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Citations for New England Biolabs :
501 - 550 of 6948 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The final cDNA sample was then split into 16 separate reactions for final PCR amplification using 16 unique Illumina indexing primers and Q5 Hot Start High-Fidelity 2X Master Mix (NEB). After amplifications ...
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bioRxiv - Microbiology 2024Quote: ... The relevant regions were amplified by PCR using primers designed for this study and shown in Table S1 Q5 High Fidelity DNA polymerase (NEB) was used for sequence analysis with appropriate purity and accuracy.
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bioRxiv - Developmental Biology 2024Quote: ... both CRISPRi plasmid pools and genomic DNAs were PCR amplified with Miseq_Adaptor primers (Table S5) using Q5 High-Fidelity DNA Polymerase (NEB; M0491L). PCR samples were run on a 2% agarose gel and purified using a Gel Extraction Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated by amplifying sgRNA sequences from genomic DNA using bar-coded Illumina-compatible adapter-containing primers and NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were pooled and purified with a ZymoSpin V column with Reservoir (Zymo Research) ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were genotyped by sequencing PCR products using primer vangl2 fwd 5’-ATTCCCTGGAGCCCTGCGGGAC-3’ and primer vangl2 rev 5’-AGCGCGTCCACCAGCGACACAGC-3’ or restriction digest of the PCR products with Alu1 (R0137S, NEB). The vangl2 wild type allele stayed intact while the vangl2vu67 mutant allele was identified by a digested PCR product.
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bioRxiv - Biochemistry 2020Quote: ... small RNA library preparation was performed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) (NEB). cDNA libraries were sequenced on a HiSeq2000 platform in a spike-in format in paired-end 50 (PE50 ...
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bioRxiv - Microbiology 2019Quote: High throughput sequencing libraries were prepared using RNA isolated from PAR-CLIP samples using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2; cat# E7580, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: High throughput sequencing libraries were prepared using RNA isolated from total RNA or PAR-CLIP samples using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2; cat# E7580, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... One-tenth of the RT reaction was used as a template for real-time PCR using Luna Universal qPCR Master Mix (New England Biolabs) on a QuantStudi 6 system ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of the cDNA was used as template for the RT-PCR reaction with OneTaq DNA Polymerase (New England Biolabs). PCRs were run at the following conditions ...
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Microbiology 2020Quote: RNA samples were confirmed to be DNA-free by conducting test PCRs on the 16S rRNA gene and converted to cDNA using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... The Luna Universal One-Step RT-qPCR kit (E3005L) was used for qRT-PCR analysis following the protocol described by the supplier (NEB) by using a CFX96 real-time C1000 thermal cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: The copy number of viral RNAs were titrated by qRT-PCR using Luna Universal Probe One-Step RT-qPCR Kit (NEB) and LightCycler 96 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Molecular Biology 2021Quote: ... tropicalis pseudouridine synthase Pus7 was amplified from total RNA using the OneTaq One-step RT-PCR kit (New England Biolabs). XmaI and XhoI restriction sites were added with oligonucleotides and the resulting fragment was cloned into the p415Gal1 vector.
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bioRxiv - Molecular Biology 2022Quote: ... aeruginosa AZPAE12409 was inserted into pBL1MBPG2L4 RT-8XHis by PCR amplifying a 658-bp region of genomic DNA containing the Direct Repeats with Gibson forward and reverse primers that append flanking KpnI sites and inserting the KpnI-digested PCR product into the KpnI site of pBL1-MBPG2L4 RT-8XHis by using NEBuilder HiFi DNA Assembly (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and rps10 (ENSDARG00000034897) were amplified by RT- PCR and cloned into pCS2+HA using NEBuilder HiFi DNA Assembly (New England BioLabs). The pCS2+HA was generated by inserting synthetic DNA fragments with HA- tag sequence into pCS2+ via EcoRI/XbaI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were then subjected to qRT-PCR of BB0838 and flaB transcripts using the Luna Universal One-Step RT-qPCR kit (NEB) and an ABI Prism 7500 system (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... The Ct values of samples were determined by quantitative PCR (qPCR) using a Luna Universal Probe One-Step RT-qPCR Kit (cat. #E3006, New England Biolabs) with LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Genetics 2023Quote: ... The presence of infectious rIBV in the allantoic fluid was confirmed by a two-step reverse transcription polymerase chain reaction (RT-PCR) protocol using Protoscript II reverse transcriptase (NEB) and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Two μg of RNA served as template for cDNA synthesis using oligo dT and murine leukemia reverse transcriptase as implemented in the OneTaq RT PCR kit (New England Biolabs). A panel of oligonucleotides designed to amplify mouse Ig V genes as described by Wang et al (81 ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 96-well plate and qPCR was performed in a LightCycler 96 System (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 100 ng of total RNA was used in the detection and quantification of TgCPDH transcripts using the Luna Universal One-Step RT-PCR kit (NEB). Data acquisition was collected using the BioRad CFX96 Touch Real-Time PCR detection system ...
