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Citations for New England Biolabs :
451 - 500 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Escherichia coli BL21(DE3) cells (NEB, USA) were transformed with pET-21b plasmid encoding for N-terminally His-tagged NanoLuc gene ...
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bioRxiv - Genetics 2024Quote: ... coli 10β cells (New England BioLabs, C3019I). Following heat-shock ...
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bioRxiv - Genetics 2024Quote: ... and transformed into DH5a cells (NEB C2987l). A single cysteine sequence was added to the linker between Met4 and MBP to facilitate maleimide conjugation of fluorophores for imaging ...
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bioRxiv - Systems Biology 2024Quote: ... was electroporated into 10beta electrocompetent cells (NEB). After recovery ...
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bioRxiv - Synthetic Biology 2024Quote: ... transformed into electrocompetent DH5α cells (NEB, C2989K), and plated on 2XYT-carbenicillin-1% glucose ...
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bioRxiv - Biophysics 2023Quote: Escherichia coli BL21 (DE3) cells (#C2527, NEB) were transformed with the appropriate plasmid and grown at 37°C to an OD600 of 0.8 - 1.0 in MOPS-buffered Terrific broth (TB) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL-21 cells (New England Biolabs) were used for the transformation of pET-28b(+ ...
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bioRxiv - Biochemistry 2024Quote: ... coli NEB C2566 cells (New England Biolabs). The cells were grown at 37°C in TB medium for HINT1 ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (New England BioLabs).
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bioRxiv - Biophysics 2024Quote: ... coli chemically-competent cells (New England BioLabs). Single colonies of transformed cells from agar plates containing 100 μg/mL ampicillin and grown at 37 °C were used for starter cultures using 50 mL LB media in 250 mL shake flasks containing 100 μg/mL ampicillin ...
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bioRxiv - Plant Biology 2020Quote: All recombinant CPK28 clones were transformed into lambda phosphatase-expressing BL21 (DE3) E.coli cells for the production of dephosphorylated proteins or into T7 Express cells (New England Biolabs) for production of hyperphosphorylated protein (53) ...
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bioRxiv - Cancer Biology 2020Quote: ... pre-existing histones were first quenched by incubating cells with 5 µM SNAP-cell Block (New England Biolabs; S9106S) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh medium containing 4 μM SNAP-Cell TMR-Star (New England Biolabs, Inc.) for 15 min at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were spun down and washed three times in ice cold PBS and the cells incubated with DpnI (NEB) to remove any residual plasmid contamination ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Genomics 2023Quote: ... cells in the latter plate were labeled with HaloTag Oregon Green and SNAP-Cell 647-SiR (New England Biolabs) simultaneously according to the manufacturers’ protocols ...
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bioRxiv - Genomics 2022Quote: ... OMNI ATAC-Seq was performed as previously described with 320,000 human CD4+ T cells (in total) in batches of 100,000 cells per reaction (NEB #M0544S).(26 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each library was split in two and transformed separately into 5-alpha cells (NEB, 3.5uL DNA in 35uL cells). Plasmid library isolation ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling with SNAP-Cell 647-SIR was done as described in the manual for SNAP-Cell 647-SIR (NEB). Afterward ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling with SNAP-Cell 647-SIR was done as described in the manual for SNAP-Cell 647-SIR (NEB) before imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were grown on glass coverslips and pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 µM of the non-fluorescent substrate SNAP-cell Block (New England Biolabs) for 30 min followed by a 30 min-wash in fresh medium and a 2 hr-chase ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNAs were isolated from Calu-3 infected cells 48h post infection using the Luna Cell Ready Lysis Module (New England Biolabs). Viral RNA were quantified by qRT-PCR in triplicate as described in [27] ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2019Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Dissociated cells were resuspended in 1ml of Cell Hashing Staining Buffer (1× PBS with 2% BSA (New England Biolabs, B9000S) and 0.02% Tween (Tween®-20 Solution ...
