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Citations for New England Biolabs :
1 - 50 of 930 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 22 μL of a mixture containing 10 mM of each of the four deoxynucleotide triphosphates (dNTPs) (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Plant Biology 2024Quote: Mutations (D to V) of the MHD motif were carried out using inverse PCR with primers containing AarI (NEB) or BsmBI (NEB ...
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bioRxiv - Systems Biology 2020Quote: ... 22 μL of 10 mM/each of four dNTPs (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 250 ng of DNA was digested with the restriction enzymes PstI (recognising the rare CTGCA/G motif) and MspI (recognising the frequent motif C/CGG; both enzymes from New England Biolabs, Ipswich, MA, USA). After the ligation of two adapters P1 and P2 ...
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bioRxiv - Genomics 2022Quote: ... and both motifs were deleted with Q5 SDM (NEB #E0554) using primers GWLP P20-23 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... Barcodes were amplified with 22 cycles of PCR using Phusion polymerase (NEB) and primers that add Illumina adapter sequences and a 6 bp identifier sequence used to distinguish cell populations ...
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bioRxiv - Biochemistry 2022Quote: ... This reaction was performed for 22 cycles using Q5 (New England Biolabs). These Illumina sequences were then used as the template for a second of PCR to add indexes and the p5 and p7 Illumina adapter sequence to make finished sequencing libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... Barcodes were amplified with 22 cycles of PCR using Phusion polymerase (NEB) with primers that add Illumina adaptor sequences and a 6 bp identifier sequence to distinguish the various drug conditions and time points ...
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bioRxiv - Bioengineering 2020Quote: ... The motif was inserted via NEBuilder® HiFi DNA Assembly (New England Biolabs): 35 ng of the digested vector ...
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bioRxiv - Genomics 2021Quote: ... at specific sequence motifs (GCTCTTCN^) in the presence of 1 μl 10X buffer 3.1 (NEB) for 2 h at 50 °C and ultra-pure water to a volume of 10 μl ...
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bioRxiv - Biochemistry 2019Quote: ... Chin (MRC-LMB, Cambridge, UK).[22] Enzymes for molecular cloning were purchased from NEB (Ipswich, MA) and used according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reactions were mixed to 22 µl including 10 U of HindIII-HF (NEB, Catalog #R3104L) and up to 2 µl of QuickExtract lysates ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 30 minutes in PBS containing the primary antibodies: rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs, Hangzhou, China), mouse monoclonal anti-tubulin β3 (801201 ...
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bioRxiv - Molecular Biology 2019Quote: ... ligation was performed during 4h at 22°C in the presence of truncated RNA ligase 2 (NEB)30 ...
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bioRxiv - Microbiology 2020Quote: ... RNA and 3′ adapters were incubated at 22°C for 2.5 hr with 51 U of T4 RNA Ligase I (NEB) and 12 U of recombinant RNase inhibitor (Takara Bio ...
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bioRxiv - Bioengineering 2021Quote: 2 μl of genomic DNA was used to amplify strain barcodes by PCR (Q5 NEB master mix, 22 cycles) using primers containing sequence-optimized spacers to maximize nucleotide diversity in Illumina sequencing ...
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bioRxiv - Genomics 2019Quote: ... and the mixture was incubated at 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H) for additional 5 minutes ...
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bioRxiv - Genomics 2022Quote: ... Purified genomic DNA was directly amplified by 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (NEB). Sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... RNA and 3′ adapters were incubated at 22°C for 2.5 hr with 51 U of T4 RNA Ligase I (NEB) and 12 U of recombinant RNase inhibitor (Takara Bio ...
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bioRxiv - Genomics 2023Quote: ... or 40,000 nuclei per well in 22 µL of NSB and 2 µL of 10 mM dNTP mix (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... a ~200 bp amplicon was generated from 1×106 template molecules by PCR in 22 cycles using Phusion polymerase (NEB), purified with the QIAquick gel extraction kit ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... The RNA fragments were ligated with 3’-barcoded adaptors at 22 °C for 1 h and 30 min using T4 RNA Ligase (NEB). Barcoded RNA samples were pooled together ...
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bioRxiv - Immunology 2022Quote: ... Ligation of the fragmented RNA to the MARS-seq adapter was performed at 22°C for 2h with T4 RNA ligase (NEB) followed by a 1.5X AMPure XP bead cleanup ...
