Labshake search
Citations for New England Biolabs :
151 - 200 of 10000+ citations for Rat Heat Shock 60 kDa Protein 1 Chaperonin HSP60 HSPD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: Fetuin was deglycosylated under denaturing conditions using Protein Deglycosylation Mix II kit (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... PhyR1 and PhyR2 proteins were overexpressed and purified using the IMPACT kit (New England Biolabs) following the manufacturer’s instructions and equal procedures for the four of them ...
-
bioRxiv - Molecular Biology 2019Quote: ... in vitro translation was performed using the PURExpress In vitro Protein Synthesis kit (NEB 6800) and the non-stop ribosome complex was purified as previously described in ref.38 ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
-
bioRxiv - Biophysics 2022Quote: Plasmids expressing stdMCP-fusion proteins were generated using the NEBuilder HiFi DNA Assembly kit (NEB). To obtain plasmids carrying an stdGFP-tagged stdMCP gene ...
-
bioRxiv - Cell Biology 2021Quote: ... 60 U micrococcal nuclease (MNase, New England Biolabs, 1U/µL in MNAse buffer) was added and cells were incubated at 37 °C for 5 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... and lysate was incubated 37°C 60 min in 2 units CIP (NEB) per 50 μL reaction containing 50μg of total protein ...
-
bioRxiv - Microbiology 2020Quote: ... in 0.5 x TBE for 60-90 min alongside 100 bp ladder (NEB). Gels were stained for 30 minutes in ethidium bromide solution before imaging under UV-light.
-
bioRxiv - Bioengineering 2022Quote: ... for 60 min at 37 °C and proteinase K (New England Biolabs, P8107S) for 3 hours at 55 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 60 μg of plasmid DNA was linearized with AhdI (New England Biolabs, UK) and extracted with phenol-chloroform for injection.
-
bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
-
bioRxiv - Immunology 2020Quote: ... and on the Sprague Dawley (SD) strain (rats) (Hera Biolabs) were obtained from vendor and breed in the Division of Laboratory Animal Resources (DLAR ...
-
bioRxiv - Microbiology 2023Quote: ... plasmid DNA encoding rat HEV was linearized using EcoRI (NEB). Five percent of the reaction was subjected to gel electrophoresis with ethidium bromide staining and visualized with ultraviolet light to verify that linearization had occurred.
-
bioRxiv - Molecular Biology 2024Quote: ... The 20-75 kDa region was excised and digested with proteinase K (New England Biolabs). RNA was isolated with acid phenol/chloroform/isoamyl alcohol (pH 6.5 ...
-
bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
-
bioRxiv - Systems Biology 2020Quote: ... MnCl2 to a concentration of 1 mM and NEBuffer for Protein MetalloPhosphatases (NEB) diluted to a 1X concentration were added to both tubes ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF) and proteins were purified by amylose beads (New England Biolabs) and eluted in wash buffer (20mM Tris pH:7.6 ...
-
bioRxiv - Bioengineering 2021Quote: 25 µl reactions were set up following the PURExpress protocol (In Vitro Protein Synthesis Kit, NEB) in black low-volume 384-well polystyrene plates (Corning) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein and non-incorporated nucleotides were removed using the Monarch RNA Clean up kit (NEB, T2040), before quantification by nanodrop.
-
bioRxiv - Microbiology 2024Quote: ... thermophilus extracts or PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the reactions were further incubated at either 50 °C or 37 °C for another 4 h in the case of T ...
-
bioRxiv - Systems Biology 2024Quote: ... at 16 °C overnight, followed by heat-inactivation (65°C, 15 min) and linearization using SbfI-HF (NEB) at 37°C for 2 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chromatin was isolated and then fragmented with 60 units MNase (New England Biolabs, M0247S) at 37 °C for 10 minutes ...
-
bioRxiv - Genetics 2020Quote: ... 60 µg of this 3C-extract were digested with 100 units NcoI-HF (NEB) over night ...
-
bioRxiv - Molecular Biology 2023Quote: ... Digestion of DNA was performed using 60 units of restriction enzyme AseI (NEB, R0526M) per plug ...
-
Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... proteins (2 mg mL-1) were incubated with 180000 U mL-1 PNGase F (New England Biolabs, Unites States) in PBS (pH 7.4 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 μM Illumina barcode (NEB-kit) to 23 μl of cDNA ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... The reaction was heat-inactivated and DNA fragments were self-ligated using T4 DNA ligase (New England Biolabs, M0202T). E ...
-
bioRxiv - Neuroscience 2020Quote: ... incubated for 10 min at 30°C) or heat-inactivated (65°C for 20 min) as described in the supplier’s manual (New England Biolabs).
-
bioRxiv - Molecular Biology 2023Quote: ... selected RNAs from three independent experiments was heat denatured and treated with 0.5 units of Xrn1 (New England Biolabs) for 1 h at 37 °C ...
-
bioRxiv - Biochemistry 2019Quote: ... The assay was performed using the PURExpress In Vitro Protein Synthesis Kit (E6800S New England Biolabs, Inc.), following manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2022Quote: The TF samples were expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
-
bioRxiv - Genomics 2022Quote: ... The TF sample was expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
-
bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
-
bioRxiv - Biophysics 2020Quote: ... concentrated in a 100 kDa concentration device (Amicon) to ~1.5 mg/ml and incubated with EndoHF (NEB) at a volume to volume ratio of 1:20 for 2 hours to remove the N-linked glycan of EWI-FΔIg1-5 ...
-
bioRxiv - Microbiology 2019Quote: ... the pSAG1:U6-Cas9:sgUPRT vector [60] was modified by Q5 site-directed mutagenesis (NEB) to specify sgRNAs targeting ROP17 (F2) ...
-
bioRxiv - Biochemistry 2020Quote: ... Nicking was performed according to the manufacturer’s instructions for 60 min at 50 °C (NEB) and the product was purified using a PCR purification kit (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... 72°C for 60 seconds) in 50 µl reactions using Phusion PCR polymerase mix (NEB) containing 25 pmol each of the following ultramers (the protospacer-matching sequence is underlined) ...
-
bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs) plus 1mM MnCl2 ...
-
bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in 1 volume of buffer A / 300 mM NaCl / 2 mM MnCl2 / 1 mM DTT and incubated with λ protein phosphatase (NEB) at 50 U / mL for 1 hour at 23°C with agitation ...
-
bioRxiv - Biochemistry 2019Quote: ... The template strand was circularized by heat-annealing the ligation splint and subsequently incubating this partial duplex with 200 U Taq DNA ligase (NEB) per 100 pmol of template in Taq DNA ligase buffer for 3h at 45°C ...
-
bioRxiv - Biophysics 2023Quote: ... The 38 kbp T7A1-parS + handle construct were subsequently digested for 1 h at 37°C and heat-inactivated for 20 min at 65°C using SrfI restriction enzyme (New England Biolabs).
-
bioRxiv - Molecular Biology 2023Quote: ... the remaining 5’-phosphate groups were dephosphorylated using calf-intestinal alkaline phosphatase for 10 minutes at 37°C and finally heat inactivated for 20 min at 80°C (Quick CIP, New England Biolabs). The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cosmids were then digested for 2 hours at 37°C and heat-inactivated for 20 minutes at 80°C using SpeI-HF (New England Biolabs). Next ...
-
bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2023Quote: ... Selected candidates were expressed in vitro using the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...