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Citations for New England Biolabs :
451 - 500 of 1339 citations for Synapsin I derived peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... using DNA Polymerase I and Large (Klenow) Fragment (NEB). Blunt-ends were ligated with 4000 units T4 DNA Ligase and chromatin de-crosslinked using de-crosslinking buffer (50 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
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bioRxiv - Physiology 2024Quote: Tissue sections were treated with exonuclease I (#M2903, NEB) cocktail the next day in 37 °C for 45 min to remove unused single-stranded DNA probes ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently treated with DNase I (New England Biolabs). 400-500 ±g of protein was then added to 50 µl of Wheat Germ Agglutinin (WGA ...
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bioRxiv - Microbiology 2024Quote: ... treated with DNase I (NEB, cat# 50-814-118) and cDNA was prepared with the High Capacity cDNA reverse transcriptase kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess RT primers were digested using Exo I (NEB). RNA was hydrolyzed by NaOH (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.3 μl of T4 DNA ligase I (NEB, M0202L), 6.25-µl of double-stranded barcoded oligonucleotides (1st set of sub-barcodes ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the T7 Endonuclease I (T7EI) assay (NEB).
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bioRxiv - Synthetic Biology 2023Quote: ... then directly treated with DNAse I-XT (NEB M0570) at 37°C for 30 minutes to remove DNA template ...
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bioRxiv - Microbiology 2023Quote: ... putative RNA samples were incubated with DNase I (NEB) for 1 hour at 37 ºC and inactivated afterwards with EDTA at a final concentration of 5 mM ...
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bioRxiv - Biochemistry 2021Quote: Peptide-N-glycosidase F (PNGase F) (New England Biolabs Inc., Cat. P0704S) was used for complete removal of N-linked oligosaccharides ...
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bioRxiv - Plant Biology 2020Quote: ... 1.5 μg of biotinylated histone peptides were incubated with streptavidin beads (NEB) in binding buffer (50 mM Tris-HCl 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... separated via self-cleaving T2A peptide was generated via Gibson Assembly (NEB). This cassette including a 5’ chimeric intron and 3’ poly adenylation signal sequence replaces the sequence of Pax7 exon 1 immediately 3’ of the ATG start codon ...
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bioRxiv - Genomics 2021Quote: ... specified amounts of MCF7 genomic DNA or plasma-derived cfDNA were digested with LpnPI (New England Biolabs, Ipswich, MA) yielding 32 bp fragments around the fully methylated recognition site containing a CpG ...
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bioRxiv - Molecular Biology 2021Quote: ... Two separate 8-µl aliquots of beads derived from each strain were treated with 1 µl lambda phosphatase (NEB) in 10 µl reactions containing ...
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bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Kinase-dead mutant (K37R) of Nek2A was derived from Nek2A in pJP1563 using Q5 Site-Directed Mutagenesis Kit (NEB) following the instructions of manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2) were digested with PvuII (New England Biolabs) and subsequently gel isolated ...
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bioRxiv - Microbiology 2024Quote: PCR products of Asaia positive samples were cleaned up using Exonuclease I (Exo I) and Shrimp Alkaline Phosphate (Exo-SAP protocol) according to the protocol of New England Biolabs (NEB, MA, and USA) and sent for sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... DNase I treatment was performed to denaturalize the genomic DNA in the cells using 20U DNase I for 40s per coverslip (NEB; DNase I (RNase-free), M0303S) ...
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bioRxiv - Genetics 2021Quote: ... derived from pEAA238-PMS1) containing FRB or FKBP insertions were constructed using HiFi Gibson cloning (New England Biolabs, Ipswich, MA), with PCR fragments generated from pEAA213 or pEAA238 ...
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bioRxiv - Microbiology 2021Quote: The chimeric CrPV-aNCV infectious clone was derived from the full-length CrPV infectious clone (pCrPV-3; Accession KP974707) (35) using Gibson assembly (New England BioLabs), per the manufacturer's instructions ...
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bioRxiv - Molecular Biology 2022Quote: The coding sequence of the BTB domain of selected ZBTB family proteins was amplified from cDNA derived from the human HCT116 cell line using Q5 High-Fidelity DNA Polymerase (NEB). Specifically for the Patz1 construct ...