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bioRxiv - Cell Biology 2023Quote: ... AMV RT (NEB, M0277L) was used for primer extension at 42 ⁰C ...
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bioRxiv - Genetics 2024Quote: ... 10x RT buffer (NEB) and 10mM dNTP mix (NEB) ...
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bioRxiv - Microbiology 2022Quote: NEB buffer set (New England Biolabs, catalog #: B7200S).
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bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
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bioRxiv - Developmental Biology 2019Quote: ... and the resulting fragment was restriction digested and cloned into pME-miR using T4 ligation (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the piggy-bac vector pPBhCMV1-miR(BsgI)-pA-3 was digested with BsgI (#R05559S, NEB) and the digested vector excised from a DNA agarose gel and the DNA purified ...
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bioRxiv - Microbiology 2020Quote: ... the genes were PCR-amplified using primers containing cut sites for the restriction enzymes EcoRI (forward) and SalI (reverse) (New England Biolabs, NEB, Australia) for gtr29 ...
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bioRxiv - Microbiology 2021Quote: ... A screen for clones containing an insert into pBAD33 was carried out using 1 μl of this suspension as a PCR template using primers oMJD204 and oMJD205 and OneTaq Quickload 2X Master Mix (NEB, #M0486S), according to the manufacturer’s instructions (annealing temperature 45 °C ...
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bioRxiv - Neuroscience 2022Quote: ... donor plasmids were constructed by generating fragments using PCR from the indicated template with indicated primers in Data File 1 and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S):
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bioRxiv - Genomics 2020Quote: ... was amplified as per the ChIPmentation protocol [64] using indexed and non-indexed primers and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) in a thermomixer with the following program ...
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bioRxiv - Cell Biology 2019Quote: ... Homologous 15bp overhangs on the 3’ end of the inverse PCR primers were ligated following the NEB T4 Ligation protocol (New England Biolabs # M0202S) to introduce the 5AA sequence ...
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bioRxiv - Biochemistry 2019Quote: ... Three replicates of each condition were subjected to fluorescent PCR with a FAM-labelled forward primer (below) and with Phusion polymerase (NEB #M0530S) for 32 cycles ...
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bioRxiv - Immunology 2020Quote: ... Target DNA was amplified from a cDNA template or existing plasmids using primers and PCR conditions shown in Tables S2-S4 using Q5 High-Fidelity 2X Master Mix (New England Biolabs # M0494L). Primers were ordered with 5’ base extensions that overlapped expression vectors on either side of the restriction sites ...
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bioRxiv - Genetics 2019Quote: ... PCR was performed using the designed primers (Table S4) on converted cDNA using NEB taq polymerase (New England Biolabs, Ipswich, MA), and run on a 2% agarose gel with ethidium bromide to test for specificity.
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bioRxiv - Genetics 2021Quote: ... At first, PCR were run with primers (oJZ_241/242, see S3 File) with LongAmp polymerase (New England Biolabs; Ipswich, MA, USA), purified and sequenced (Sanger sequencing?) ...
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bioRxiv - Plant Biology 2020Quote: ... the sgRNA backbone and either a U6-29 promoter or a tRNA buffer were produced by four-primer PCR (described in Xing & al., 2014) with a proofreading enzyme (Q5 polymerase, New England Biolabs, M0491). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were inserted into PCR-amplified (primers 17-18 in Table S3) and purified pRSET vector fragment by Gibson Assembly (NEB, USA) to add sequence parts necessary for cDNA display generation to the DNA library ...
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bioRxiv - Neuroscience 2020Quote: ... fragments were generated by PCR from the indicated template with the indicated primers (Data File 1) and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S). Plasmids were transformed into NEB competent cells (NEB C2987I) ...
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bioRxiv - Neuroscience 2020Quote: ... we generated DNA template for transcription of a new batch sgRNA (separate from that used for validation) by annealing two partially overlapping PCR primers (IDT, PAGE purified) and extending with NEBNext High-Fidelity polymerase (NEB, M0541S). We then transcribed each sgRNA in vitro using the HiScribe T7 Kit (NEB ...
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bioRxiv - Systems Biology 2021Quote: ... The final enrichment PCR used primers MO588 and MO589 (Supplementary file 6) for 20 cycles at an annealing temperature of 66C (NEB Phusion), followed by purification with the Monarch PCR kit ...
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bioRxiv - Plant Biology 2021Quote: The coding sequence of NKS1 with or without stop codon was amplified by PCR using gene specific primers (Table S7) from cDNA using Phusion High-Fidelity DNA polymerase (F530S, NEW ENGLAND BioLabs, Inc) or PrimeSTAR HS DNA polymerase (R010A ...
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bioRxiv - Microbiology 2021Quote: ... The second-round PCR was performed for 30 cycles with Illumina flowcell adaptor primers using Phusion® High-Fidelity DNA Polymerase (NEB): 98 °C 5 min - (98 °C 30 sec ...
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...