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Citations for World Precision Instruments :
651 - 700 of 702 citations for BODIPY FL DHPE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and a lateral ventricle of each embryo was injected using pulled glass capillaries and a microinjector (PV 820 Pneumatic PicoPump; World Precision Instruments, Sarasota, FL) with Fast Green (2 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV injection was done over 5 min using 10-μL Hamilton syringes (Hamilton, Reno, NV) driven by a microsyringe pump attached to an automated controller (World Precision Instruments, Sarasota, FL). On the same day as the intracranial injection ...
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bioRxiv - Neuroscience 2024Quote: ... and transcardial perfusion with ice-cold 0.9% NaCl for 10mins at a constant flow rate of 6mL/min using a peristaltic pump (World Precision Instruments, Sarasota, FL, USA). Upon removal ...
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bioRxiv - Neuroscience 2024Quote: ... and transcardial perfusion with ice-cold 0.9% NaCl for 10 mins at a constant flow rate of 6mL/min using a peristaltic pump (World Precision Instruments, Sarasota, FL, USA). Brains were then sagittally bisected ...
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bioRxiv - Neuroscience 2024Quote: ... was delivered through a pulled glass pipette (Tip diameter 7 - 10 µm) for a total volume of 200 nL at a rate of 100-150 nL/min using an UltraMicroPump (World Precision Instruments, Sarasota, FL). Injection coordinates for the motor cortex ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 μL of viral solution was injected into the right dorsal striatum through a small hole (+0.6 mm anteroposterior and ±2.2 mm mediolateral from bregma and −2.2 mm dorsoventral from the brain surface according to the atlas of Paxinos and Franklin) using a glass micropipette connected to a Nanoliter 2020 injector (World Precision Instruments, FL, USA). The mice were perfused 3 weeks after AAV injection.
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bioRxiv - Neuroscience 2024Quote: ... 200 nL of rAAV-hSyn-GCaMP6f-WPRE-hGh polyA/ AAV-hSyn-GCaMP6s-WPRE-hGh polyA was injected at a rate of 100 nL/min using a glass capillary (NANOLITER2020 Injector, World Precision Instruments, FL, USA), 3.1 mm lateral to the midline ...
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bioRxiv - Neuroscience 2024Quote: ... Impedance was measured using an oscilloscope (TDS 2012C Tektronix, Beaverton, OR) and custom programmed software that triggered a stimulus isolator (A385 World Precision Instruments, Sarasota, FL) to produce a known 100 μA current square wave ...
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bioRxiv - Bioengineering 2024Quote: Bladder pressure was monitored with pressure transducers (DPT-100, Utah Medical Products, Midvale, UT) connected to each implanted catheter and an amplifier (TBM4M, World Precision Instruments, Sarasota, FL). The bladder pressure signal was recorded at 1 kHz ...
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bioRxiv - Cancer Biology 2024Quote: BUVEC-E6E7 monolayers were grown on the top of a transwell insert with 0.4 μm pores and transendothelial electrical resistance (TEER) was measured using an EVOM2 Volt/Ohm resistance meter (World Precision Instruments, Sarasota, FL, USA) as previously reported [4] ...
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bioRxiv - Neuroscience 2024Quote: PO2 measurements in the spinal cord were conducted using a fiber-optic microsensor with a 50 μm tip diameter (Optode, OxyMicro System, World Precision Instruments, FL, USA). The microsensor was implanted at 100µm deep into the cord in the anterior funiculus between L1 and L2 segments ...
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bioRxiv - Neuroscience 2022Quote: ... 3.7—4.2 mm from the pial surface (D 3.7—4.2)] using a glass pipette (tip diameter, 15—20 µm) through which air pressure pulses were delivered with a pressure injector (PV820, World Precision Instruments, Sarasota, FL, USA). The site of injection extended to the substantia nigra pars reticulata (SNpr) ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... Cells were grown until the formation of electrically tight epithelial monolayer was confirmed by measuring transepithelial resistance with EVOM2 Epithelial Voltohmmeter (World Precision Instruments, Sarasota, FL, USA). Short circuit current (Isc ...
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bioRxiv - Neuroscience 2021Quote: ... Silicone adhesive was applied around the working electrode to prevent cortical tissue damage from the heat of curing dental cement (World Precision Instruments, Sarasota, FL, USA). Skull screws ...
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bioRxiv - Neuroscience 2020Quote: ... Adult C57/BL6 mice anesthetized with isoflurane in O2 (Abbvie, Ludwigshafen, Deutschland) were placed in a stereotactic frame (Kopf, CA, USA) and warmed with a heating pad (World Precision Instruments, Sarasota, FL, USA). For virus injections ...