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bioRxiv - Cell Biology 2021Quote: ... were expressed with an N-terminal His8 tag followed by a 3C protease site from a pBAD-TOPO derived plasmid in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
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bioRxiv - Cell Biology 2019Quote: ... were derived from pSS393 by using the Q5 site-directed mutagenesis procedure (pSS396) or the NEBuilder assembly kit (New England Biolabs) (pSS397) ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA (5-10 μg) derived from transfected ESC was digested by high concentrated HindIII-HF or high concentrated BamHI-HF (NEB). In all Southern blot analyses ...
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bioRxiv - Molecular Biology 2022Quote: ... Other G2L4 and GII RT mutant plasmids were derived from pMal-G2L4 RT or pMal-GII RT by using a Q5 mutagenesis kit (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: ... pGL-CPERlinker was assembled from synthetic DNA oligonucleotides and fragments (IDT) as well as the ampicillin resistance cassette and the origin of replication derived from pUC19 (NEB).
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bioRxiv - Cell Biology 2023Quote: ... The pSS459 plasmid driving the expression of PHOP1-GFP-pch2-nes4A was derived from pSS393 by using the NEBuilder assembly kit (New England Biolabs) and a synthesized gBlock fragment (IDT ...
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bioRxiv - Synthetic Biology 2024Quote: ... See Table S1 for a full list of derived yeast strains. NEB Turbo competent Escherichia coli (E. coli) from New England Biolabs (NEB was used for all DNA cloning and plasmid propagation work.
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bioRxiv - Cancer Biology 2021Quote: ... 5U of T7 Endonuclease I (New England BioLabs® M0302L) was added and the reaction was incubated at 37°C for 20 minutes ...
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bioRxiv - Genetics 2021Quote: ... Digestions were performed with NciI I et BsaXI (NEB, USA) for TYR - alleles c and Ch ...
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bioRxiv - Developmental Biology 2020Quote: ... and treated with exonuclease I (New England BioLabs, NEB #M0293S). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method ...
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bioRxiv - Developmental Biology 2020Quote: ... and treated with exonuclease I (New England BioLabs, NEB #M0293S). Double-stranded cDNA was generated by the second strand synthesis via the nick translation method ...
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bioRxiv - Molecular Biology 2022Quote: The RNA was then treated with DNase I (NEB M0303S) to remove trace amount of genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Excess RT primers were removed with exonuclease I (M0293S, NEB), and the cDNAs were size-selected (120-220 nt ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA probes were removed by 2.5 μL DNase I (NEB) with 3 μL 10× DNase I buffer at a final volume of 30 μL ...
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bioRxiv - Immunology 2019Quote: ... Preamplified cDNAs were treated with Exonuclease I (New England Biolabs) to remove unincorporated primers and the preamplified cDNAs and detection assays were loaded onto a 96.96 Dynamic Array IFC (Fluidigm) ...
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bioRxiv - Genomics 2019Quote: ... the samples were incubated with 1µL Exonuclease I (NEB, #M0293) and 2 µL of 10X reaction buffer at 37°C for 30 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... unused primers are degraded with Exonuclease I (New England Biolabs), beads are washed and PCR amplified ...
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bioRxiv - Genomics 2019Quote: ... tissue sections were gently permeabilized using exonuclease I buffer (NEB) and pepsin ...
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bioRxiv - Genomics 2020Quote: ... The resulting total RNA was treated with DNase I (NEB) to avoid genomic DNA contamination ...
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bioRxiv - Genetics 2021Quote: ... The eluted RNA was then treated with DNAse I (NEB) and then cleaned and concentrated using a classical phenol-chloroform RNA extraction ...
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bioRxiv - Genetics 2020Quote: ... or dsDNA (Phi 174 RF I, NEB; linearized with ApaLI) in E buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... and digested overnight with <ι>βi>-agarase (NEB M0392). DNA combing was performed using commercially available coverslips (Genomic Vision COV-001) ...
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bioRxiv - Genomics 2021Quote: ... and 4 μl 5 U/μL I-SceI (NEB, #R0694L) in a 50 μL final volume for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... These constructs were linearised with Not I (NEB, Ipswich, USA) and then mRNA was transcribed in vitro using the mMESSAGE mMACHINE T7 in vitro transcription kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Cell Biology 2021Quote: ... the beads were treated with Exonuclease I (New England Biolabs) to obtain single-cell transcriptomes attached to microparticles (STAMP) ...