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Citations for New England Biolabs :
151 - 200 of 2232 citations for Rat CPSF7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Individual plasmids were linearized by PmeI (NEB, R0560), 180 bp sequence were amplified to add 10 bp barcodes and additional adapter sequences using a 6 cycles of PCR reaction in 20 μL volume containing 10 μL of Q5 NEBNext Master Mix and 0.5 μM forward and reverse primers (IDT ...
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bioRxiv - Genomics 2020Quote: ... the plasmid was digested with EcoRI (NEB R0101) and gel purified.
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bioRxiv - Neuroscience 2019Quote: ... Plasmids were linearized with PauI (New England Biolabs) restriction enzyme at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... digested plasmid pSL2680 by T4 DNA ligase (NEB), resulting in the gRNA-cassette editing plasmids ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we treated the plasmids with Antarctic phosphatase (NEB) to minimize their self-ligation ...
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bioRxiv - Synthetic Biology 2021Quote: ... isolated using the Monarch Plasmid preparation kit (NEB) and verified by sequencing (Source Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmid was then linearised with NheI (NEB) (loxa_e1 ...
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bioRxiv - Microbiology 2021Quote: ... All plasmids were amplified in Turbo cells (NEB) and isolated using a high purity plasmid prep kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... the pKPL_1 plasmid was linearized with EcoNI (NEB) and SapI (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid was transformed into BL21 (DE3) bacteria (NEB). A single colony was inoculated into 10Ml Terrific Broth (TB ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid was transformed into 10-beta cells (NEB) and clone sequence verified by sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... plasmids were linearized using the NotI-HF (NEB) restriction site and transcribed in vitro using the mMessage mMachine SP6 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and Monarch plasmid miniprep kit (New England BioLabs) used for plasmid purification ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid miniprep was digested with EcoR1 (NEB) and the insert was cloned in pSW23T ...
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bioRxiv - Neuroscience 2021Quote: All plasmids were transformed into NEB Stable (NEB) competent cells for amplification ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmid was digested with Esp31 (NEB, UK), removing a 2kb stuffer.
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bioRxiv - Cell Biology 2020Quote: ... Donor plasmids were generated through Gibson assembly (NEB) of PCR-amplified open reading frames of the desired genes ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmid was with HpaII methyltransferase (NEB, M0214S) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... BAC plasmids were linearised using AgeI-HF (NEB). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... pEC9 plasmid was linearized using Sal1 (NEB R3138). Purified linear DNA was then used to in vitro transcribe EMCV plus stranded RNA using MEGAscript™ T7 Transcription Kit according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The resulting plasmids were incubated with DpnI (NEB) for 1 hr at 37°C and transformed into 5-alpha competent E ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids were digested with AfeI (New England Biolabs) and gel purified to remove the cut IDR fragment ...
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bioRxiv - Immunology 2022Quote: Mutational plasmid libraries were digested using EcoRI (NEB) and NotI (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were digested with BbsI restriction enzyme (NEB) and annealed with double-stranded sgRNA oligos using QuickLigase (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were digested with SpeI (NEB Biolabs; R3133S) and purified by phenol/chloroform extraction ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were then treated with 5U DpnI (NEB) for 30 min at 37°C to degrade any non-replicated plasmids ...
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bioRxiv - Molecular Biology 2021Quote: ... The template plasmids were digested by DpnI (NEB) treatment at 37°C for 1.5 hour ...
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bioRxiv - Synthetic Biology 2019Quote: ... Expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Genomics 2021Quote: ... Plasmids were linearized using PvuI-HF (NEB, R3150L). Linearized plasmids were further purified with phenol chloroform extraction and ethanol precipitation ...
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bioRxiv - Genetics 2020Quote: ... The plasmid was linearized using BsmBI digestion (NEB) and purified using gel extraction (QIAquick Gel Extraction Kit) ...
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bioRxiv - Genetics 2021Quote: ... 50946 plasmid was cut by BbsI (NEB, R3059L) and BamHI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... pastoris plasmids by Q5 site-directed mutagenesis (NEB) using primers Q343N_SDM_WT_D1_D3_F and Q343N_SDM_R.
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bioRxiv - Microbiology 2021Quote: ... Gibson assembly was used for plasmid construction (NEB). amyE gene encoding for α-amylase was amplified from genomic DNA of B ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were cut with restriction enzymes from NEB. Linearized plasmids were re-ligated using T4 DNA ligase (NEB M0202) ...
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bioRxiv - Genetics 2022Quote: ... The resulting plasmids was linearized with HindIII (NEB) and used to synthesize sgRNA using the T7 MEGAscript kit (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... EV71 replicon plasmid was linearized using XhoI (NEB) as previously described75 before in vitro transcription using the T7 RiboMAX system (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... the plasmid was linearized using ScaI (NEB, R3122L). Meanwhile ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were digested with SpeI (NEB Biolabs; R3133S) and purified by phenol/chloroform extraction ...
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bioRxiv - Synthetic Biology 2022Quote: ... before plasmid purification using a miniprep kit (NEB). Error-prone PCR was performed using the GeneMorph II EZClone Domain Mutagenesis kit (Agilent ...
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bioRxiv - Genomics 2022Quote: ... pT3Ts-nCas9n plasmid was linearized with XbaI (NEB), treated with RNAsecure (ThermoFisher Scientific ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The resulting plasmid was digested with BsmBI (NEB) and ligated with the annealed gRNA ...
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bioRxiv - Biochemistry 2023Quote: The plasmid backbones were prepared by SfiI (NEB) restriction of the pSBtet-Hygro vector (Addgene ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were digested with restriction enzymes from NEB and ligated using T4 DNA ligase (NEB M0202) ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were transformed into NEB5α cells (C2987; NEB), prepared using the QIAprep Miniprep Kit (27104 ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... each plasmid digested with BsaI or BsmbI (NEB), and inserts ...
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bioRxiv - Biophysics 2023Quote: ... into plasmid pIA14636 linearized with HindIII (NEB # R3104S) of the fragment containing the mutated origin amplified from gBlock gene fragment (IDT)pGC21823 with primers TL-961 and TL-964 ...
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bioRxiv - Bioengineering 2023Quote: Plasmids were constructed by Gibson Assembly (NEB, E2611L), T4 ligation (NEB ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were generated by Gibson assembly (NEB) using standard protocols or by inverse PCR ...
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bioRxiv - Plant Biology 2023Quote: ... Positive clones were used for plasmid miniprep (NEB) and verified by sanger sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... Positive clones were used for plasmid miniprep (NEB) and verified by sanger sequencing ...