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Citations for World Precision Instruments :
601 - 650 of 702 citations for BODIPY FL DHPE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... in physiological saline containing 0.02% ascorbic acid or physiological saline (containing 0.02% ascorbic acid) was drawn into a 10 µL syringe with a 33 g needle (World Precision Instruments, Sarasota, FL, USA). 3 µL were infused into the medial forebrain bundle (coordinates ...
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bioRxiv - Cell Biology 2021Quote: ... Transendothelial electrical resistance (TER) was measured daily using an EVOM volt ohm meter equipped with STX-2 electrodes (World Precision Instruments, Sarasota, FL, USA) and electrical resistance calculated as previously described (Bartosova et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Whole cell voltage and current-clamp recordings were performed at 32–34 °C with electrodes (3–5MΩ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with intracellular solution of the following composition (in mM) ...
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bioRxiv - Cell Biology 2020Quote: ... The resistance between the upper and lower surfaces of IEC monolayers was measured before (Day 4) and after 2 days of stimulation (Day 6) using an EVOM2 epithelial Volt/Ohm meter (World Precision Instruments, FL, USA). The resistance of IEC monolayers was corrected by subtracting the resistance of scaffolds without cells and normalized by multiplying the effective scaffold surface area to provide a trans-epithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2021Quote: ... US were delivered by a MATLAB code-controlled custom-made gustometer consisting of two high pressure single syringe pumps (AL-1000HP, World Precision Instruments, Saratoga, FL) operating 50 ml Luer lock syringes ...
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bioRxiv - Neuroscience 2020Quote: ... conjugated with Alexa Fluor-555 (1 mg/ml) was injected into the vitreous of the right eye of Tbr2TauGFP/+ mice using a 35-gauge NanoFil system (World Precision Instruments, Sarasota, FL). Two days after CTB injection ...
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bioRxiv - Neuroscience 2020Quote: ... or ACSF (500 nl) were delivered unilaterally through a pulled glass pipette at a rate of 100 nl/min using an UltraMicroPump (World Precision Instruments, Sarasota, FL). Injection coordinates for mPFC ...
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bioRxiv - Neuroscience 2020Quote: ... Virus was delivered at a rate of 100 nl/min for up to 150-200 nl using an UltraMicroPump (World Precision Instruments, Sarasota, FL). Medial prefrontal cortex (mPFC ...
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bioRxiv - Neuroscience 2020Quote: ... AAVs or retrobeads were delivered unilaterally through a pulled glass pipette at a rate of 100 nl/min using an UltraMicroPump (World Precision Instruments, Sarasota, FL). Injection coordinates for VTA ...
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bioRxiv - Biophysics 2021Quote: ... a custom-made environmental chamber was built around the microscope and thermally-controlled at 37°C with a closed-loop Air-Therm heater (World Precision Instruments, Sarasota, FL). Samples in 48-well glass-bottomed plates were secured using a well plate holder (Ti-SH-W ...
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bioRxiv - Neuroscience 2020Quote: ... the fluorescent signal from the Alexa Floura 594 was used as a guide to visually place a borosilicate theta stimulating pipette (World Precision Instruments, Sarasota, FL) filled with aCSF in close proximity (~5 μm ...
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bioRxiv - Cell Biology 2022Quote: ... A block was then covered with an evenly spread layer of uncured PDMS (mixed at the base to cross-linking agent ratio of 10:1 (w/w)) and pressed against a No.1.5 glass-bottom dish (FD35-100, WPI Inc., Sarasota County, FL, USA). The dish with the negative mold was cured at 60°C for 2 hours to form the micropost topology on the glass surface.
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bioRxiv - Bioengineering 2020Quote: ... The lens surface was protected using a custom-made plastic cap made from the bottom tip of a microcentrifuge tube attached with Kwik-Cast (World Precision Instruments, Sarasota, FL). The same post-operative approach from the previous section was followed.
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bioRxiv - Physiology 2020Quote: ... TEP was measured by impaling the tubule lumen with a sharp microelectrode pulled from double-barreled theta-glass (World Precision Instruments, Inc. FL, USA) with reference to the basolateral bath ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell culture media was changed every 2 days for approximately 10 days until a stable monolayer was established.[32] To assess monolayer integrity TEER was measured across T84 monolayers with a voltohmeter (World Precision Instruments, Sarasota, FL). The monolayer was considered mature when the TEER of the monolayer reached a stable value of approximately 1000 ohms.cm2 ...
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bioRxiv - Bioengineering 2021Quote: The final step for finishing the probe was to solder ground and reference wires (Teflon Coated Silver Wire #AGT05100, World Precision Instruments, Sarasota, FL) to the probes ...
