Labshake search
Citations for New England Biolabs :
651 - 700 of 1339 citations for Synapsin I derived peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... Template were first digested with T7 endonuclease I (M0302L, NEB, USA) as suggested in the protocol available on the ONT community website ...
-
bioRxiv - Microbiology 2023Quote: ... sterile water and then treated with DNase I (New England Biolabs) for 60 mins at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... Purified PCR products were then treated with Exonuclease I (NEB M0568L) and purified using 1× AMPure XP beads (Beckman Coulter A63881) ...
-
bioRxiv - Biochemistry 2023Quote: ... incubated with Dpn I (New England Biolabs Inc., Ipswich, MA, USA) and transformed into E ...
-
bioRxiv - Genomics 2023Quote: ... Purified PCR products were then treated with Exonuclease I (NEB, M0568L) and purified using 1× AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Biochemistry 2023Quote: ... or double-stranded DNA (New England Biolabs, M13mp18 RF I DNA).
-
bioRxiv - Cancer Biology 2023Quote: ... and 1 µL of each Exonuclease I (NEB, cat. no. M0293S) and Exonuclease III (NEB ...
-
bioRxiv - Cell Biology 2023Quote: ... blunted using DNA polymerase I large Klenow fragment (New England Biolabs) and inserted into a PmeI-linearized pWPI Puro lentiviral vector carrying a puromycin resistance gene.
-
bioRxiv - Microbiology 2023Quote: ... and DNase treatment using DNase I (New England Biolabs, Ipswich, USA). Library preparation and sequencing were performed by the Australian Genome Research Facility (https://www.agrf.org.au/) ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 μL of 10x DNase I Buffer (New England Biolabs, #m0303s), 1 μL of rDNase I (ThermoFisher Scientific ...
-
bioRxiv - Genetics 2023Quote: ... the mixture was treated with 1ul Exonuclease I (NEB, Ipswich, MA) to remove excess ssDNA ...
-
bioRxiv - Immunology 2023Quote: ... and 10 μg/mL DNase I (New England Biolabs, Cat#M0303S) for continuous passages in HFFs ...
-
bioRxiv - Genomics 2023Quote: ... dTTP and biotin-dATP using Klenow DNA polymerase I (NEB M0212L), followed by in situ proximity ligation at room temperature overnight ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... dGTP and dTTP by DNA Polymerase I Klenow Fragment (NEB, #M0210L). The resulting blunt-end fragments were religated by T4 DNA ligase (Enzymatics ...
-
bioRxiv - Bioengineering 2023Quote: ... RNA extraction was performed and RNA treated with DNase I (NEB). The quality of the extraction was verified by agarose gel electrophoresis ...
-
bioRxiv - Genomics 2023Quote: ... and 50U of Klenow fragment of DNA I polymerase (NEB, M0210L) were added to the remaining material in order to repair DNA ends ...
-
bioRxiv - Immunology 2024Quote: ... 1 µL of 2000 U/mL DNase I (New England Biolabs) was added to bring the final reaction volume to 10 µL ...
-
bioRxiv - Systems Biology 2024Quote: ... Samples were then treated with 4 units of DNase I (NEB) for 1 hr at 37°C ...
-
bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Exonuclease digestion was then performed with thermolabile exonuclease I (NEB, M05685) to remove excess primers (37°C for 4 minutes then 80°C for 1 minute) ...
-
bioRxiv - Physiology 2024Quote: ... The flow cell was then treated with exonuclease I (M2903, NEB) cocktail in 37 °C for 45 min to remove non-specific single-stranded DNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... and NEB MultiplexOligo sets I/II/III/IV (NewEngland Biolabs, E7500L) without size selection according to the manufacturer’s protocol with modifications ...
-
bioRxiv - Molecular Biology 2024Quote: ... the DNA template was removed by treatment with DNase I (NEB), followed by purification of the RNA transcripts using the RNeasy Kit (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... 20 mM MgCl2 and digested with 10 μg DNAse I (NEB) and 50 μg RNAse (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then incubated with 2ul of DNase I (New England Biolabs), 2ul RNase A (Invitrogen) ...
-
bioRxiv - Biochemistry 2024Quote: ... the reactions were treated twice with DNAse I (New England Biolabs) and quenched with 10% volume of 0.5 M EDTA pH 8.0 ...
