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Citations for New England Biolabs :
451 - 500 of 877 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sense and antisense oligos containing the 20 bp guide target sequence wereannealed and phosphorylated with T4 Polynucleotide Kinase (NEB), then inserted between BbsI sites in the plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... were generated using the vPRC1.4 recombined plasmid (containing all three expression cassettes) as template and using Phusion polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing CrtSPARTA protein complex was pooled and further loaded onto an amylose resin column (New England Biolabs). The column was washed with 20 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: A 10 µL reaction containing 200 ng of pTrc200HA plasmid DNA and 1× CutSmart Buffer (New England BioLabs, USA) with partially purified V2c or its variants normalized by the major V2c protein band was incubated at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... The next day samples were washed twice with dig-wash (wash buffer with 0.025% digitonin) and then resuspended in 150 µl dig-wash containing pA-MNase (New England Biolabs 700 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: Mutant variants of the mS29-containing construct were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). Mutagenesis primers were designed using the NEBaseChanger tool ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... a mix containing 18µM sgRNA and 12µM Cas9 protein (EnGen™ Spy Cas9-NLS S. pyogenes, NEB Cat# M0646T) was incubated for 10 min at room temperature ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...
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bioRxiv - Genetics 2024Quote: ... in reactions containing 1X ThermoPol buffer (12.5mM tricine, 42.5mM KCl, 1.5mM MgCl2, 6% dextran; New England BioLabs, Ipswich, MA), 0.32 mM dNTP mix ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the annealed oligos were phosphorylated in a 10 µl reaction containing 0.25 µl T4 PNK (10000 units/ml, NEB) and 1 µl 10x T4 ligase buffer for 40 min at 37°Ϲ before heat-inactivation at 65°Ϲ for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... and detected using anti-MBP antibodies (New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse antibodies against MBP (NEB E8032L, 1:5000) and RAD51 (Novus Biologicals 14b4 ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated overnight with anti-SNAP antibody (NEB, Ipswich, MA), washed and probed with goat-anti-rabbit horseradish peroxidase secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... and detected using the anti-MBP-HRP antibody (NEB). The bound protein (~42kDa ...
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bioRxiv - Neuroscience 2022Quote: ... and probed with p62 antibody (NEB D5L7G, 1:800) and streptavidin-594 (Biolegend 405240 ...
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bioRxiv - Molecular Biology 2022Quote: Anti-M13-pIII monoclonal antibody (E8033S, New England Biolabs), sheep anti-mouse IgG (H/L):HRP (AAC10P ...
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bioRxiv - Neuroscience 2023Quote: ... TCF/LEF family antibody sampler kit (New England BioLabs), NFAT1 (New England BioLabs) ...
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bioRxiv - Plant Biology 2023Quote: ... after which an anti-p42/p44-erk antibody (NEB) was employed to detect the activated MAPKs on western blots.
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bioRxiv - Cell Biology 2023Quote: ... anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-clathrin heavy chain antibody (BD Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... and detected using anti-MBP antibodies (New England Biolabs). As a negative control ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA resin elute was immediately diluted with PBS containing 1 mM EDTA (PBS-E) and incubated with amylose resin (New England Biolabs) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was then diluted 1:10 with nuclease free water prior to adding 1 µL to a PCR reaction containing 500 uM forward and reverse primer and 1X Q5 Master Mix (NEB). Reactions were cycled 35 times with annealing temperatures calculated by NEB Tm calculator prior to loading on 2% agarose gels ...
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bioRxiv - Genetics 2021Quote: ... derived from pEAA238-PMS1) containing FRB or FKBP insertions were constructed using HiFi Gibson cloning (New England Biolabs, Ipswich, MA), with PCR fragments generated from pEAA213 or pEAA238 ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 μM of complementary primers containing 20 bp overlap - target sequence - and 4 bp of homology to the expression vector were hybridized in a reaction containing 10 μL of 10x T4-ligase buffer (New England Biolabs). The mixture was submitted to 70 decreasing cycles of a minute each - touchdown - from 95 to 25°C and then connected to the previously linearized vector through a reaction containing 15 ng of the previous hybridization ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Supplementary Data 1) P1 adapters (200 nM) containing unique 12 bp barcodes were ligated by incubation with T4 ligase (NEB) at room temperature for 30 min and the reaction was terminated by 20 min at 65°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... this region was amplified using 10 separate pools of 33 nt primers containing NNN in the middle of the primer and using Gibson Assembly Master Mix (NEB) was cloned into pNL4-3 rev-in-nef after digesting by BamHI and XhoI ...