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bioRxiv - Neuroscience 2021Quote: ... We monitored ICP via the cisterna magna cannula connected to a transducer attached to a pressure monitor (BP-1, World Precision Instruments Inc., Sarasota, FL). In another set of experiments ...
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bioRxiv - Neuroscience 2020Quote: ... then a small volume of agonist-containing solution was puffed on the cells via a pneumatic pico-pump (PV820; World Precision Instruments, Inc., Sarasota, FL) with a brief pressure (10 psi ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Solutions were injected laterally between the penultimate and the last abdominal segment using a micromanipulator and a microsyringe pump injector (World Precision Instruments, Sarasota, FL, USA) under a dissecting microscope (n = 8 individuals per dose).
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bioRxiv - Neuroscience 2020Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting 1D channels of 5 μm height and 5 μm width were sealed in 35-mm glass bottomed cell culture dishes (World Precision Instruments, Sarasota, FL, USA) using plasma surface activation ...
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bioRxiv - Neuroscience 2022Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Bioengineering 2022Quote: ... The PDMS block was then removed from the mold and access ports were cored with a 1-mm biopsy punch (World Precision Instruments, Sarasota, FL, USA).
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bioRxiv - Microbiology 2022Quote: Consumption of O2 was measured using an ISO-OXY-2 O2 sensor attached to an APOLLO 4000 free radical analyzer (World Precision Instruments, Inc., Sarasota, FL) as described52 ...
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bioRxiv - Neuroscience 2021Quote: ... at a rate of 100 nl/min into the respective subareas using a 10 μl gas-tight Hamilton syringe (World Precision Instruments, Sarasota, FL, USA). To minimize the reflux of the injected volume ...
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bioRxiv - Neuroscience 2021Quote: ... Kainate was injected at a rate of 70 nl/ min with a 10 µl Nanofil syringe (34 GA blunt needle, World Precision Instruments, Sarasota, FL, USA). The syringe was kept in place for 2 min after the injection was completed to avoid liquid reflux.
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bioRxiv - Neuroscience 2021Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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Sense of absence: Spatial perception through active sensing by insect antennal mechanosensory systembioRxiv - Animal Behavior and Cognition 2021Quote: An airflow stimulus was provided to the cricket that was stationary for more than one second by a short puff of nitrogen (N2) gas from a plastic nozzle (15 mm diameter) connected to a PV820 pneumatic picopump (World Precision Instruments, Sarasota, FL, USA). By adjusting the delivery pressure of the picopump ...
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bioRxiv - Neuroscience 2020Quote: ... cone tipped 5μl stainless steel injector (SGE Analytical Science, Australia) over 3 min using an infusion pump (UMP3 with SYS4 Micro-controller, World Precision Instruments, Inc., FL, USA). The needle was left in place for 7 min to allow for diffusion of and reduce spread up the infusion tract ...
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bioRxiv - Physiology 2021Quote: ... the MEA was placed dorsally based on the location of brain and heart and fixed by a manual micromanipulator (M3301, World precision instruments Inc., Sarasota, FL) to reduce vibrations and noises ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.5 µL of 1% Fast Green was loaded into a glass micropipette (described above) and delivered with a picospritzer fluid injector (model PV830 Pneumatic PicoPump, SYS-PV830, World Precision Instruments, Sarasota, FL, USA). To target the presumptive NCM that migrates into the mandibular primordia (Fish et al. ...
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bioRxiv - Developmental Biology 2021Quote: Vivo-Morpholino solution was injected into the right side of quail mandibular primordia at HH18 with pulled glass micropipettes loaded into a PicoNozzle (5430-10) and a picospritzer fluid injector (PV830, World Precision Instruments, Sarasota, FL, USA). After injection ...
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bioRxiv - Neuroscience 2021Quote: ... was performed with a scalpel just above the right auditory cortex and covered with silicone oil and silicone casting compound (Kwik cast, World Precision Instruments, Inc. FL, USA) during the 2 h recovery period from the anesthesia.
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bioRxiv - Neuroscience 2022Quote: Whole cell patch clamp was performed on interneurons from the lumbar segments using 2–4 MΩ glass electrodes pulled from glass capillary tubes (Item #TW150F-4, World Precision Instruments, Sarasota, FL, USA) with a Flaming-Brown P-97 (Sutter Instrument Company ...
