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Citations for World Precision Instruments :
351 - 400 of 468 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... oocytes were transferred to a 2 μl media droplet in a 35 mm glass bottom FluoroDish from WPI covered with 400-500 μl of oil ...
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bioRxiv - Neuroscience 2021Quote: Recording electrodes (resistance 2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, Fl) using a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2021Quote: ... Recording pipettes (2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, FL, USA) with a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were removed and injected one at a time with 2 μL (2μg/μl) DNA containing pAAV-CAG-mRuby2 and CMV-dnKv4.2 using pulled glass micropipettes (WPI) into the lateral ventricle ...
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bioRxiv - Neuroscience 2023Quote: ... AAV virus was then infused via a blunt 36-gauge stainless steel needle (World Precision Instruments NF36BL-2) connected to a microliter injection system (10 µL ...
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bioRxiv - Neuroscience 2023Quote: ... were pressure-injected into caudate nucleus (Interaural 9.75 mm) and putamen (Interaural 9.75 mm) by Hamilton syringe mounted into motorized microinjector (UMP3T-2, WPI) held by manipulator (Model 1460 ...
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bioRxiv - Neuroscience 2024Quote: ... the craniotomies were covered with agarose (2%) and subsequently sealed with silicone elastomer (Kwik-cast, World Precision Instruments). Recordings were performed daily for up to 3 days following the craniotomies.
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bioRxiv - Biophysics 2020Quote: ... The signal from the force transducer was amplified using Transbridge TBM4M and digitized using Lab-Trax-4 data acquisition hardware (WPI, Sarasota, FL, USA). Three phases could be distinguished on force curves recorded using LabScribe v3 (iWorks ...
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bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Test compounds were dissolved in aCSF and locally puff-applied with a patch pipette (4–6 MΩ) at low pressure (∼1 psi) controlled by a Pneumatic Picopump (PV821, World Precision Instruments, USA) for 20 to 90s ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
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bioRxiv - Neuroscience 2024Quote: ... Local field-potential (LFP) activity was recorded using a patch pipette prepared from thin-borosilicate glass (1.5mmOD, 0.84mmID, World Precision Instruments, cat.no. 1B150F-4) pooled and fire-polished on a Flaming/Brown micropipette puller (Sutter Instrument ...
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bioRxiv - Molecular Biology 2024Quote: Glass capillary tubes with an inner diameter about 0.2 mm were prepared from thin wall glass capillaries (TW100F-4, World Precision Instruments, Sarasota, FL), using puller (PC-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Constant current stimuli (0.5 μA-5 mA) were applied using a high-voltage stimulus isolator (A365R, World Precision Instruments, Sarasota, FL). The evoked mono and polysynaptic IPSCs were recorded simultaneously in GCs and SGC pairs ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... was injected iontophoretically (0.8—1.0 μA positive current pulses with a 7 s-on/off cycle for 5 min using A365, World Precision Instruments). The coordinates of the injection were [AP −2.9 ...
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Drugs were infused over 5 minutes intravenously via a tail vein catheter with an infusion pump (World Precision Instruments, Sarasota, FL). Focused ultrasound was applied after 2.5 minutes of drug infusion for 2.5 minutes (250 kHz ...
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bioRxiv - Neuroscience 2024Quote: ... We used 3−5 MΩ glass patch pipettes (capillary tubing with 1.5/0.75 mm OD/ID-World Precision Instruments, Sarasota, FL) that were pulled on a P-97 Flaming/Brown type micropipette puller (Sutter Instrument Co. ...
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bioRxiv - Cell Biology 2020Quote: ... on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100, WPI). 8 hr following transfection ...
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bioRxiv - Immunology 2021Quote: ... Barrier integrity of ALI cultures was monitored by measuring trans-epithelial electrical resistance (TEER, EVOM 2, World Precision Instruments). ALI cultures were validated for use in experiments based on microscopic appearance of appropriate ciliated morphology and TEER > 500 Ω*cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... TEER values were determined daily beginning on day-2 using an epithelial volt/ohm meter (EVOM2, World Precision Instruments) and STX2 series chopstick electrodes as described previously (23).
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bioRxiv - Neuroscience 2024Quote: ... For all surgical procedures mice were anesthetized using 1.5-2% isoflurane and were injected using a Kopf stereotaxic and World Precision Instruments (WPI) microinjection syringe pump with a 10 mL syringe and 33g blunt needle at 50-100 nL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were loaded into a 2% agarose gel mold and a pre-pulled glass needle (World Precision Instruments, Sarasota) was filled with 10 μL of the injection medium ...
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bioRxiv - Neuroscience 2023Quote: ... Item# WE30030.5A5) with 2-3 µm diameter and 0.5 MΩ impedance connected to DAM50 Extracellular Amplifier (World Precision Instruments); data were acquired with Clampex 10.7 (Molecular Devices) ...
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bioRxiv - Neuroscience 2024Quote: ... 600nl of vector per hemisphere was injected at 200nl/min into the lateral hypothalamic area (AP/DV/ML = ±1.6/-5.3/ ±0.9 mm) using a microinjection syringe pump (UMP3T-2 World Precision Instruments). For morphological analysis of astrocytes in the LHa viral titres were dropped ten-fold to 1011 GC/ml ...
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2024Quote: Molds for the device assembly were fabricated using PDMS and biopsy punches of 4 mm and 7 mm diameters (World Precision Instruments, Sarasota, FL). A large well was made in the PDMS with the 7 mm punch ...
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bioRxiv - Neuroscience 2024Quote: ... A Hamilton syringe (Hamilton 7635-01) was filled with either PBS or C9-ASO at 50 ng/μL and attached to a microinjection pump (World Precision Instruments Micro 4) on a stereotactic arm ...
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bioRxiv - Neuroscience 2024Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
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bioRxiv - Molecular Biology 2024Quote: ... The electrical resistance of cell monolayers was measured on day 4 (before stimulation) and day 7 (after 3 days of stimulation) using an EVOM2 epithelial Volt/ Ohm meter (World Precision Instruments, FL, USA). The resistance of cell monolayers was corrected by subtracting the resistance of wells without cells and normalized by multiplying by the effective well surface area to provide transepithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2021Quote: ... was injected into PR at 0.5 µl/min using G33 needle (Hamilton) connected to a 10 µl micro-syringe controlled by a micro-pump (WPI, UltraMicroPump 3). AM cells were recorded both before and 30 min after injection.
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2020Quote: Electrical stimulation was applied through ACSF filled glass pipette (tip diameter 3–5 µm) connected to a linear stimulus isolator (A395, World Precision Instruments) was used to deliver monopolar electrical stimulation (10–90 µA ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments). An agar bridge (1M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Neuroscience 2024Quote: All recordings were performed at 31–33°C with electrodes (3–5 MΏ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with one of two intracellular solutions (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0-2.3 mm lateral to midline and 2.0-2.5 mm posterior to bregma) at a rate of 60 nL/min at a concentration of 2 mM using a Nanoliter 2020 system (WPI). Optogenetic silencing of V1 was achieved by the use of mice in which channelrhodopsin-2 expression was driven by cre in parvalbumin positive interneurons (Ai32 x PVcre) ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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bioRxiv - Neuroscience 2024Quote: ... we applied 1-s pressure pulses (0.4-0.6 psi) every 2 s for 1 min by a microinjector (PV850, World Precision Instruments). In some experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon OrganoidsbioRxiv - Molecular Biology 2022Quote: ... TEER values were determined daily with an Epithelial Volt ohm meter 2 (EVOM2) epithelial volt/ohm meter (World Precision Instruments) and STX2 series chopstick electrodes.
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...