plasmid that has translated amino acid sequence identical to QHD43416.1 (GenBank) ...
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bioRxiv - Genetics 2020Quote: ... Loaded sensors were dipped into recombinant SARS-Cov-2 His-tagged Spike protein (D614 or D614G, Sino Biological, Cat # 40591-V08H and 40591-V08H3).
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Immunology 2021Quote: ... and blotted onto nitrocellulose membranes which were probed with the SARS-CoV2/2019-nCoV Spike rabbit polyclonal Ab (PAb, cat# 40591-T62, Sino Biologicals; dilution 1:1,000) followed by anti-Rabbit IgG-HRP conjugated antibody (1:15,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... VSV-SARS-1-GFP single cycle particles were produced in Expi293 cells through transfection per manufacturer’s instructions with the SARS-CoV-1 spike expression plasmid (Sino Biological, Cat #VG40150-G-N) containing a 19-aa deletion in the cytoplasmic tail ...
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bioRxiv - Microbiology 2020Quote: A full-length wild-type pCMV3-SARS-CoV-2 Spike (S1+S2)-long (termed as pCMV-S, codon-optimized, Sino Biological, cat # VG40589-UT) plasmid was used as a template to generate tagged pCMV-S ...
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bioRxiv - Immunology 2021Quote: ... Fractions which contain HexaPro protein were pooled and analyzed with SDS-PAGE and western blot against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or pooled convalescent sera ...
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bioRxiv - Cell Biology 2024Quote: ... The expression vector for the SARS-CoV-2 Spike protein (pCMV3-2019-nCoV-Spike (S1+S2)-long) was purchased from Sino Biological (VG40589-UT, China). The expression vector for ACE2 was purchased from Miaolinbio (pEnCMV-ACE2 (human)-3×FLAG ...
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bioRxiv - Microbiology 2022Quote: ... The plate was washed with buffer and 100 µl HRP-conjugated anti-his antibody (Sino Biological) was added to each well and incubated at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Subsequently membrane was hybridized with rabbit neutralizing anti-RBD antibody (Sino Biological, Cat. No. 40592-R001) or human derived RBD neutralising antibodies in blocking solution for 1h at room temperature with constant shaking ...
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bioRxiv - Microbiology 2020Quote: ... anti-M13 bacteriophage antibody conjugated with HRP (dilution factor 1 : 5000; Sino Biological, #11973-MM05T-H), was added into the above plates (150 μL/well ...
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bioRxiv - Microbiology 2021Quote: ... Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000 ...
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bioRxiv - Microbiology 2021Quote: ... an anti-M13 bacteriophage antibody with HRP (1:10000 dilution, cat.# 11973-MM05T-H, Sino Biological) was added and incubated at 37 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000 ...
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bioRxiv - Microbiology 2023Quote: ... antibodies, anti-HCoV-229E spike (PAB21477-100, LGC NAC Company) or nucleocapsid (40640-T62, Sino Biological) antibodies ...
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bioRxiv - Immunology 2023Quote: ... Either the horseradish peroxidase (HRP)-conjugated anti-M13 antibodies (Sino Biological, 11973-MM05T-H, 1:4,000) or HRP-conjugated rabbit anti-human IgG Fc antibodies (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... consisting of SARS-CoV-2 residues Arg319—Phe541 fused to a murine IgG1 Fc domain was purchased from Sino Biological (RBD-Fc, Cat#: 40592-V05H). For kinetic interaction analysis of RBD escape mutants and sybodies ...
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bioRxiv - Microbiology 2020Quote: ... China) were coated with 2 μg/mL (50μL/well) SARS-CoV-2 Spike Protein (S1 Subunit, His tag) (Sino Biological, China, cat no:40591-V08H) in carbonate bicarbonate buffer pH9.6 and the plates were incubated at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... was quantitated then added at stoichiometric ratios (starting at 1:1) to 170 ng (750 fmol) of SARS-CoV-2 S1 trimer complex (Sino Biological, Cat: 40591-V08H; RU171) that had been dye-labeled with Dy-549P1 NHS ester in a total volume of 100 μL ...
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bioRxiv - Genetics 2021Quote: ... Total EGF Receptor (10001-R021) and anti-EGFR-PE (352904) antibodies were purchased from Sino Biological (China) and BioLegend (USA) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose prior to Western blotting with anti-S1 polyclonal rabbit antibody (Sino biological) and Goat anti-rabbit IR-Dye800CW (Odyssey) ...
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bioRxiv - Bioengineering 2023Quote: ... plates were washed as before and 1:2,000 anti-M13-HRP antibody (Sino Biological #11973-MM05T-H) in 3MPBS was added to the wells ...
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bioRxiv - Immunology 2021Quote: ... 96 well NUNC MaxSorb flat-bottom plates were coated with 3.99 μg/ml recombinant SARS-CoV-2 Wuhan-Hu-1 spike RBD protein (Sino Biological, Beijing, PR China, Cat. 40592-V08B), 1 μg/ml SARS-CoV-2 Wuhan-Hu-1 nucleocapsid protein (Sino ...
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bioRxiv - Microbiology 2021Quote: ... 96-well plates were coated overnight with either 1 μg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag, Sino biological, Cat. No. 40592-V08B-100) or recombinant SARS-CoV-2 Nucleocapsid His Protein ...
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bioRxiv - Bioengineering 2023Quote: Nectagen’s nanoCLAMP phage display library NL-21 was panned as described (Suderman et al. 2017) against SARS CoV 2 Spike protein RBD (Sino Biologicals, S1RBD-Fc, cat#40592-VO2H) for rounds 1 and 2 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated mouse FcγRs (Sino Biological) were immobilized onto a Streptavidin sensor chip ...
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... and then membrane incubated with mouse anti-influenza A virus NP protein monoclonal Ab (Sino biological Company, China; 1: 10,000, 1 h) or mouse anti-influenza polyclonal anti-body (1 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1 hour at RT with 1:1,000 of anti-spike antibody (Rabbit MAb, Sino Biological, Beijing, CN) diluted in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was verified by Coomassie Blue staining and western blot analysis using anti-His antibody (Sino Biological).
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bioRxiv - Immunology 2019Quote: ... 3 nM mouse PD-L1-His/9 nM mouse CD80-His (Sino Biological, catalog 50446-M08H) combined ...
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bioRxiv - Microbiology 2020Quote: ... rACE2 (mouse-Fc tagged, Sino Biological) proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... rACE2 (mouse Fc tagged, Sino Biological) proteins were immobilized to a protein G sensorchip (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... mouse CD25-expressing plasmid (Sino Biological) was transiently transfected into HEK 293T/17 adherent cells using PEI 25K (ratio 3:1 ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated at 4°C overnight with rabbit anti-CoV-2 RBD polyclonal antibody (Cat# 40592-T62, Sino Biological) or anti-FLAG M2 peroxidase antibody (Cat# A8592 ...