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Citations for New England Biolabs :
401 - 450 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified using a PCR purification kit (NEB) following manufacturer instructions.
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bioRxiv - Genomics 2023Quote: ... Amplicons were purified with the Monarch PCR kit (NEB), digested with MfeI-HF and SphI-HF (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... and purified with Monarch PCR & DNA cleanup kit (NEB). PURExpress in vitro translation reaction was assembled in RNase-free tubes according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and purification with a PCR clean-up kit (NEB) following the manufacturer’s protocols ...
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bioRxiv - Biophysics 2024Quote: ... and Monarch PCR&DNA Cleanup Kit (5 μg) (NEB). The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Reactions were purified with a PCR purification kit (NEB). Approximately 120–150 ng of DNA was used as a template for a T7 in vitro transcription (IVT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was column-purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs). The IVT reaction including DNAseI digest was performed with the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: PCR products were extracted with Monarch PCR & DNA Gel Cleanup Kit (New England Biolabs, Ipswich, MA) and sequenced by ABI 3730 automated DNA sequencer.
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bioRxiv - Neuroscience 2021Quote: ... Kv1.2wt-T2A and Kir2.1wt-T2A fragments were magnified by PCR (Phusion High-Fidelity PCR Kit, NEB) from pcDNA3-Kv1.2wt (human cDNA template ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were generated from genomic DNA with a Phusion® High-Fidelity PCR kit (NEB) and primers that introduced KpnI and XhoI restriction sites ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed with a Phusion High-Fidelity PCR Kit (New England Biolabs, Ipswich, MA, USA) in 20µL reactions mixed with 2µL cDNA and 10µM primers ...
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bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using NEBMonarch PCR and DNA Clean-up Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (NEB, T1030) and used as “megaprimers” that are denatured and annealed to the original plasmid (pNG93 ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using the Monarch® PCR & DNA clean-up kit (New England Biolabs). In vitro expression was performed for 2 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Biochemistry 2021Quote: ... libraries were generated using 5 μl of the purified RT reaction product and 4-8 cycles of PCR with Q5 high fidelity polymerase (NEB, M0491S). PCR reaction products were column purified ...
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bioRxiv - Bioengineering 2023Quote: ... Specific donor sequences for small edits were encoded in primers and substituted into the RT-DNA-encoding region of the ncRNA with a PCR and KLD reaction (NEB M0554). Donor sequences for larger insertions were cloned through Gibson assembly ...
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bioRxiv - Microbiology 2023Quote: ... or treated with 0.1 mM carbonic anhydrase inhibitor – S4 for 24 h was subjected to semi-quantitative RT-PCR analyses using Q5 high-fidelity DNA polymerase (New England Biolabs Inc.) according to manufacturer’s protocol in a T100 Thermal cycler (BIO-RAD) ...
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bioRxiv - Cell Biology 2019Quote: ... and detected using components based on the Phototope-Star detection kit (New England BioLabs, Cat# N7020). All T ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse transcriptase (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesis was carried out using LunaScript™ RT SuperMix Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: One μg of RNA was reverse transcribed using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manual ...
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bioRxiv - Molecular Biology 2022Quote: ... subsequently used to generate cDNA with LunaScript RT SuperMix Kit (New England Biolabs, catalog no. E3010S). Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... reverse transcription from mRNA was performed using the LunaScript® RT SuperMix Kit (New England BioLabs) following standard protocols ...
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bioRxiv - Immunology 2023Quote: Reverse transcriptase qPCR was performed using Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006L). The following predesigned primers and probe sets for each target and the housekeeping gene GAPDH were purchased from IDT:
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bioRxiv - Molecular Biology 2023Quote: ... RT-LAMP reactions were performed using the WarmStart® LAMP Kit (New England Biolabs Cat # E1700) following the manufacturer’s instructions without addition of the fluorescent dye ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Physiology 2023Quote: ... Samples were reversed transcribed using the LunaScript RT SuperMix Kit (New England BioLabs, Ipswich, Massachusetts, USA) and relative gene expression was quantified via CFX-96 Real-Time PCR Detection System (RRID:SCR_018064 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Neuroscience 2020Quote: ... Tough decoy for miR-124 was synthesized (GeneScript) and subcloned into pLemir vector using MluI (NEB, R0198) and NotI sites.
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR reagents (NEB, E3005) and the primers listed in Table S3.
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bioRxiv - Microbiology 2020Quote: ... and Protoscript II RT (NEB). 10 μl of cDNA was PCR amplified for 18 cycles with Illumina TruSeq primers and Phusion DNA polymerase ...
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bioRxiv - Cell Biology 2023Quote: ... Luna WarmStart RT (NEB, M3002) was used for primer extension at 55 ⁰C ...
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bioRxiv - Microbiology 2019Quote: KLRC2 genotypes were determined by touchdown PCR using the Phusion High Fidelity PCR kit (New England Biolabs) using previously described methods40 ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... PCR was performed according to manufacturer’s protocol (Phusion High-Fidelity PCR kit, New England Biolabs, Ipswich, MA).
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bioRxiv - Genetics 2021Quote: ... The A-tailed PCR products were cleaned with Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB) and ligated into pGEM®-T Easy vector (Promega ...
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bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products with the expected size were column-purified with the Monarch PCR & DNA Cleanup Kit (NEB) and Sanger sequenced by GATC-Eurofins or Microsynth ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed using the Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, UK) and the final reaction contained 250 ng of template DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Eluted DNA was enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...