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Citations for New England Biolabs :
551 - 600 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Variants were transformed into BL21(DE3) cells (NEB), a single clone was picked and grown to saturation in LB media supplemented with kanamycin (50 µg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated in 2i medium containing 300 nM SNAP-Cell 647-SiR (New England Biolabs, Ipswich, MA, USA, S9102S) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: The cells were infected with ZIKV and DENV2 and lysed using the Luna Cell Ready Lysis reagent (New England Biolabs, UK). Quantification of viral RNA was performed from cell lysates using the virus specific primers and their levels were normalized to mRNA of GAPDH (Table 1) ...
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bioRxiv - Bioengineering 2023Quote: Samples were resuspended in 5 µl 1X NEBNext Cell Lysis Buffer of the NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB, E6420). After 5 min incubation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... RNA from Luminal mature and progenitor cells was amplified using the “NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification” (NEB). For RNA-Seq 20,0000 to 50,000 sorted basal and luminal cells from the shTrps1 ubiquitous mouse model (Fig S4 ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Cell Biology 2020Quote: ... For SNAP-Cell 647-SiR (New England Biolabs, Inc.) dye labeling ...
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bioRxiv - Genetics 2021Quote: ... After transformation with BL21 (DE3) competent cells (NEB, C25271), overnight cultures were inoculated in LK media and incubated at 37°C until the OD600 approached ∼0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... or SNAP-Cell 647-SiR (New England Biolabs, S9102S) according to manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: ... we transformed it into 10beta electrocom petent cells (NEB), and obtained 1.39 million unique transformants.
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bioRxiv - Molecular Biology 2022Quote: Cell extracts were prepared in RIPA lysis buffer (NEB) supplemented with protease inhibitors (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21 DE3 cells (New England Biolabs, Ipswich, MA). Cells were grown in LB media (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Molecular Biology 2021Quote: A single colony of BL21DE competent cells (NEB, C2527H), transformed with PltB (or its mutant-PltB S35A ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice in 1× NEBuffer3.1 (NEB, B7003S) and resuspended in 342 μl of 1× NEBuffer3.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... with competent BL21 (DE3) and DH5α cells (NEB, USA). pGAD-T7 Rec (Clontech ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21(DE3) cells (New England Biolabs, Ipswich, MA) and expressed and purified as previously described for SP (REF) ...
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bioRxiv - Molecular Biology 2019Quote: ... All restriction enzymes and NEB5α competent cells (NEB C2987H) are from NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3) chemically competent cells (NEB product # C2530H). The following day ...
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bioRxiv - Biochemistry 2020Quote: ... coli NiCo21 (DE3) cells (New England Biolabs, Ipswich, MA) grown in TBmod plus MOPS medium supplemented with 25μg/ml kanamycin and no biotin were used ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-myc (NEB/Cell Signaling, 2276; 1:1500) o/n at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transformed into NEB competent cells (NEB C2987I), purified with the NucleoBond Xtra Midi Endotoxin Free kit (Clon-tech 740420.50) ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP Cell-SIR647 (New England BioLabs, S9102S, 0.3 µM); PEG 1500 (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with RNaseH (New England Biolabs, M0297S) for 2 hours ...
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bioRxiv - Synthetic Biology 2021Quote: Glycerol stocks of NiCo21(DE3) cells (New England Biolabs) transformed with each GFP variant were streaked onto LB agar plates with added ampicillin (100 μg/ml ...
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bioRxiv - Physiology 2020Quote: ... phospho-AKT (#9271, 1:1000, Cell signalling, NEB, UK), total-AKT (#9272 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were batch bound to amylose resin (NEB) for 2 hours to capture the 2XMBP tagged BRCA2 proteins ...
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bioRxiv - Cell Biology 2022Quote: ... cells were stained with SiR647-SNAP (New England Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2022Quote: ... Transformed cells (New England Biolabs E.coli Turbo Cat.No C2984H) made competent using the protocol provided in [Additional file 1] were placed in the incubator overnight at 37° and colonies were screened for desired mutants using BsaI-HF®v2 (New England Biolabs Cat.No R3733S).
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid was transformed into T7 express cells (NEB) according to manufacturer’s instructions and expressed in Luria-Bertani broth at 37 °C with shaking at 180 rpm before induction by IPTG at an optical density of 0.6 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were recovered in SOC outgrowth medium (NEB B9020S) for 1 hour at 37°C before selection on kanamycin plates ...
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bioRxiv - Microbiology 2020Quote: ... 1 µM SNAP-Cell TMR Star (New England Biolabs) was added to label the SNAP tag ...
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bioRxiv - Microbiology 2021Quote: ... coli C2987 chemically competent cells (New England Biolabs, Inc). pUC19::PBP7 was used as a template for Pfu-mediated deletion mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with helper phage M13KO7 (NEB N0315S) or VCSM13 (Agilent 200251 ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21(DE3) cells (New England Biolabs, cat#C2527I) following manufacturer recommendations and selected on LB+CA100 before maintenance as a 15% glycerol stock at −80°C ...
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bioRxiv - Genomics 2020Quote: ... into dam-/dcm-competent E.coli cells (NEB, Ipswich, MA). Unmethylated pUC19 was extracted using the Monarch Plasmid Miniprep Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were transformed into NEB5α competent cells (NEB C2987H) for Gibson Assembly or TOP10 competent cells (Thermo Fisher K204040 ...
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bioRxiv - Microbiology 2022Quote: NEB stable competent cells (New England Biolabs, catalog #: C3040H).
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bioRxiv - Cell Biology 2022Quote: ... fixed cells were incubated with λ-phosphatase (NEB, P0753S) for 2 hours at 30°C prior to immunostaining ...
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bioRxiv - Cell Biology 2020Quote: ... or electrocompetent DH5α cells (New England Biolabs, Cat# C2989) by electroporation ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21(DE3) chemically competent cells (NEB product # C2530H) were transformed with 100 ng of pET24a His8–MBP plasmid (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cell samples were deglycosylated using PNGase F (NEB P0708L) per the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... coli cells (DH5) carrying pMAL-c5x (New England Biolabs) or pMAL-NbERF-IX-33a were cultured overnight at 37°C in rich medium (1% tryptone ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ABCB1 (Cell Signaling via New England Biolabs, Frankfurt, Germany), p53 (Enzo Life Sciences ...
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bioRxiv - Synthetic Biology 2020Quote: ... Escherichia coli BL21 (DE3) cells (New England Biolabs, USA) were used for recombinant protein expression ...
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bioRxiv - Genetics 2020Quote: ... coli competent cells (10-beta Competent E.coli, NEB C3019I) through an incubation of 30 minutes on ice and a heat shock of 30 seconds at 42°C ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain NEB Express C2566 cells (New England Biolabs) carrying the pRare2 ...
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bioRxiv - Genomics 2019Quote: ... We then transformed 10-beta electrocompetent cells (NEB C3020) with the plasmids in duplicate following the manufacturer’s protocol ...