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Citations for World Precision Instruments :
801 - 840 of 840 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... neurons are sparsely plated in 3-4 spots on 35mm plates (Fluorodish, World Precision Instruments) coated with PDL/Laminin ...
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bioRxiv - Neuroscience 2024Quote: ... The mice were placed onto a closed-loop heat plate (ATC 1000, WPI, Berlin, Germany), to ensure a stable body temperature of 37° Celsius ...
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bioRxiv - Neuroscience 2020Quote: ... and a lateral ventricle of each embryo was injected using pulled glass capillaries and a microinjector (PV 820 Pneumatic PicoPump; World Precision Instruments, Sarasota, FL) with Fast Green (2 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Whole cell voltage and current-clamp recordings were performed at 32–34 °C with electrodes (3–5MΩ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with intracellular solution of the following composition (in mM) ...
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bioRxiv - Neuroscience 2020Quote: ... or ACSF (500 nl) were delivered unilaterally through a pulled glass pipette at a rate of 100 nl/min using an UltraMicroPump (World Precision Instruments, Sarasota, FL). Injection coordinates for mPFC ...
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bioRxiv - Neuroscience 2020Quote: ... AAVs or retrobeads were delivered unilaterally through a pulled glass pipette at a rate of 100 nl/min using an UltraMicroPump (World Precision Instruments, Sarasota, FL). Injection coordinates for VTA ...
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bioRxiv - Physiology 2020Quote: ... TEP was measured by impaling the tubule lumen with a sharp microelectrode pulled from double-barreled theta-glass (World Precision Instruments, Inc. FL, USA) with reference to the basolateral bath ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... One antenna of the restrained bee was then cut off from the pedicel and subsequently transferred to a custom-made antennal holder composed of two capillary glass (tip and reference) electrodes (World Precision Instruments, Inc., USA) filled with insect saline solution ...
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bioRxiv - Neuroscience 2022Quote: ... Gentle pressure was applied to the cover glass while the space between the coverslip and the remaining skull was filled with Kwik-sil adhesive (World Precision Instruments, FL, USA). A layer of vet-bond tissue adhesive (3M ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (3-6 MΩ) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
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bioRxiv - Cell Biology 2024Quote: ... pH of the media during the assays did not change and was confirmed using glass tipped pH microelectrodes (World Precision Instruments, Sarasota Fl, USA). Indo-1 stained cells were added into the microtiter plate at unform cell density across the plate and fluorescence (340/400:475 nm ...
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bioRxiv - Neuroscience 2024Quote: ... and a lateral ventricle of each embryo was injected using pulled glass capillaries and a microinjector (PV 820 Pneumatic PicoPump; World Precision Instruments, Sarasota, FL) with Fast Green (2 mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... HeLa Kyoto cells transiently expressing NLS-TagBFP for 24h were plated onto fibronectin-coated imaging dishes with a small 10 mm glass coverslip (World Precision Instruments, FD35-10) and allowed to adhere for one hour in 50 μl of DMEM media enriched with 10 % FBS and 1 % P/S ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 μL of viral solution was injected into the right dorsal striatum through a small hole (+0.6 mm anteroposterior and ±2.2 mm mediolateral from bregma and −2.2 mm dorsoventral from the brain surface according to the atlas of Paxinos and Franklin) using a glass micropipette connected to a Nanoliter 2020 injector (World Precision Instruments, FL, USA). The mice were perfused 3 weeks after AAV injection.
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bioRxiv - Neuroscience 2024Quote: ... 200 nL of rAAV-hSyn-GCaMP6f-WPRE-hGh polyA/ AAV-hSyn-GCaMP6s-WPRE-hGh polyA was injected at a rate of 100 nL/min using a glass capillary (NANOLITER2020 Injector, World Precision Instruments, FL, USA), 3.1 mm lateral to the midline ...
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bioRxiv - Neuroscience 2021Quote: ... Body temperature was maintained with a heating plate (ATC 1000; World Precision Instruments, Sarasota, FL, USA) for the duration of the session ...
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bioRxiv - Neuroscience 2022Quote: ... 3.7—4.2 mm from the pial surface (D 3.7—4.2)] using a glass pipette (tip diameter, 15—20 µm) through which air pressure pulses were delivered with a pressure injector (PV820, World Precision Instruments, Sarasota, FL, USA). The site of injection extended to the substantia nigra pars reticulata (SNpr) ...
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bioRxiv - Neuroscience 2020Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting 1D channels of 5 μm height and 5 μm width were sealed in 35-mm glass bottomed cell culture dishes (World Precision Instruments, Sarasota, FL, USA) using plasma surface activation ...
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bioRxiv - Neuroscience 2022Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2021Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.5 µL of 1% Fast Green was loaded into a glass micropipette (described above) and delivered with a picospritzer fluid injector (model PV830 Pneumatic PicoPump, SYS-PV830, World Precision Instruments, Sarasota, FL, USA). To target the presumptive NCM that migrates into the mandibular primordia (Fish et al. ...
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bioRxiv - Developmental Biology 2021Quote: Vivo-Morpholino solution was injected into the right side of quail mandibular primordia at HH18 with pulled glass micropipettes loaded into a PicoNozzle (5430-10) and a picospritzer fluid injector (PV830, World Precision Instruments, Sarasota, FL, USA). After injection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Whole cell current- and voltage-clamp recordings were performed with pipettes (3-7MΩ when filled with intracellular solution) made from borosilicate glass capillaries (World Precision Instruments, Hitchin, Herts, UK) pulled on a P-97 Flaming/Brown micropipette puller (Sutter ...
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bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
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bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 200 nL of AAV was injected into the ARC at a speed of 50 nL/minute using a glass capillary regulated by a micro syringe pump injector (UMP3; World Precision Instruments, Sarasota, FL, USA) in all experiments ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid was delivered using a glass pipette at a flow rate of 50 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2024Quote: ... which expresses eGFP under the control of a CAG promotor was injected (∼100 nl/min) into the cervical DRGs using a glass micropipette and nano-injector (World Precision Instruments, Inc., Sarasota, FL.). The glass micropipette was left in place 3-5 minutes after each injection to ensure adequate diffusion of adenovirus into the DRG ...
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bioRxiv - Neuroscience 2024Quote: ... was located over the primary motor cortex (based on stereotactic coordinates at 0.2 mm anterior to bregma and 1.2 mm lateral to midline) and marked with a pen to be carefully removed and replaced by a precut #1 square cover glass (World Precision Instruments, coverslip No. 1). Cyanoacrylate-based glue and dental cement were used to seal the edges of the cover glass ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...
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bioRxiv - Plant Biology 2022Quote: ... in 50 nl water or with 50 nl water alone as a control using a glass capillary attached to a semi-automated micro-injector (World Precision Instruments, Inc. Sarasota, FL, USA) according to Aguero et al. ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells plated onto 0.1% gelatin-coated FluoroDish™ culture plates (35 mm, World Precision Instruments Inc.) were rinsed twice with DMEM (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... ruIs57[unc-119(+) pie-1::GFP::tubulin]) young adult hermaphrodites using needles pulled from borosilicate glass capillaries (World Precision Instruments,1.0 mm OD, 0.58 mm ID, with filament) with a P-87 micropipette puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... Body temperature was maintained at 37°C using a heating plate connected to a temperature controller (World Precision Instruments, USA). A rectal probe was used to monitor the animal’s body temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Bowl-shaped aluminum foil was attached to the head plate on the animal with silicone elastomer (Kwik Cast, World Precision Instruments; Dentsilicone-V, Shofu, Japan) and the space above the animal’s head was covered with light-shielding cloths ...