-
bioRxiv - Developmental Biology 2020Quote: ... RFP-histone H2B fusion protein (pRN3-C-term-RFP-Hist1h2bb) derived by in frame cloning of a high-fidelity PCR amplified cDNA (Phusion® DNA polymerase, New England BioLabs, M05305) encoding histone H2B (Hist1h2bb ...
-
bioRxiv - Biochemistry 2020Quote: ... The DNA fragment encoding Sd223-440 was PCR amplified and inserted into a pET28-derived vector providing an N-terminal His-HRV3C-Avi-tag by HiFi DNA Assembly Cloning (New England Biolabs, Ipswich, MA) according to the instructions of the manufacturer ...
-
bioRxiv - Biochemistry 2020Quote: ... was PCR amplified and inserted into a pET28-derived vector providing an N-terminal His-HRV3C-tag by HiFi DNA Assembly Cloning (New England Biolabs, Ipswich, MA) according to the instructions of the manufacturer ...
-
bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...
-
bioRxiv - Genomics 2019Quote: ... The available CPQ cDNA was previously prepared from total RNA derived from midguts of fifth-instar larvae fed on semi-synthetic diet using a Protoscript II kit (NEB, Ipswich, MA). PCR was carried out on both strains using primers designed to flank the region of the kdr mutation in the para gene (Forward 5’-ACCAAGGTGGAACTTCACAGAT −3’ Reverse 5’-AGCAATTTCAAGAAGTCAGCAACA −3’) ...
-
NSC-derived exosomes enhance therapeutic effects of NSC transplantation on cerebral ischemia in micebioRxiv - Neuroscience 2022Quote: Sequencing libraries of miRNAs of NSC-derived exosomes were produced using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, United States) following the previous report (Cai et al. ...
-
bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
-
bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
-
bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... g165 and g166 without the predicted signal peptides were PCR-amplified with Phusion polymerase (NEB) from genomic DNA of P ...
-
bioRxiv - Molecular Biology 2023Quote: ... to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB, R0174S) to allow for exoIII digestion towards the candidate protein ...
-
bioRxiv - Biochemistry 2023Quote: ... Desalted peptides were dissolved in 1 ml 1 × CutSmart buffer (pH 8, New England BioLabs) and incubated with 50 units of alkaline phosphatase (calf intestinal ...
-
bioRxiv - Genetics 2021Quote: ... and the sample was digested with DNase I (NEB [1:20 dilution]) for 30 seconds at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... which required digestion with Sma I (New England Biolabs® Inc., U.S.A).
-
bioRxiv - Evolutionary Biology 2021Quote: ... and 3 μL DNase I (both New England Biolabs, Ipswich, MA, USA) was added to 30 μL of the mixture and incubated for 22 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... Heterozygosity was assessed with T7 endonuclease I cleavage (New England Biolabs, #M0302S) upon direct incubation of 10 μl amplicon ...
-
bioRxiv - Biophysics 2019Quote: ... linearized with Not I enzyme (New England Biolabs, Inc., Ipswich, MA, USA) and transcribed in vitro using the T7 promoter mMESSAGE cRNA kit (Ambion ...
-
bioRxiv - Developmental Biology 2019Quote: ... Each sample was treated with DNAse I (New England Biolabs, Ipswich, MA) to remove any residual genomic DNA contamination ...
-
bioRxiv - Pathology 2019Quote: ... 1 μL (2 units) DNAse I (M0303S, New England BioLabs, Ipswich, MA), and 89 μL nuclease-free H2O ...
-
bioRxiv - Genetics 2019Quote: ... and 1µl of 5 U/μl large DNA Polymerase I (NEB, M0210L) were added ...
-
bioRxiv - Genomics 2019Quote: ... and pcDNA3.1-Mb3Cas12a-mSA were digested with EcoR I (Cat. R3101S, NEB) overnight at 37°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... The PCR products were digested with T7 Endonuclease I (T7E1, NEB M0302S) according to the recommended protocol36 ...
-
bioRxiv - Cell Biology 2021Quote: ... instead of dCTP and 5 U/μl Klenow fragment Dpol I (NEB) at 37°C for 2 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 μl of T4 RNA ligase I (10U final, NEB M0437M) were combined in 20 μl total volume and incubated for 16 h at 16 °C ...