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Citations for New England Biolabs :
451 - 500 of 1699 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 100 ng/µL injection mix (70 ng/µL 1 kb DNA ladder (NEB), 30 ng/µL plasmid of interest ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... proteins (2 mg mL-1) were incubated with 180000 U mL-1 PNGase F (New England Biolabs, Unites States) in PBS (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... 16.4 mL elution was taken into 40 mL ligation reaction (13 Quick ligase buffer [NEB], 4000U Quick ligase [NEB] ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant was removed using a magnetic separator and washed once with 1 mL NEBuffer 2 and resuspended in 115 mL of Ligation reaction with Quick Ligase buffer (NEB), 6,000 U of Quick Ligase (NEB ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions (2×1012 particles/ml) were incubated in the presence of 10 μg/ml endoproteinase LysC (New England Biolabs) at 37°C in a S1000 thermal cycler (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... Washed resins were resuspended in 1 CV of buffer A and mixed with 10xHis-tagged Gi heterotrimer at the ratio of 2 mg Gi per ml resin with the presence of 25 mU/ml apyrase (New England Biolabs). The resuspended resin/Gi mixture was subjected to irradiation with 495-nm long-passed filter for 10-15 minutes to induce activation of rhodopsin and G protein binding ...
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bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Genomics 2023Quote: ... and washed twice with 2 mL of CLB containing Superase-In and 1% v/v of 20 mg/mL molecular grade BSA (NEB). After the final wash ...
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bioRxiv - Neuroscience 2023Quote: ... and the samples were resuspended in cold Nuclei suspension buffer (5 mL PBS, 50 μL NEB BSA (10 mg/mL), and 25 μL RNase inhibitor (40 U/μL)) ...
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bioRxiv - Cancer Biology 2023Quote: Fresh omentum and omental HGSOC tumor metastasis biopsy samples were cut into small pieces and dissociated in digestion solution (1 mg/mL collagenase/Dispase [Sigma cat. no. 10269638001], 1 unit/mL DNase I [NEB, cat ...
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bioRxiv - Genomics 2023Quote: ... The resulting digested DNA (4 ml in total) was divided into four aliquots and diluted in 8 ml of ligation buffer (1X ligation buffer NEB without ATP ...
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bioRxiv - Cancer Biology 2021Quote: ... using an RNA input of 100 ng per sample (New England BioLabs, Ipswich, USA). Subsequently ...
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bioRxiv - Genetics 2021Quote: ... After digesting 100 ng of DNA from each sample using EcoRI and MseI (NEB), adapters containing barcode sequences were ligated to the ends of the DNA fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin fraction was solubilized in Buffer A with 1:100 micrococcal nuclease enzyme (NEB) and incubated for 15 min at 37°C shaker.
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bioRxiv - Molecular Biology 2020Quote: Tailing and ligation: Plugs were equilibrated once in 100 μl 1x TdT buffer (NEB) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and 100 ng of the sgRNA in NEBuffer 3.1 (New England Biolabs, Ipswich, USA) for 20 minutes at 37°C and 10 minutes at 65°C ...
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bioRxiv - Genetics 2019Quote: ... 10 mM EDTA and then digested with 100 U of NotI (New England Biolabs) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... H2O up to 100 μL) was performed with phi29 DNA polymerase (New England Biolabs) at 30 °C for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of genomic DNA was treated with McrBC enzyme (New England Biolabs, M0272L) at 37°C overnight and then for 10 min at 75°C to deactivate the enzyme ...
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bioRxiv - Plant Biology 2021Quote: ... GST was cleaved using TEV protease (100 μL at 400 μM – New England Biolabs), and GST beads were added to eliminate GST and undigested proteins ...
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bioRxiv - Biochemistry 2021Quote: ... Extra RT primers were removed by Exonuclease I (100 U; M0293S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Plant Biology 2019Quote: ... Amplicon sizes were estimated using 100 bp DNA ladder (New England Biolabs, Ipswich, MA). Genomic DNA of C ...
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bioRxiv - Genetics 2020Quote: ... 60 µg of this 3C-extract were digested with 100 units NcoI-HF (NEB) over night ...
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bioRxiv - Molecular Biology 2019Quote: 2 µg of genomic DNA was digested with 100 units of MspI (NEB R0106M) or TaqαI (NEB R0149M ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was digested overnight (ON) with either 2 μl (100 U) Dpn II (NEB) (later experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of isolated DNA was digested with MspI restriction enzyme (NEB, Ipswich, MA), carried out end-repair/adenylation (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... ∼100 ng of RNA was treated with NEBNext rRNA Depletion Kit (New England Biolabs). RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: 100 μM of compound was incubated with 0.02 units P1 nuclease (New England Biolabs) in P1 reaction buffer (50 mM sodium acetate pH 5.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Biophysics 2023Quote: ... 100 ng or less template DNA and 24 μL Taq DNA polymerase (NEB M0273L). Purify and concentrate the PCR DNA product by ethanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 µg of DNA was incubated with 0.1 U/µL of USER enzyme (NEB) in 500 µL at 37°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... were annealed (81 µl of each Oligo 100 µM, 1x T4 ligase buffer; NEB) at 98 °C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... the DNA was washed once with 100 μl of 1x λ exonuclease buffer (NEB). The DNA bound to the beads was made ss by λ exonuclease digestion of the strand with 5’ phosphate (5 U/mg DNA ...
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bioRxiv - Developmental Biology 2020Quote: ... and XbaI (New England Biolabs, 20U/ml, #R0145S) recognition sequence extensions ...
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bioRxiv - Biophysics 2021Quote: ... and 40 µg/ml BSA (New England Biolabs), adjusted to pH 7.9.
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bioRxiv - Molecular Biology 2021Quote: ... treated with apyrase (25 mU mL−1, NEB) and incubated at room temperature for another one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.2 mg/ml BSA (New England Biolabs). The blocked beads were added to the samples and incubated for 4 hr at 4°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 400 U/µL (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL mg/ mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 5 U/µL (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA Ligase at 5 U/ µL (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... Meantime 20 mL Chitin Resin (NEB cat#S6651L) was equilibrated in a column with 200mL of HEGX buffer supplemented with protease inhibitor and loaded with the supernatant from the PEI-centrifuge step by slow gravity flow (<0.3mL/min) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.5 µL BsaI (10000 U/mL, NEB) and water to bring the final volume to 10 µL ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl BsmBI (10,000 units/ml, NEB, R0580), and water was added to 14 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... into a BbsI (10,000 units/ml, NEB, R0539) digested pCDF4 (50 ...
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bioRxiv - Genomics 2020Quote: ... 2 μl Antarctic Phosphatase [5k U/mL] (NEB) and 1 μl RNase inhibitor and incubating at 37 °C for 30 minutes with shaking ...
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bioRxiv - Neuroscience 2020Quote: ... or α2-3,6,8 Neuraminidase (5 U/ml; NEB) for 2 hours before motoneurons were added (Supplementary Figure 7).
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bioRxiv - Bioengineering 2021Quote: ... in addition to 6μg/mL of Streptavidin (NEB) and 0.125% of rLysozyme (Merck 27–33 kU/μl) ...