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Citations for New England Biolabs :
551 - 600 of 1699 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 50 mM Tris-Cl pH 7.4, 12 mM MgCl2, 1% NP-40, 0.5 mM DTT, 0.1 mg/ml cycloheximide, 80 U/ml NEB murine RNase inhibitor). Beads were transferred to a fresh tube and immunoprecipitated RNA extracted by incubating beads in buffer RLT (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: aPSM and pPSM specimens were separately diced and incubated with proteinase K (10μL at 20mg/mL U/mL, New England Biolabs, Ipswich, MA) at 37 °C - overnight to digest protein components ...
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bioRxiv - Biophysics 2022Quote: ... A low-pressure chromatography column connected to a peristaltic pump (5 mL/min flow rate) was prepared with 5 mL amylose resin (NEB #E8021S), followed by equilibration with 50 mL Amylose A Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The plugs were washed in 20 ml of Milli-Q water for 30 minutes and then 10 ml of 1x rCutSmart buffer (New England Biolabs, B6004S) for 1 hour followed by a further hour in 5 ml of 1x rCutSmart buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 100 ng of DNA were digested using PstI and MspI (New England Biolabs, Ipswich, MA) after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: 5-7 μg of HiC library in a total volume of 100 μl (1x NEB buffer 2.1 ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB) by repeating resuspending/pelleting.
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bioRxiv - Biochemistry 2020Quote: 100 ng of purified tsRNAs were treated with 0.5 U/µL T4 polynucleotide kinase (NEB) and 166 µM ATP (NEB ...
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bioRxiv - Microbiology 2019Quote: ... was obtained by adding 100 units of calf-intestinal alkaline phosphatase (CIP) (New England Biolabs) and 2mg/ml RNase A (Sigma) ...
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bioRxiv - Genetics 2019Quote: ... Using the 100 bp and 1 kb DNA Quick-Load ladders (N0467 and N0468, NEB) as a reference ...
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bioRxiv - Plant Biology 2020Quote: ... 100 ng of DNA was digested using PstI and BfaI (New England Biolabs, Ipswich, MA) after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was then resuspended in 100 μl/sample volume of 1X NEBuffer 2 (NEB) supplemented with Triton X-100 to 0.1% and transferred to a new tube ...
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bioRxiv - Biochemistry 2022Quote: ... 29 μl of purified modified RNA was added to 100 ng Random Primer 9 (NEB) and 0.2 mM of each dNTP ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in a 100 μL blunt enzyme mix reaction (Quick Blunting Kit, NEB) with half the enzyme concentration of the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... A half of DNA was digested with 100 units of XhoI (New England Biolabs (NEB), Ipswic ...
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bioRxiv - Microbiology 2020Quote: ... A half of DNA was digested with 100 units of XhoI (New England Biolabs (NEB), Ipswic ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng of DNA was treated with or without 6.6 units of ExoV (RecBCD, NEB) in a 10 µl reaction volume and incubated for 1 h at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomes were added to 100 nM to the PURExpress ribosome-free kit (New England Biolabs) (29) ...
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bioRxiv - Molecular Biology 2020Quote: ... and treated amplicons were visualized on ~1% agarose gels with 100 bp DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μl of a master mix that contained 1X DNA Adenylation Buffer (New England Biolabs), 100 μM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... Diluted cDNA (100-fold) was used for two multiplex PCR reactions with Q5 polymerase (NEB) with two primer pools (Table S5) ...
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bioRxiv - Plant Biology 2023Quote: ... For size comparison a radioactively labelled DNA ladder (100 bp DNA ladder, New England Biolabs) was applied.
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bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
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bioRxiv - Cancer Biology 2023Quote: ... Crosslinked nuclei were first resuspended in 100 µl 1x r2.1 restriction buffer (New England BioLabs) and then treated with 2% SDS for 1h at 37°C and with 1% Triton X-100 for 1h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... treating each well with 100 μl of lysis buffer (0.45% NP40, 0.45% Tween20, 0.5x NEB Q5 PCR buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
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bioRxiv - Plant Biology 2023Quote: ... 100 µg of dual crosslinked enriched chloroplasts were resuspended in 1X DpnII reaction buffer (NEB) and digested with 20 U DpnII at 37°C overnight with gentle shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 25°C in 100 μl 1x T4 RNA ligase buffer (NEB) containing 40 μl 50% PEG 8000 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Biophysics 2023Quote: ... 100 μg of each RBD was treated with endo F3 (purchased from New England Biolabs) in 1x Glycobuffer (50 mM sodium acetate ...
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bioRxiv - Neuroscience 2024Quote: ... An imaging activation mix was prepared by adding RNAse inhibitor (100 μL, New England BioLabs) to 250 μL Imaging Buffer Activator (VIZGEN ...
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bioRxiv - Genetics 2021Quote: ... and 0.1 mg/ml Proteinase K (New England Biolabs). DNA was electrophoresed in 1.2% agarose gels in 1xTAE (Tris-acetate-EDTA ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was incubated with 0.75 mL amylose resin (NEB) for 2 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.1 mg/ml Proteinase K (New England Biolabs) and incubated at 37 °C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 700µl G2 and 50µl Proteinase K (800U/ml, NEB) was added to each tube and incubated at 50°C for 2h with occasional tube inversions ...
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bioRxiv - Genomics 2020Quote: ... One microlitre of T4 DNA ligase (NEB, 400,000U/mL) was added to the reaction and incubated at 16 °C to ligate inserts ...
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bioRxiv - Genomics 2020Quote: ... 0.25 μl SbfI-HiFi (NEB 20,000 U/ml R3642L) and 4 μl of 10X cutsmart buffer ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl T4 Ligase (NEB, 400,000 U/ml m0202L), 6 μl of 10X T4 ligase buffer and 11.5μl water were added directly to the digested DNA ...
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bioRxiv - Genomics 2020Quote: ... using 0.25 μl MspI (NEB, 20,000 U/ml R0106S), 0.25 μl SbfI-HiFi (NEB 20,000 U/ml R3642L ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL 1 mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 400 U/µL (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... 8 μL of BSA (20 mg/mL B9000S, NEB,) and 100 Units of ligase (M0202M ...
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bioRxiv - Microbiology 2020Quote: ... A DNase I RNAse free 2000 U/ml (NEB) treatment was done using 1 μl for 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 5 ml 1x NEB Buffer 2 (NEB) and drop frozen in liquid nitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... 0.04 units/mL DNase I (New England Biolabs, Inc.) pH 7.5-8.0) ...
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bioRxiv - Molecular Biology 2020Quote: 5 μl Bst 3.0 (NEB, M0374L, 8,000 units/ml),
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bioRxiv - Biophysics 2021Quote: ... 0.2 unit/mL yeast inorganic pyrophosphatase (New England Biolabs) [28] ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL of 5000 units/mL RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of Endonuclease IV (10,000 U/ml, NEB) was added to the reaction ...