Labshake search
Citations for New England Biolabs :
501 - 550 of 9852 citations for Rat Heat Shock Protein Beta 8 HSPB8 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... 1μl of T4 bacteriophage protein (NEB, 5ng/μl), 3μl of DMSO ...
-
bioRxiv - Molecular Biology 2019Quote: ... The protein was purified using amylose resin (NEB) using the provided protocol ...
-
bioRxiv - Pathology 2020Quote: ... plasma and histone H3 protein (New England Biolabs) as standard were subjected to SDS-PAGE and electrically transferred onto PVDF membrane (Millipore) ...
-
bioRxiv - Biophysics 2020Quote: ... Eluted protein was deglycosylated with PNGase F (NEB) by incubating 5 units of the enzyme per 1 μg of protein for 2 h at 37 °C under gentle agitation ...
-
bioRxiv - Developmental Biology 2020Quote: ... The gRNAs were mixed with protein Cas9 (NEB), allowed 5 minutes at 37°C to assemble into ribonucleoprotein complexes ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 μL Protein G magnetic beads (NEB, S1430S) were used ...
-
bioRxiv - Cell Biology 2019Quote: ... The gRNA and Cas9 protein (New England Biolabs) were injected into one-cell stage NHGRI-1 embryos in a 2 nl volume consisting of ∼50 ng/μl gRNA and ∼300 nM Cas9 ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to 1x protein kinase buffer (NEB) supplemented with 200 μM “cold” ATP (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... Ribonucleoprotein complexes of SpCas9 protein (NEB; Ipswitch, MA), ssODN template and gRNA were prepared with final concentration 200 μg/μl ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and Broad Range Protein Marker (New England Biolabs).
-
bioRxiv - Microbiology 2023Quote: ... coli protein synthesis system (New England BioLabs inc.). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... 400 units mL-1 lambda protein phosphatase (NEB), and 0.1 μg mL-1 protein phosphatase 2a (Cayman Chemical ...
-
bioRxiv - Cell Biology 2023Quote: ... and PMP with Lambda Protein Phosphatase (P0753S, NEB) with Phosphatase inhibitor cocktail (4X ...
-
bioRxiv - Molecular Biology 2023Quote: ... The proteins were purified by Amylose resin (NEB) and eluted with 10 mM maltose ...
-
bioRxiv - Biophysics 2023Quote: ... and phosphorylated with protein kinase A (PKA) (NEB) for 1 hour at 20 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 µL of Lambda Protein Phosphatase (NEB, P0753S) was added to aliquots 3 and 4 (PPase only) ...
-
bioRxiv - Biochemistry 2024Quote: ... 30nM sgRNA and 30 nM Cas9 protein (NEB) for 1h at room temperature and analyzing cleavage products on a 2% agarose gel ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2 µL of λ protein phosphatase (NEB P0753S) was added and incubated at 37°C for 30 minutes and then placed on ice ...
-
The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... coli protein synthesis system (PURExpress, New England Biolabs). Reactions were performed according to manufacturer’s instruction and as previously described in 82 with a few modifications ...
-
bioRxiv - Microbiology 2020Quote: ... Cloning and purification of full length IE86 protein: Full length IE86 protein was codon optimized and cloned in pMALcx5 vector (New England Biolabs Inc.). Cells were transformed in BL-21 cells and IE86-FL-pMALcx5 transformed cells were grown in 1L luria broth (Thermofisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: ... Phosphorylation of purified LPR1 protein was tested according to (Maldonado-Bonilla et al., 2014) using λ-protein phosphatase (New England Biolabs). In brief ...
-
bioRxiv - Genetics 2022Quote: ... along with 6uL of EZ-Run Pre-Stained Rec Protein Ladder (Fisher BP3603-500) and 3uL of Blue Prestained Protein Standard (NEB P7718S). Gels were electrophoresed in XT Tricine Running Buffer (Bio-Rad 1610790 ...
-
bioRxiv - Systems Biology 2023Quote: ... To remove any remaining phosphorylation the GFP-Trap bound proteins were treated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
-
bioRxiv - Microbiology 2021Quote: ... The protein was buffer exchanged into enterokinase cleavage buffer (20 mM Tris pH 8, 50 mM NaCl, 2 mM CaCl2) and cleaved using bovine enterokinase (EK, NEB) at 16U/mg protein for 4 hrs ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples with RNA Integrity Number > 8 were used for library preparation following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Library construction and sequences was carried out at the Biomedical Research Centre Sequencing Facility (University of British Columbia ...
-
bioRxiv - Molecular Biology 2020Quote: ... PEARL extracts from cultured cells were treated with either DNase I (1 unit per every 8 μL of PEARL extract, New England BioLabs) or with RNase A (0.1 mg per every 8 μL of PEARL extract ...
-
bioRxiv - Molecular Biology 2021Quote: ... after incubating the samples with 2 μl of RNase A (Thermo) at 37 °C for 2h and then with 8 μl of proteinase K (NEB) at 55 °C for 4h ...
