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Citations for World Precision Instruments :
401 - 450 of 468 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Neuroscience 2023Quote: ... and 26-gauge needle (Hamilton) at a flow rate of 100 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
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bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid was delivered using a glass pipette at a flow rate of 50 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2023Quote: ... Electrical hindpaw stimulation was applied with 1 msec 5 mA current pulses using an isolated current stimulator (A382, World Precision Instruments, Sarasota, Florida). For each stimulation condition ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...
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bioRxiv - Neuroscience 2021Quote: ... +/−2.6mm ML, −4.5mm DV) using a 10μL syringe (nanofil, World Precision Instrument, USA) with NanoFil Needle (NF33BV-2, World Precision Instruments, USA) at a rate of 100nL/min (Micro4 pump ...
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bioRxiv - Neuroscience 2021Quote: ... then mounted lateral-side up on a thin layer of 1.5-2% low-melt agarose in a tissue culture dish with a cover-glass bottom (World Precision Instruments) and covered in E3 media ...
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bioRxiv - Neuroscience 2021Quote: ... A long tempered small tip (2 — 3 μm in diameter) patch pipette (∼3 MΩ; borosilicate glass, WPI, GBF 150-86-10) was used to pressure inject the dye into the extracellular space at 4 — 6 psi for 14 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... VP neurons were visualized using an Olympus BX51WI microscope and recorded from using glass pipettes (1.3-2 MΩ, World Precision Instruments) filled with internal solution (in mM ...
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bioRxiv - Cell Biology 2023Quote: ... was injected using a 5-µl Hamilton syringe at a rate of 0.25µl/min during 2 min with a mini-pump (UltraMicroPump III, World Precision Instruments, USA). The same volume of the dye Evans Blue (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... a 2-mm diameter craniotomy was then made over the fiducial mark and covered with KwikCast (World Precision Instruments, FL, USA). The mouse then recovered overnight before the recording session.
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bioRxiv - Cell Biology 2022Quote: ... TEER was measured with was monitored daily using an epithelial tissue volt ohmmeter (EVOMX) with STX-2 chopsticks (World Precision Instruments). TEER measurements were calculated in ohms cm2 after subtracting the blank value for the membrane insert ...
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bioRxiv - Neuroscience 2023Quote: ... 1474) and a 75-200 nL volume of viral vector was injected using a microinjector pump (World Precision Instruments, UMP3T-2) with an attached 10-μL NanoFil syringe (World Precision Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Neuroscience 2024Quote: ... The craniotomy was covered with agarose (2% in physiological Ringer solution) then sealed with silicon elastomer (Kwik-Cast, World Precision Instruments). This craniotomy was used for acute recording for 2-3 consecutive days (with the probe lowered to a new location each time) ...
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bioRxiv - Neuroscience 2024Quote: ... mounted on a microinjection syringe pump connected to a digital Micro2T controller (Model UMP3T-2; World Precision Instruments, Sarasota, FL, USA). After each injection ...
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bioRxiv - Neuroscience 2024Quote: ... we filled a glass pipettes (2-3 MΩ) with aCSF and delivered monophasic stimulus pulses (2-15 mV, 100 μs/pulse) using a stimulus isolator (A360, WPI, USA). For L2/3 stimulation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SM preparations were fixed between a stationary stainless steel hook and an isometric force transducer (AE 801, SensoNor A/S, Norten, Norway) coupled to an AD converter Lab-Trax 4/16 (World Precision Instruments, Inc., Sarasota, FL, USA). The data were continuously recorded using DataTrax2 (World Precision Instruments ...
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bioRxiv - Physiology 2021Quote: The mixed sample was then transferred to a 5 mL syringe and perfused using an Aladdin AL-1000 syringe pump (World precision instruments, United Kingdom) through the ibidi slide ...
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bioRxiv - Cell Biology 2023Quote: ... The micropipette was bent to a desired degree by heating it over an alcohol lamp and was filled with phosphate-buffered saline (PBS) using the MicroFil (World Precision Instruments, MF34G-5). The micropipette was installed to a micropipette holder ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with 3% isoflurane (Aerrane, Henry Schein) for 2 min and then transferred to the stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% and monitored the same way as described above for IHKA injection ...
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bioRxiv - Neuroscience 2021Quote: ... was inserted in the dorsal horn to carefully deliver CAV virus at 2 nl/s with the aid of electronically controlled nanoinjector (Nanoliter 2010, WPI, FL, US). Three injections ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 min in a rectangular transparent plexiglass box (induction chamber) and then transferred to a stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% ...
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bioRxiv - Microbiology 2021Quote: ... The dorsal side of their ears was then either exposed to mosquito bites for two minutes or injected with 0.2 μL of sporozoite suspension using a micro syringe (NanoFil 10 μL syringe mounted with a 35G beveled needle, World Precision Instruments), yielding fields of view containing in average 100 parasites ...
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bioRxiv - Neuroscience 2021Quote: ... 40 nl of one of the viral vectors listed above at a titer of 2*1012 particles/ml was injected per site (PMd, amv dlPAG) using a 10 μl nanofil syringe (World Precision Instruments) at 0.08 μl/min ...