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bioRxiv - Genomics 2023Quote: ... followed by the addition of 22 µL of ligation mix (20 µL quick ligase buffer and 2 µL of quick ligase, NEB) and incubation at 25°C for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... and sgRNA cassettes were amplified using 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (New England Biolabs). Sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
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bioRxiv - Neuroscience 2024Quote: ... The UNC13A CE was amplified with a forward primer in exon 19 5’-CAGACGATCATTGAGGTGCG-3’and reverse primer in exon 22 5’-ATACTTGGAGGAGAGGCAGG-3’using Q5 High Fidelity Master Mix (NEB). PCR products were resolved on a TapeStation 4200 (Agilent ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations into the Snail binding motif of the Crbn regulatory sequences were obtained by recombination using NEBuilder (NEB England) of PCR amplified fragments of the Crbn reporter constructs (primers in Supp Table 6) ...
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bioRxiv - Molecular Biology 2019Quote: ... A second adaptor was added to the cDNA by adding 50 pmol 5iLL-22 DNA adaptor (Table S6) and ligating with T4 RNA Ligase 1 (NEB, M0437M) at 23°C for 3 hrs ...
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bioRxiv - Genomics 2019Quote: ... the temperature was slowly ramped down (−0.1°C/sec) to 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H), and the mixture was incubated at 45°C (for Hybridase RNase H ...
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bioRxiv - Microbiology 2024Quote: nfsA and nfsB mutants were constructed by gene inactivation using the pKNOCK suicide plasmid (22) The DNA fragments were amplified with Phusion high-fidelity DNA polymerase (NEB, UK) from E ...
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bioRxiv - Bioengineering 2023Quote: ... then the mixture was cooled to room temperature (∼22 °C) prior to immediate transformation into NEB Stable chemically competent bacteria (NEB #C3040H).
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bioRxiv - Molecular Biology 2021Quote: ... the pyrimidines present in the TOP motif of the reporters were replaced with purines using Q5 site directed mutagenesis kit (NEB) as per the manufacturer’s protocol using the oligos listed in the Supplementary file 1 ...
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bioRxiv - Plant Biology 2022Quote: ... The potential gRNAs were also screened to identify those with restriction enzyme recognition motifs (from a list commercially available enzymes from NEB) that overlapped position 17-18 of the gRNA (between position 3 and 4 bp from the PAM ...
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bioRxiv - Biochemistry 2024Quote: ... gRNA targeting a 20 nt genomic region adjacent to a Cas9 protospacer adjacent motif (PAM) was first annealed and cloned into BsmbI (NEB) digested pLentiCRISPRv2 vector ...
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bioRxiv - Microbiology 2023Quote: ... containing ligase T4 (NEB), and its reaction buffer ...
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bioRxiv - Microbiology 2019Quote: ... The Gag-PYQE-mCherry plasmid was modified by site-directed mutagenesis to make a base pair substitution mutation (C to A) that changes the PYQE motif to PYKE using the Q5 SDM kit (New England Biolabs, USA). Gag-PYQE-mCherry plasmid was linearized by PCR using 5’-AAGGAGCCTCTGACGAGCC-3’ as forward primer (with the C to A base pair substitution underlined ...
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bioRxiv - Cell Biology 2022Quote: ... and the region surrounding the protospacer adjacent motif (PAM) was amplified with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, #M0530). PCR products were purified using the QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Biochemistry 2020Quote: ... or a control motif (5’-GGGACCCTGGGAGGG-3’) were prepared by viral replication using a helper phage M13K07 (New England BioLabs, Cat#N0315S). E.coli XL1-Blue cells were transformed with pBluescript SK(- ...
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bioRxiv - Microbiology 2019Quote: ... coli ER1821 containing pM.EsaBC4I (NEB) (50 ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis of ATG8-Interacting Motifs (AIM) was performed using Q5 site-directed mutagenesis kit (New England BioLabs, Frankfurt am Main, Germany) using primers listed in Supplemental Table S5 according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... containing DNase I (New England Biolabs) and 1× cOmplete ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.2 mg/mL BSA (NEB), 180 μM dNTPs and 10 units of phi29 DNA polymerase (NEB ...
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bioRxiv - Genomics 2020Quote: ... containing 1.25U E.coli PolyA (NEB M0276S), 1.25X PolyA buffer (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... containing 1x rCutSmart buffer (NEB, B6004S), and 5U of quick CIP (NEB ...
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bioRxiv - Microbiology 2023Quote: ... containing 1x NEBuffer 2 (NEB, B7002S), 1 mM ATP ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mM Spermidine) containing MNase (NEB, M0247S) to final concentration of final concentration of 20 U/µL and incubated at 37°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... containing 5 U murine RNase Inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
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bioRxiv - Immunology 2020Quote: ... containing 200 U PNGaseF (New England Biolabs) or buffer only ...