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Citations for New England Biolabs :
501 - 550 of 2731 citations for Who Coplanar & Mono Ortho Pcb + Pcb 170 & Pcb 180 13C12 99% 20 Ng Ml In N Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 4 ng gBlock template and 1 Unit Phusion High-Fidelity DNA polymerase (NEB). The reaction was subjected to a 2-minute initial denaturation at 98 °C ...
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bioRxiv - Genomics 2023Quote: ... 100 ng of gDNA was incubated with dsDNA Fragmentase (New England Biolabs, USA) at 37 °C for 35 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... The restriction digest was set up directly afterwards and performed overnight at 37°C with agitation (750 r.p.m.) with HindIII (NEB; 20 µl of 20 U/µl). Using biotin-14-dCTP (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 U EcoRI-HF (cat. no. R3101S, New England BioLabs), and ultrapure water ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 20 U RNase inhibitor (M0314L, NEB) for 60 min at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Genomics 2019Quote: ... 20 U of the restriction enzyme BstXI (New England Biolabs) were added directly to the amplification reaction and the mixture was incubated for 3 hours at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... NaCl 20 mM) with 200 μM of each dNTPs (NEB), 0.1% of Bst 2.0 WarmStart® DNA Polymerase (8,000 U/ml) ...
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bioRxiv - Microbiology 2021Quote: ... 2 µl of 20 µM SpCas9 (NEB, Cat. No. M0646M) was placed in the bottom of a 0.25 ml PCR tube ...
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bioRxiv - Microbiology 2021Quote: ... Each 20 uL reaction contained 1X Thermopol Reaction Buffer (NEB), 125uM dNTPs ...
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bioRxiv - Microbiology 2019Quote: ... 20 units of EcoRI-HF® (New England Biolabs®) was mixed with 500 ng of isolated DNA (40 units/µg DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μL DpnI enzyme (20 U, New England Biolabs #R0176L) was added to the 25 μL PCR mixture immediately after the final extension and incubated at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µl of the ThermoPol Buffer (New England Biolabs, # B9004S) was added to the samples and boiled for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... O-glycosidase (New England Biolabs, 20 000 U/μg protein), α2-3,6,8 neuraminidase (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... 20–27 and the NEBuilder Hifi DNA Assembly kit (NEB).
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bioRxiv - Biophysics 2019Quote: ... with 2 μl of 20 mM BG-GLA-NHS (NEB) in 20 μl 100 mM sodium borate buffer ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.5 μL (20 U) of RNase inhibitor (New England Biolabs), and 2.5 μL of TGIRT-III reverse transcriptase (InGex ...
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bioRxiv - Cell Biology 2020Quote: ... was added and incubated with 20 units ExoI nuclease (NEB) for 1 h at 37°C before purification with 42 μl AMPure beads (Beckman Coulter) ...
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bioRxiv - Bioengineering 2021Quote: ... 4.25 μl EnGen Cas9-NLS (20 μM, New England Biolabs), 2.7 μg sgRNA at 37°C for 10 min.
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... 20 U of RNase murine inhibitor (NEB, Ipswich, MA, USA), 0.15 µL Cas13a endonuclease (25 nM ...
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bioRxiv - Genomics 2022Quote: ... and resolved on a 4–20% SDS-polyacrylamide gel (NEB). The presence of MeCP2 was assayed by western blotting using anti-MeCP2 monoclonal antibody M6818 (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... 5% (v/v) BsaI at 20 U/μL (NEB #R3733), 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µL NEBNext Quick Ligation Reaction Buffer 5X (NEB, B6058S) and 10 µL Quick T4 DNA Ligase (NEB ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we performed 2 reactions per sample (20 µl 5× NEB Q5 buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µL PCR reaction contained 1× OneTaq Master Mix (NEB), 0.2 µM of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 20 µl of Proteinase K (NEB) and another incubation at 65°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... or CatL-cleaved GPs were incubated with Protein N– glycosidase F (PNGaseF, 250U; New England Biolabs, Ipswich, MA) under reducing conditions for 16 h at 37℃to remove N–linked glycans ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of ProtoScript II reverse transcriptase 200 U / µL (cat. n° M0314L NEB, New England Biolabs, USA), 2 µL of random primer mix 60 µM (cat ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of ProtoScript II reverse transcriptase 200 U / µL (cat. n° M0314L NEB, New England Biolabs, USA), 2 µL of random primer mix 60 µM (cat ...
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bioRxiv - Genetics 2022Quote: ... N is for a random nucleotide) were ligated to the small RNA by T4 RNA ligase 2 (NEB) and T4 ligase 1 (NEB ...
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bioRxiv - Biophysics 2021Quote: ... of 0.1M sodium hydroxide: 0.02M 2-(n-morpholino) ethanesulfonic acid (MES) or 1X NEB DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Neuroscience 2021Quote: ... Phosphatase treated samples were enzymatically treated for 3 n at 37°C shaking (1.25 μl 10 μM MnCl2, 1.25 μl 20X NEB buffer ...
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bioRxiv - Molecular Biology 2022Quote: The recombinant pET6xHN-N constructs were transformed into Escherichia coli strain BL21(DE3) (New England Biolabs, MA, USA). The transformed clones were cultured at 37 °C in LB medium with 100 μg/mL Carbenicillin and were induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: Removal of N-linked oligosaccharides in mini-procollagens was performed with PNGase F (New England BioLabs, glycerol-free). Ten micrograms of mini-procollagens were first denatured at 100 °C for 10 min in Glycoprotein Denaturing Buffer provided with the enzyme ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... using an RNA input of 100 ng per sample (New England BioLabs, Ipswich, USA). Subsequently ...
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bioRxiv - Genetics 2021Quote: ... About 10 ng of genomic DNA was fragmented using the NEBNext dsDNA fragmentase (NEB) and sequencing libraries were generated using the NEBNext Ultra DNA Library prep kit (NEB ...
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bioRxiv - Genetics 2021Quote: ... After digesting 100 ng of DNA from each sample using EcoRI and MseI (NEB), adapters containing barcode sequences were ligated to the ends of the DNA fragments ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 500 ng DNA were digested with 10 units of CviQI (NEB, cutting site G’TAC) at 25°C for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... and 100 ng of the sgRNA in NEBuffer 3.1 (New England Biolabs, Ipswich, USA) for 20 minutes at 37°C and 10 minutes at 65°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we digested 1,000 ng of genomic DNA with EcoRI and MspI (New England Biolabs) and cleaned the digested DNA using Agencourt AMPure XP beads (Beckman Coulter Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of genomic DNA was treated with McrBC enzyme (New England Biolabs, M0272L) at 37°C overnight and then for 10 min at 75°C to deactivate the enzyme ...
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bioRxiv - Biochemistry 2020Quote: ... 10-50 ng of mononucleosomal DNA was incubated with 1.25 Units Taq polymerase (NEB), 3.75 Units T4 DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of genomic DNA was digested with MspI+EcoRI and HpaII+EcoRI (NEB) in parallel reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 ng PCR product was incubated with 2 µL 10X NEBuffer 2 (NEB, B7002S) and nuclease-free water adding up to 19 µL using the following program ...
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bioRxiv - Genomics 2022Quote: 500 ng to 1 μg gDNA were cleaved by SacI-HF (New England Biolabs) for 15 min at 37 °C ...