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bioRxiv - Neuroscience 2022Quote: ... Either scAAV2-eGFP or scAAV2-Cre was microinjected using a micropipette pulled to a diameter of 0.05 mm and a nanoinjector (World Precision Instruments, Inc., Sarasota, FL, USA). For each injection ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 200 nL of AAV was injected into the ARC at a speed of 50 nL/minute using a glass capillary regulated by a micro syringe pump injector (UMP3; World Precision Instruments, Sarasota, FL, USA) in all experiments ...
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bioRxiv - Biophysics 2024Quote: ... were individually microinjected with connexin cRNAs (20-40 ng) plus a Cx38 antisense oligonucleotide (20 ng) using the Nanoliter 2020 Injector system (World Precision Instruments, Sarasota, FL, USA). Following microinjection ...
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bioRxiv - Microbiology 2023Quote: ... were individually microinjected with GRA cRNAs (20-40 ng) or H2O (as a control) using the Nanoliter 2020 Injector system (World Precision Instruments, Sarasota, FL, USA). Following microinjection ...
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bioRxiv - Neuroscience 2022Quote: ... The open end of the GRIN lens at the integrated docking platform was sealed using a Kwik- Sil silicone compound (World Precision Instruments, Sarasota, FL, USA). After stitching ...
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bioRxiv - Neuroscience 2023Quote: ... were injected in the ventral horn with a 33-gauge needle on a microsyringe controlled by an UltraMicroPump and Micro4 Microsyringe Pump Controller (World Precision Instruments, Sarasota, FL, USA). A total of six injections were performed bilaterally from C4 to C6 ...
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bioRxiv - Neuroscience 2023Quote: ... The virus (AAV1/2-OXTp-hM4Dgi-mCherry or AAV1/2-OXTp-Venus or AAV1/2-OXTp-mCherry) was loaded into a 20μl NanoFil syringe fitted with a 33gauge needle (cat no. NF33BL, World Precision Instruments Inc, Sarasota, FL, USA). A final volume of 270nl was injected into the PVH (A-P −1.7mm ...
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Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... The DBS lead was fixed in by filling the craniotomy with adhesive silicone (KWIK-SIL, low toxicity silicone adhesive, World Precision Instruments, Sarasota, FL, USA). For safety ...
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bioRxiv - Neuroscience 2024Quote: ... which expresses eGFP under the control of a CAG promotor was injected (∼100 nl/min) into the cervical DRGs using a glass micropipette and nano-injector (World Precision Instruments, Inc., Sarasota, FL.). The glass micropipette was left in place 3-5 minutes after each injection to ensure adequate diffusion of adenovirus into the DRG ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Evolutionary Biology 2024Quote: The pERGs were amplified with a gain of x1000 and bandpass filtered between 1 Hz and 1 kHz with a differential amplifier (DAM50, World Precision Instruments Inc., FL, USA). Voltages were digitised at 20000Hz using a National Instruments data acquisition device (USB-6353 ...
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Action selection based on multiple-stimulus aspects in the wind-elicited escape behavior of cricketsbioRxiv - Animal Behavior and Cognition 2021Quote: The air-current stimulus used throughout the experiments was a short puff of nitrogen gas from a plastic nozzle (ø = 15 mm) connected to a pneumatic picopump (PV820, World Precision Instruments, Sarasota, FL, USA). One air-current nozzle was installed on the inside wall of the arena to be positioned on the same horizontal plane as the animal ...
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bioRxiv - Biophysics 2021Quote: ... to block or enhance a specific pathway the ear surface was gently cleaned and dried and a 4 µl solution containing a selected drug (Table 2) diluted in a PBS was microinjected in the earlobe skin using a 10 µl NANOFIL syringe (World Precision Instruments Inc., Sarasota, FL, USA) equipped with a gauge 33 needle ...
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bioRxiv - Neuroscience 2021Quote: ... Extracellular recordings were obtained using tungsten electrodes (TM31C40KT, 4-MΩ impedance at 1 kHz or TM31A50KT, 5-MΩ impedance at 1 kHz; World Precision Instruments Inc., Sarasota FL, USA). All recordings were pre-amplified in the DC mode ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SM preparations were fixed between a stationary stainless steel hook and an isometric force transducer (AE 801, SensoNor A/S, Norten, Norway) coupled to an AD converter Lab-Trax 4/16 (World Precision Instruments, Inc., Sarasota, FL, USA). The data were continuously recorded using DataTrax2 (World Precision Instruments ...
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bioRxiv - Plant Biology 2022Quote: ... in 50 nl water or with 50 nl water alone as a control using a glass capillary attached to a semi-automated micro-injector (World Precision Instruments, Inc. Sarasota, FL, USA) according to Aguero et al. ...