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bioRxiv - Neuroscience 2021Quote: ... 150 nL of AAV solution was injected bilaterally at each of the two depths (300 nL total/side) at a speed of 100 nl/min using a Nanoliter 2010 microinjector (World Precision Instruments, Sarasota, FL) fitted with glass pipettes with ~30-60 μm diameter tips ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The signals were filtered between 0.1 Hz – 100 Hz and amplified at x100 gain (AC) using a differential amplifier (DAM50, World Precision Instruments Inc., FL, USA).
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bioRxiv - Bioengineering 2021Quote: ... We simultaneously monitored bladder pressure at 1 kHz through the urethral catheter with a pressure transducer (DPT-100, Utah Medical Products, Midvale, UT) and analog amplifier (SYS-TBM4M, World Precision Instruments, Sarasota, FL).
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bioRxiv - Bioengineering 2021Quote: ... New Jersey) and custom 3D-printed components was assembled around the animal to support a 3-axis micromanipulator (502600, World Precision Instruments, Sarasota, FL) and linear actuator (M-235.5DD ...
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bioRxiv - Neuroscience 2023Quote: ... The right femoral artery was cannulated for continuous recording of mean arterial blood pressure (MAP) and heart rate (fH) using a BP-1 system (WPI Inc., Sarasota, FL, USA) and for monitoring of arterial blood pH ...
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bioRxiv - Cell Biology 2023Quote: ... It was measured by means of a volt-ohm meter (EVOM) equipped with a 24 mm EndOhm chamber (both from World Precision Instruments, Sarasota, FL). Resistance values for each condition were determined from a minimum of three individual cultures and corrected for the inherent cell culture insert resistance within five minutes after removing the plates from the incubator ...
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bioRxiv - Neuroscience 2023Quote: ... or hemoglobin constituted in aCSF at a 150 mg/mL concentration was injected at a rate of 8000 nL/min using a micro-infusion pump (World Precision Instruments, Sarasota, FL) to create the aCSF control and IVH-PHH conditions respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Infusions were made at a rate of 100 nL/min utilizing 29 Ga microinjection needles connected to FEP tubing secured to a 2 µl Hamilton syringe and a microinjection pump (UMP3, World Precision Instruments, Sarasota, FL). The infusion system was filled with distilled water and separated from the infused virus or drug by a small air bubble ...
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bioRxiv - Neuroscience 2022Quote: ... with a Gamry 600+ potentiostat (Gamry Instruments, Warminster, PA) (N=2 arrays) using previously published methods89.Silver ground and reference wires (AGT05100, World Precision Instruments, Sarasota, FL) were soldered to the PCB ...
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bioRxiv - Neuroscience 2022Quote: ... Gentle pressure was applied to the cover glass while the space between the coverslip and the remaining skull was filled with Kwik-sil adhesive (World Precision Instruments, FL, USA). A layer of vet-bond tissue adhesive (3M ...
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bioRxiv - Cell Biology 2022Quote: ... Confluency of the cultures were confirmed with trans-epithelial electrical resistance (TEER) using STX2 chopstick electrodes connected to an EVOM2 voltmeter (World Precision Instruments, FL, USA). The apical surface of the culture was washed with PBS once a week to clear mucus.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at least 3 days before the day of injection and the craniotomies were covered with silicone elastomer (Kwik-Cast, World Precision Instruments, FL, USA). Before injection ...
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bioRxiv - Neuroscience 2024Quote: ... the stock solution was further diluted to 0.6 mM with saline and 2 µl of the diluted solution was intracerebroventricularly administered after the control recording sessions using a NANOFIL micro-syringe (World Precision Instruments; FL, U.S.A.) and ultra-micro pomp (UMP3T-1 ...
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bioRxiv - Neuroscience 2024Quote: ... The area inside the chamber was washed with sterile saline every day for 7–10 days postoperatively to reduce reactive tissue formation around the electrode arrays and then filled with silicone polymer (Kwik-Cast, World Precision Instruments, Sarasota, FL).
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bioRxiv - Neuroscience 2024Quote: ... cut to a length of 9.0 cm with a slight taper on one end with a suture bead made from medical grade silicone adhesive (Kwik-sil, World Precision Instruments, Sarasota, FL) placed 2.8 cm from the tapered side for secure anchoring ...
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bioRxiv - Neuroscience 2023Quote: ... Each electrode was coated with a conductive paste (SignaGel; Parker Labs Fairfield, New Jersey) and attached to the intact scalp with a biocompatible silicon elastomere (KwikSil; World Precision Instruments, Sarasota, FL]). Electrode impedances were typically 1-2 kOhms and never exceeded 15 kOhms.