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bioRxiv - Cell Biology 2022Quote: Cytokinetic furrowing rate measurements for both the P0 cell and the AB cell were taken relative to nuclear envelope breakdown (NEB). For all analyses ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAP-cell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2-hour chase ...
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bioRxiv - Cell Biology 2022Quote: ... 3 h) the cells were incubated in 3 µM SNAP-Cell TMR-Star (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min and embedded using Vectashield (supplemented with DAPI).
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bioRxiv - Molecular Biology 2023Quote: ... The SNAP-tagged histones neosynthesized during the chase time were then pulse-labelled by incubating cells with 2 μM of the red-fluorescent SNAP reagent SNAP-cell TMR star (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (α-select, XL1-Blue, or BL21-DE3 cells were used, depending on application; all originally sourced from NEB and obtained from lab made stocks ...
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled with 100 nM Halo-TMR ligand and 100 nM SNAP-Cell 647-SiR ligand (New England Biolabs) for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells (α-select, XL1-Blue, or BL21-DE3 cells were used, depending on application; all originally sourced from NEB) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: Poly(A)+ RNA was isolated from nuclei and whole cells (see C2C12 cell fractionation above) using the Magnetic mRNA Isolation Kit (New England Biolabs). Two rounds of binding ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 30 min at 37°C in appropriate media containing 3µM of cell-permeable SNAP-Cell® 647-SiR (New England BioLabs). Cells were then rinsed twice with fresh media and incubated for 30min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Total SNAP-tagged histones were labeled by incubating cells with 2 µM of the red-fluorescent reagent SNAP-cell TMR-star (New England Biolabs) for 15 min before cell fixation ...
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bioRxiv - Developmental Biology 2023Quote: ... Isoseq library preparation was performed on 300 ng of RNA (RIN ≥ 9.3) of each cell type (isolated as described above) using NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification (NEB), Iso-Seq Express Oligo Kit (PacBio ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed directly in 8X the cell pellet volume with 2% SDS blue loading buffer (New England Biolabs), containing DTT ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were labelled both at the surface and intracellularly by addiction of the cell permeable SNAP-Cell 647 SiR ligand (1:1000, New England Biolabs) in DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Genomics 2021Quote: ... coli BL21 DE3 cells (C2527, New England Biolabs) using the DNEasy Blood and Tissue kit (69504 ...
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bioRxiv - Genomics 2020Quote: ... pTXB1 clones were transformed into BL21 cells (NEB) for production ...
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bioRxiv - Genomics 2020Quote: ... we transformed the reaction into electrocompetent cells (NEB), and obtained 1.44 million unique transformants ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21(DE3) cells (New England Biolabs C2527) with each plasmid and spread the transformed cells onto an agar plate (25 g/L LB ...
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bioRxiv - Bioengineering 2021Quote: ... coli cells (New England Biolabs, Cat No: C3029J).
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bioRxiv - Bioengineering 2020Quote: ... coli SHuffle® cells (New England Biolabs, USA) carrying our plasmids of interest were used for recombinant protein expression and were grown using Terrific Broth (TB ...
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bioRxiv - Biochemistry 2021Quote: ... T7 Express LysY competent cells from NEB (C3010I) were transformed with plasmid pEcoli-Cov_42 (Supplementary Table S2 ...
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bioRxiv - Neuroscience 2021Quote: ... DH5α NEB competent cells (New England Biolabs #C2987H) were transformed and Endofree Megaprep (Qiagen # 12381 ...
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bioRxiv - Microbiology 2021Quote: ... All plasmids were amplified in Turbo cells (NEB) and isolated using a high purity plasmid prep kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid was transformed into 10-beta cells (NEB) and clone sequence verified by sanger sequencing.
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB, catalog no C3040H, 30°C). To generate gp70 C.1086 V1V2 RQ(H/Y)173 constructs ...