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Citations for New England Biolabs :
351 - 400 of 2232 citations for Rat TMEM165 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Infectious clone plasmids were linearized using AscI (New England Biolabs) and then in vitro transcribed using the MEGAscript SP6 transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: The TALEN encoding plasmids were linearized with NotI (NEB R0189S), which cut once downstream of the TALEN ORF ...
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bioRxiv - Developmental Biology 2022Quote: Plasmid with iTol2 sequence linearized using Not1 restriction enzyme (NEB). Linearized DNA was used as a template and RNA synthesis was performed using the HiScribe SP6 RNA Synthesis Kit (NEB ...
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bioRxiv - Immunology 2022Quote: ... The resulting pAF300 plasmid was linearized with PmeI (NEB, R0560S) and then transformed into Escherichia coli BJ5183 cells containing the E1/E3-deleted pAdEasy Ad5 vector backbone (Figure 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... the pCS2-zswim5 plasmid was linearized with NotI-HF (NEB) and purified using a Wizard SV Gel and PCR Clean-up System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The parent plasmid was treated with BamHI (NEB, Ipswich, MA) and EcoRI (NEB ...
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bioRxiv - Biophysics 2021Quote: ... were produced by linearization of large plasmids with NotI (NEB). Without further purification ...
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bioRxiv - Bioengineering 2020Quote: Plasmids were linearized with Not-I HF (New England Biolabs) overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: pUC19 plasmid was digested with SmaI (New England Biolabs, R0141S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Immunology 2022Quote: ... plasmid 17398) using Gibson Assembly Master Mix (New England BioLabs). After sequence validation by Sanger sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... plasmids were digested with BamHI-HF (New England Biolabs R3136S)/MfeI-HF (New England Biolabs R3589S ...
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The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019Quote: All plasmids were cloned using Gibson assembly (HiFi Assembly, NEB) using DNA amplified from P ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Ligated plasmids were transformed into BL21 (DE3) cells (C2527, NEB) and correct clones verified by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: Tol2 transposase plasmid (pCS-TP) was linearised using Not1 (NEB) and mRNA was transcribed in vitro using the mMESSAGE mMACHINE SP6 Kit (Ambion Inc.) ...
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bioRxiv - Molecular Biology 2021Quote: ... These plasmids were linearized with Pme I (New England Biolabs) and transformed in strain DPY034 for integration at the TRP1 locus to obtain strains DPY1805 and DPY1818 ...
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bioRxiv - Microbiology 2021Quote: ... The N expression plasmid was linearized using EcoRV-HF (NEB). Capped-RNA was produced using the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NheI (New England BioLabs) between the end of KDM5B and the MS2 stem loops ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NheI (New England BioLabs) between the end of KDM5B and beginning of SunTag ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NotI (New England BioLabs) at the beginning of SM ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with AscI (New England BioLabs) between the end of Kif18b and beginning of SM ...
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bioRxiv - Cell Biology 2020Quote: The parental plasmid was digested with the DpnI enzyme (NEB). The mCherry was then replaced by eGFP to generate the RUSH CD63-YA-eGFP plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... Whole-plasmid amplification reactions were performed using Phusion polymerase (NEB) and Ampligase (Epicentre) ...
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bioRxiv - Synthetic Biology 2021Quote: ... treatment and DNA purification with Monarch Plasmid Miniprep kit (NEB), or (ii ...
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bioRxiv - Microbiology 2020Quote: ... replicon plasmid DNA linearized with AscI (NEB, Ipswich, Massachusetts, USA) was used to template T7 in vitro transcription reactions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid construction was carried out using enzymes obtained from NEB (NEBuilder HiFi DNA assembly master mix ...
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bioRxiv - Microbiology 2021Quote: ... of existing plasmids or HiFi Gibson Assembly (New England Biolabs) of linear dsDNA fragments ...
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bioRxiv - Genetics 2020Quote: ... ESCs were electroporated with the linearized plasmid (Fsp1 NEB R0135) in the presence of hygromycin and individual clones were isolated as we have done previously and expanded for further experiments.
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bioRxiv - Genetics 2020Quote: ... Ligated plasmids were transformed into NEB High Efficiency (NEB, C2987) bacteria ...
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bioRxiv - Molecular Biology 2021Quote: ... The pLentiGuide-Blast plasmid was digested with BsmBI-V2 (NEB) at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: pUC19 plasmid was digested with SmaI (New England Biolabs, R0141S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Microbiology 2022Quote: ... The minipreped plasmids were cut by HindIII (NEB cat. R3104S), and gel electrophoresed.
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bioRxiv - Plant Biology 2022Quote: ... and introduced into binary plasmids using NEBuilder Hifi assembly (NEB) (Figure 1a) ...
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bioRxiv - Immunology 2022Quote: ... plasmids were linearized with PstI (New England Biolabs, MA, USA) and purified using a PCR purification kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Molecular Biology 2023Quote: ... The parental plasmid was digested with 5U DpnI (NEB, R0176S). Plasmid transformation was achieved by using the heat shock method (42°C ...
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bioRxiv - Bioengineering 2023Quote: ... The Csy3-VPR-Csy4 plasmid was digested with MluI (NEB) and BamHI (to remove the VPR TAD ...
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bioRxiv - Cell Biology 2023Quote: ... we linearized the plasmid for genomic integration using AflII (NEB), a silent mutation inserted previously.
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bioRxiv - Immunology 2023Quote: ... plasmids using T4 DNA ligase (New England BioLabs, cat. M0202S). Sanger sequencing confirmed successful cloning.
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bioRxiv - Biophysics 2023Quote: ... We digest the plasmid with Hind III (New England Biolabs) to generate a linear template ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmids were verified by digestion by restriction endonucleases (NEB). Plasmid mapping and sequencing alignments were carried out using SnapGene software (GSL Biotech ...
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bioRxiv - Genomics 2023Quote: ... 46 plasmid using restriction digestion with AatII and Aflll (NEB). The library was then re-electroporated and purified as above.
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bioRxiv - Biophysics 2023Quote: ... Plasmids were linearized using Nhe I HF (New England Biolabs) and transcribed in vitro (mMessage-mMachine T7 Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... The final donor plasmid was generated through Gibson cloning (NEB) of a synthetic FseI-HA-KRAB-AscI DNA fragment (GenScript ...
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids bearing mutations were cut with AseI (NEB, Catalog # R0526S) and SspI (NEB ...
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bioRxiv - Genomics 2023Quote: ... Dual-enSERT-2.1 plasmid was digested with NotI (NEB, R3189) and AgeI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... the plasmids were linearized using EcoRV endonuclease (New England Biolabs). cRNA synthesis was accomplished through in vitro transcription using the HiScribe T7 ARCA mRNA Kit with tailing (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... Gel extraction and plasmid preparation kits were purchased from NEB and Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: We digested the resulting plasmids by NotI-HF® (NEB) and inserted cassettes expressing fusions of antibiotic resistance genes with deltaTK downstream of the lox66 sites by Gibson assembly ...