-
bioRxiv - Cell Biology 2022Quote: ... In-house adapters containing 8-nucleotide unique molecular identifier (UMI) sequences were ligated onto the DNA fragments with T4 DNA ligase (NEB). Excess adapters were removed by AMPure XP beads (Agencourt ...
-
bioRxiv - Genomics 2019Quote: The circularized DNA was split into 8 50 ul rolling circle amplification (RCA) reactions (5 ul 10x Phi29 buffer (NEB), 2.5 ul 10 mM dNTPs (NEB) ...
-
bioRxiv - Genomics 2019Quote: ... We quantified DNA using a Qubit dsDNA BR assay and performed a debranching treatment on 8-10 µg of DNA in 100 µL reactions containing 50 Units of endonuclease T7E1 (New England Biolabs) in 1X NEB buffer 2 ...
-
bioRxiv - Genetics 2019Quote: The mutations comprising the pol30-8 allele (R61A, D63A) were introduced into pEAA578 (POL30∷KANMX) by Q5 site-directed mutagenesis (New England Biolabs) to create the single step integrating vector pEAA633 (pol30-8∷KANMX) ...
-
bioRxiv - Microbiology 2021Quote: ... and Bakt_805R (GACTACGVGGGTATCTAATCC)38 PCR primer pair with an individual 8 bp barcode adapter (based on the NEB Multiplex Oligos for Illumina, New England Biolabs) attached to the forward primer and the reverse primer ...
-
bioRxiv - Molecular Biology 2020Quote: ... The converted RNA was fragmented into 150 - 200 nt fragments by incubation at 94 °C for 8 minutes in fragmentation buffer (NEB). The fragmented RNA was then used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4.8 mM MgCl2 and 1x reaction buffer for T7 RNAP and 62.5 units of T7 RNAP (New England BioLabs, NEB). The mixture was incubated for 2 h at 37°C.
-
bioRxiv - Molecular Biology 2020Quote: ... 4.8 mM MgCl2 and 1x reaction buffer for T7 RNAP and 62.5 units of T7 RNAP (New England BioLabs, NEB). The mixture was incubated for 2 h at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then ligated to pre-annealed double-stranded adaptors that contain single dT overhangs and a unique molecular identifier (UMI) consisting of a randomized 8-base sequence containing a 3-base specific barcode by T4 DNA ligase (NEB) overnight at 15 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 µL of the Klenow reactions were retrieved and added to 8 µL of 2x RNA Loading Dye (New England Biolabs). The mixtures were then analyzed by electrophoresis through a urea-15% polyacrylamide gel (Novex TBE-Urea gel 15% ...
-
bioRxiv - Genomics 2022Quote: ... Another half of the genomic DNA (less than 8 ng/μL) was treated with 0.2 unit/μL MseI (NEB, R0525S) for 1 hour at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5ul of PCR1 product as template was amplified using unique i5 and i7 index primer combinations with 8 cycles and Q5 High-Fidelity DNA Polymerase (NEB) for each individual sample to allow pooling of sequencing libraries ...
-
bioRxiv - Molecular Biology 2021Quote: ... 8 μg of digested nucleic acids were treated or not with 10 μl of RNase H (New England BioLabs, M029L) overnight at 37 °C in 1x RNAse H buffer and 1/10 of the samples were used as input ...
-
bioRxiv - Molecular Biology 2020Quote: ... Approximately 8 ug of <200nt RNA per sample was mixed to an equal volume of 2x loading dye (NEB #B0363A) and incubated at 70°C for 5min ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Chromatin was then digested by adding 12 μl of 10x NEBuffer3.1 and 8 μl of 5 U/μl DpnII (NEB, R0543) followed by a 2h incubation at 37ºC in a ThermoMixer with shaking (900 rpm ...
-
bioRxiv - Immunology 2022Quote: ... 2018” tagmentation mix were either sorted into plates containing RCB buffer for condition “hiSDSprotK-TWEEN” (2 x RCB: 100 mM Tris-HCl pH 8, 100 mM NaCl, 40 µg/mL Proteinase K (NEB), 0.4 % SDS (Sigma ...
-
bioRxiv - Biophysics 2019Quote: ... 10 μl of 100 μM DNA oligos containing an amino group modification at their 5’ends were mixed with 8 μl of 20 mM BG-GLA-NHS (NEB) in 40 μl of 50 mM HEPES buffer containing 50% anhydrous DMSO ...
-
bioRxiv - Biophysics 2019Quote: ... gels were gently removed from the chamber and digested overnight at 37 °C in 8 units mL−1 Proteinase K (NEB) diluted in digestion buffer (1× TAE buffer ...
-
bioRxiv - Physiology 2019Quote: ... Qualifying samples (samples with RNA integrity numbers > 8) were then prepared following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 mM CaCl2 was added and the His8 tag was cleaved through the addition of 8-16 U/mL enterokinase (New England Biolabs) for 2 days at 37°C ...
-
bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs] ...
-
bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
-
bioRxiv - Genomics 2021Quote: ... Fragmentation was carried out by adding 50 μL NEB Buffer 2 and 8 μL of 25 U/μL MboI restriction enzyme (New England Biolabs). Samples were incubated at 37 °C for 2 hours with rotation ...