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bioRxiv - Neuroscience 2021Quote: ... we used four individual samples (exceptions: SST RML 10 WPI: n = 3; PV NBH 10 WPI: n = 3; Astro RML 18 WPI: n = 2). RNA was converted to cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche Aplied Science) ...
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bioRxiv - Genetics 2020Quote: ... phenol red was injected into the yolk of 1- to 2-cell-stage embryos with a Pneumatic PicoPump pv280 (World Precision Instruments). After injection ...
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bioRxiv - Neuroscience 2021Quote: ... Approximately 1 µl (2 x 1010 GC/hemisphere) virus was dispensed using a NanoFil syringe with a 33G beveled needle (World Precision Instruments). The other hemisphere was injected in the same way ...
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bioRxiv - Neuroscience 2023Quote: ... 20 nl of the Cre-dependent helper virus was injected to the HDB (bilateral, n = 2; unilateral, n = 1) using a Nanoliter 2010 microinjection pump (World Precision Instruments). The injection coordinates were defined by a stereotaxic atlas (Paxinos and Franklin ...
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bioRxiv - Cell Biology 2024Quote: ... and injected with 3 μl of AAV9-hSyn-iCre (titer 1 x 1013 to 2 x 1014) into the right lateral ventricle using a nanoliter injector (World Precision Instruments; Micro4 MicroSyringe Pump Controller connected with Nanoliter 2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The virus solution (0.4-0.6 µL) was injected at a rate of 2 nL/s with a microinjection pump (either Neurostar NanoW or WPI NanoLiter 2010). The pipette was slowly withdrawn at least 10 minutes after the completion of the injection ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings from GPe neurons were obtained using micropipettes (2–4MΩ) made from 1.5 mm OD/1.12 mm ID borosilicate glass with a filament (World Precision Instruments, Sarasota, FL) pulled with a P-97 puller (Sutter Instruments Novato ...
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bioRxiv - Neuroscience 2023Quote: ... Three circular glass coverslip (2 of 3 mm and one 4mm coverslips) was affixed with a clear silicone elastomer (Kwik-sil, World Precision Instruments). A custom 3D-printed stainless steel headpost was attached on the skull and the rest of exposed skull area and the edge of the cranial window were covered using a dental acrylic (C&B Metabond) ...
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bioRxiv - Cell Biology 2024Quote: ... Briefly 0.1 to 0.2 x 106 cells were seeded in 2 ml of medium in 6-well dishes or 3cm glass-bottomed microscope dishes (World Precision Instruments) or 0.04 x 106 cells were seeded in 300µl of medium in chambers of an 8-well microscopy slide (Ibidi ...
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bioRxiv - Cell Biology 2024Quote: ... Briefly 0.1 to 0.2 x 106 cells were seeded in 2 ml of medium in 6-well dishes or 3cm glass- bottomed microscope dishes (World Precision Instruments) or 0.04 x 106 cells were seeded in 300µl of medium in chambers of an 8-well microscopy slide (Ibidi ...
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bioRxiv - Neuroscience 2024Quote: ... was connected by polyethylene tubing to a 2 µl Hamilton syringe mounted in a microinjection pump (Legato 130 Single Syringe I/W Nanoliter, World Precision Instruments). The syringe ...
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bioRxiv - Neuroscience 2024Quote: ... Viruses were pressure-injected using a 10-μl Hamilton syringe (model 1701 RN, Hamilton, Reno, UV) mounted on a motorized microinjector (UMP3T-2, WPI, Sarasota, FL) held by a manipulator (model 1460 ...
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bioRxiv - Neuroscience 2024Quote: ... which was achieved by passing 1 ms current pulses between the platinum-iridium electrodes of the stimulating chamber (Warner Instruments, PH-2) that were induced using a stimulus isolator (WPI, MODEL A382) whose output was controlled by the same custom Arduino-based circuit coupling imaging and stimulation ...
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bioRxiv - Cell Biology 2024Quote: ... Chips were then blow-dried and functionalized with air plasma for 2 min (Harrick Plasma, #PDC-001) to be bonded to functionalized glass-bottom dishes (WPI, #FD35-100). The resulting devices were baked using an oven for 1 hour at 60°C ...
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bioRxiv - Microbiology 2021Quote: ... cRNA was micro-injected into stage 5 or 6 oocytes using a Micro4TM micro-syringe pump controller and A203XVY nanoliter injector (World Precision Instruments, Sarasota, FLA, U.S.A.). Oocytes were maintained in oocyte Ringer’s buffer with added gentamycin (50 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized and electrolytic lesions were made through individual tetrode leads (5-30μA for 5s; stimulus isolator, World Precision Instruments or A-M Systems). Next ...
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bioRxiv - Neuroscience 2020Quote: ... with a 33-G beveled needle that was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, FL, USA). Other surgical procedures and viral injection was as described previously (15).
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bioRxiv - Neuroscience 2020Quote: ... at a rate of 0.2 μL/min with a glass micropipette connected to a Hamilton syringe controlled by stereotaxic syringe holder (SYS-Micro4, WPI, Sarasota, FL, USA). The needle was removed 10 min later ...