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bioRxiv - Genomics 2020Quote: ... Beads containing the ssDNA extension products were suspended in 10 μl of polyadenylation master mix containing 2 units of terminal transferase TdT (New England Biolabs), 1x TdT buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... then overnight at 25°C with another 100 μl of the same buffer containing 2.7 μl END-seq adaptor 1 and 1 μl high concentration T4 DNA Ligase (NEB M0202M). After rinsing twice with 1 ml tris buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... were separately subjected to restriction enzyme double digestion at 37 °C overnight in 20 μL volumes containing 20 U BamHI-HF (cat. no. R3136S, New England BioLabs), 20 U EcoRI-HF (cat ...
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bioRxiv - Molecular Biology 2019Quote: ... a DNA fragment containing TDP-43 was inserted into pCold I-GstTev-HA by NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... In-house adapters containing 8-nucleotide unique molecular identifier (UMI) sequences were ligated onto the DNA fragments with T4 DNA ligase (NEB). Excess adapters were removed by AMPure XP beads (Agencourt ...
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bioRxiv - Genomics 2019Quote: ... We quantified DNA using a Qubit dsDNA BR assay and performed a debranching treatment on 8-10 µg of DNA in 100 µL reactions containing 50 Units of endonuclease T7E1 (New England Biolabs) in 1X NEB buffer 2 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction mixture was then dried completely and resuspended in a mixture containing 50 mM ammonium bicarbonate and PNGase F (New England Biolabs) using only H2O18 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2019Quote: ... By mixing the linearized pCRISPR-BEST plasmid and chemically synthesized spacer containing oligo with the NEBuilder (New England Biolabs, USA). The linearized pCRISPR-BEST plasmid then will be bridged by the spacer containing oligo ...
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bioRxiv - Biochemistry 2020Quote: Site-directed mutagenesis was carried out by PCR amplification of the starting plasmid with forward and reverse mutagenesis primers containing the desired mutation (Table 1) followed by DpnI (NEB) treatment ...
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bioRxiv - Developmental Biology 2019Quote: ... Repair templates were generated by inserting 500 bp homology arm PCR products into destination vectors containing egfp and a self-excising selection cassette using Gibson assembly (New England Biolabs) or SapTrap assembly (Dickinson et al ...
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bioRxiv - Molecular Biology 2019Quote: ... Both 3' and 5' ligations were carried out with degenerate adaptors (each containing 4 random nucleotides) in the presence of 10% Polyethylene Glycol (PEG 8000, NEB) and 0.5 μL of Superasin (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 1 µL was used as the template in a PCR amplification containing 0.5 µL of Q5 High-Fidelity DNA Polymerase (NEB, M0491S), 1x Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Genomics 2020Quote: Cas9 protein and transcribed sgRNA were incubated for 10 min at room temperature in reaction buffer containing 1× NEB buffer 3.1 (NEB Biolabs) supplemented with 1 mM DTT ...
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bioRxiv - Genomics 2019Quote: ... we eluted the RNA by adding 4 µl of master mix containing 1 µl of 10 mM dNTPs (New England Biolabs), 0.1 µl of 100 µM 3’ SMART reverse transcriptase (RT ...
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bioRxiv - Microbiology 2019Quote: ... Amplification was performed in a total volume of 25 µl containing 12.5 µl of 2x OneTaq Mastermix (New England Biolabs, NEB), 9.9 µl of nuclease-free H2O ...
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bioRxiv - Microbiology 2019Quote: ... Alexa fluor phalloidin 647 was used to stain the actin cytoskeleton and cell nuclei were stained with ProLong Gold Antifade mountant containing 4,6-diamidino-2-phenylindole (DAPI) (New England Biolabs, UK). To determine mucus production ...
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bioRxiv - Microbiology 2019Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
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bioRxiv - Physiology 2020Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Plant Biology 2019Quote: ... RT-PCR reaction volumes were set up to 12.5 μL containing Luna Universal qPCR Master Mix (New England Biolabs Inc.), 2 μl of a 1:10 dilution of cDNA reaction ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
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bioRxiv - Genomics 2021Quote: ... Methylation reactions were performed in a 100uL reaction containing 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated at 37°C for 30 minutes ...