Labshake search
Citations for New England Biolabs :
601 - 650 of 3546 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... To generate these constructs gene block fragments that codify for those sequences were produced in the Duke Transgenic Mouse Facility and cloned into the backbone using EcoRI-HF Restriction Enzyme (NEB) and In-Fusion HD Cloning Kit (Takara ...
-
bioRxiv - Molecular Biology 2023Quote: ... The corresponding regions of a partial mouse Azin1 gene were amplified from mouse tail genomic DNA by using Phusion Hot Start Flex 2X Master Mix (New England Biolabs) and the following primers ...
-
bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Immunology 2024Quote: N-linked glycans were enzymatically released from purified mouse THP using PNGase-F kit (catalog no P0709S, New England Biolabs). N-glycans were then purified from the reaction mixture containing denaturing buffer and de-N-glycosylated proteins by solid phase extraction method using Sep-Pak C18 (1 cc Vac-cartridges ...
-
bioRxiv - Microbiology 2024Quote: ... AnTat1.1 sequence was obtained from AnTat1.1 specific cDNA from mouse infection D6 cloned into a pMiniT vector with the PCR Cloning Kit (NEB, E1202S). VSG-228 was partially amplified from VSG PCR (see below ...
-
bioRxiv - Genomics 2023Quote: ... and incubated at 37°C for 1 hour with RNase A/T1 (Thermo, 1/20 volume) and RNase H (NEB, 1/50 volume). The DNA was then purified again.
-
bioRxiv - Neuroscience 2021Quote: ... 1 μL cDNA was amplified using 1 μL Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer with 10 μM primers ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μL Bacillus stearothermophilus (Bst) DNA polymerase (8 U μl-1) (New England Biolabs), 2 μL DNA template ...
-
bioRxiv - Molecular Biology 2020Quote: ... amplicons were visualized on ~1% agarose gels with 1 kb plus DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 μl of High Concentration T4 RNA Ligase 1 (New England BioLabs, M0437)] at 23°C for 5 h ...
-
bioRxiv - Microbiology 2023Quote: ... lysates (1 mg) were incubated with MNase (10 units/1 mg lysate; NEB #M0247S) at 37°C for 60 min ...
-
bioRxiv - Genetics 2023Quote: ... containing 1× Exonuclease I Buffer and 1 U/µL Exonuclease I (NEB, cat # B0293S), and incubated at 37 °C for 45 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1× CutSmart buffer (NEB) to 17 μl ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 x PNK buffer (NEB), and 20 U RiboLock ...
-
bioRxiv - Genomics 2020Quote: ... 1 μl Exonuclease III (NEB), and NEBuffer 1 (NEB ...
-
bioRxiv - Genomics 2019Quote: ... 1 ul Phi29 polymerase (NEB), 34 ul ultrapure water) ...
-
bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (1:40, NEB Murine RNAse Inhibitor or Thermofisher Ribolock ...
-
Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
-
bioRxiv - Bioengineering 2019Quote: ... 1 × Zinc (supplied by NEB), 5 μL of 10 × GlycoBuffer 4 (supplied by NEB ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 μl Klenow exo-(NEB) and varying amounts of α[32P]-dCTP added before incubation for 1-3 hours at 37 °C ...
-
bioRxiv - Synthetic Biology 2019Quote: ... 1× Taq polymerase buffer (NEB) and 2 μM or 20 μM each of the mutagenic nucleotide analogs 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP ...
-
bioRxiv - Genomics 2020Quote: ... 1 µL T4 PNK (NEB) and 6 µL H2O were mixed and incubated in a thermocycler (BioRad ...
-
bioRxiv - Genetics 2019Quote: ... 1× T3 ligation buffer (NEB) and 300 ng total RNA with or without FTO treatment ...
-
bioRxiv - Genomics 2021Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
-
bioRxiv - Genomics 2021Quote: ... 1 μl of DpnI (NEB) was added to the reaction mixture to further incubate at 37 degrees for 10min ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 1.875 U XRN-1 (NEB), 1 mM CaCl2 ...
-
bioRxiv - Genomics 2020Quote: ... 1 µl RppH (5U, NEB)] was added ...
-
bioRxiv - Genetics 2021Quote: ... 1 µl of T7EI (NEB) was then added to 19 µl of purified (QIAquick PCR Purification Kit ...
-
bioRxiv - Genomics 2021Quote: ... 1 μL TET2 (NEB #7120S) and 1 μL T4-BGT (NEB #M0357S ...
-
bioRxiv - Systems Biology 2020Quote: ... 1 μL T4 ligase (NEB), 2 μL T4 ligase buffer (NEB) ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 mM ATP (NEB) in 1x PNK reaction buffer (70 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Cancer Biology 2020Quote: ... T7 Endonuclease 1 (NEB, M0302) was added to the mixture and incubated at 37°C for 15 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 μl NAD+ (NEB, B9007) and 1 μl dNTP (Promega ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 1 U/μl Esp3I (NEB), 0.5 U/μl T4 Polynucleotide Kinase (NEB) ...
-
bioRxiv - Systems Biology 2020Quote: ... 1 U RNase Inhibitor (NEB)) ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μl T4 ligase (NEB) and 1 μl of the relevant restriction enzyme in a total volume of 20 μl made up in dH2O ...
-
bioRxiv - Genomics 2021Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μL PNGase F (NEB), and 5 μL water were added and the solution incubated for 37 °C for 1 h.
-
bioRxiv - Biochemistry 2020Quote: ... 1 µL of Xmn1 (NEB) was added to the DSB sample to generate the DSB ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 µL rSAP (NEB), incubating for 15 min at 37°C then 15 min at 80°C ...
-
bioRxiv - Genetics 2022Quote: ... and T4 ligase 1 (NEB) sequentially ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and 1 μL DpnI (NEB) was added and incubated for 30 min at 37 °C prior to deactivation at 65 °C for 20 min.
-
bioRxiv - Immunology 2020Quote: ... 1 U RNase inhibitor (NEB), 10 mM fresh DTT ...
-
bioRxiv - Biophysics 2019Quote: ... 1 µL T7 polymerase (NEB), 5 mM MgCl2 ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µl T4 PNK (NEB) and 1 µl ATP [γ-32P] 250 µCi (Perkin Elmer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 1 μl dNTP mix (NEB), 0.2 μl MgCl2 ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μl lambda phosphatase (NEB) and 69 μl ddH2O was added ...
-
bioRxiv - Biochemistry 2019Quote: ... 1 U UDG (NEB, M0280S), and 1X UDG buffer (NEB).
-
bioRxiv - Genetics 2020Quote: ... 1 ul T4 PNK (NEB), and 6 ul of water ...