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bioRxiv - Physiology 2023Quote: ... Cell monolayer transepithelial voltage (VTE) and resistance (RTE) were measured using an epithelial volt-ohmmeter equipped with stick electrodes (World Precision Instruments, Sarasota, FL). The equivalent transepithelial current (ITE ...
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bioRxiv - Neuroscience 2023Quote: ... 15nm Aurovist (0.1 mg/μL) or 1x PBS was then continuously infused into the lateral cerebral ventricle using a syringe pump (World Precision Instruments, Sarasota, FL) via a Hamilton syringe (Hamilton Company ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: ... back region of the neck shaved and placed in a stereotaxic instrument (David Kopf instruments, CA, USA) with a face mask (World Precision Instruments, FL, USA). Head was inclined ∼70 degrees forward and a longitudinal incision was made in the skin at the level of the C1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Oocytes were injected with 27.6 nL (27.6 ng of each cRNA) of RNA using the Nanoliter 2010 injector (World Precision Instruments, Inc., Sarasota, FL, USA). Odorant-induced currents of oocytes expressing functional odorant receptor complexes were recorded using the two-microelectrode voltage-clamp technique (TEVC) ...
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bioRxiv - Microbiology 2022Quote: ... Cilia activity was checked every 2~3 days and trans-epithelial electrical resistance (TEER) was measured to ensure confluency of the cells using the EVOM meter (World Precision Instruments, Sarasota, FL).
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bioRxiv - Neuroscience 2022Quote: A pair of platinum wire electrode with a ball-shaped round tip were placed epidurally on top of the exposed side of dorsal column and connected to a stimulation isolator (A385 High Current Stimulus Isolator, World Precision Instruments, Sarasota, FL). The isolator received trigger inputs from a pulse stimulator (Model 2100 ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (3-6 MΩ) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Biophysics 2022Quote: ... The scutum mount was rigidly attached to a 10 N force transducer with a resonant frequency of 300 Hz (FORT1000, World Precision Instruments, Sarasota FL), and the posterior phragma mount was rigidly attached to the shaker head ...
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bioRxiv - Neuroscience 2023Quote: ... the vagus nerve was mechanically stabilized on the micro-cuff electrode by applying a drop of 2-component silicone (Kwik-Sil, World Precision Instruments, Sarasota, FL) and then sectioned either caudal or rostral to the cuff ...
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bioRxiv - Bioengineering 2024Quote: Molds for the device assembly were fabricated using PDMS and biopsy punches of 4 mm and 7 mm diameters (World Precision Instruments, Sarasota, FL). A large well was made in the PDMS with the 7 mm punch ...
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bioRxiv - Physiology 2024Quote: ... Mouse temperature was monitored by rectal probe and maintained at 37°C through automated warming using a controlled warming pad (ATCC 2000, World Precision Instruments, Sarasota, FL). Anesthetic plane was assessed by respiratory rate and toe pinch reflex ...
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bioRxiv - Neuroscience 2024Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... 5 electrical pulses at 20 Hz (duration: 500 μs; intensity: 20–80 μA) every 40 seconds were generated by a stimulus isolator (A385, World Precision Instruments, FL, USA) and delivered through a bipolar tungsten electrode (FHC ...
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bioRxiv - Molecular Biology 2024Quote: ... The electrical resistance of cell monolayers was measured on day 4 (before stimulation) and day 7 (after 3 days of stimulation) using an EVOM2 epithelial Volt/ Ohm meter (World Precision Instruments, FL, USA). The resistance of cell monolayers was corrected by subtracting the resistance of wells without cells and normalized by multiplying by the effective well surface area to provide transepithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2024Quote: Just prior to noise exposure a single ear of each animal was protected by filling the ear canal with a silicone elastomer (Kwik Seal, World Precision Instruments, Sarasota, FL). Awake freely moving animals were exposed to a 16 kHz tone ...
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bioRxiv - Cell Biology 2024Quote: ... pH of the media during the assays did not change and was confirmed using glass tipped pH microelectrodes (World Precision Instruments, Sarasota Fl, USA). Indo-1 stained cells were added into the microtiter plate at unform cell density across the plate and fluorescence (340/400:475 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... were inoculated 2.35 mm below the cortical surface using a 10 µL Hamilton syringe (Hamilton, Reno NV, USA) connected to an automatic push-pull microinjector (WPI SmartouchTM, Sarasota FL, USA), by advancing the 26G needle 3.85 mm from the surface of the skull (∼1mm skull-to-brain surface distance) ...