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Citations for New England Biolabs :
551 - 600 of 2232 citations for Rat CPSF7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Plasmid cloning was carried out using NEBuilder HIFI DNA assembly (NEB). Primer sequences are given in Table 1 ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was linearized using BAMHI-HF (New England Biolabs, USA) following manufacturer protocol ...
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bioRxiv - Bioengineering 2024Quote: The glr-1p::GCaMP6s plasmid was made by LR recombination (NEB) of a destination vector containing the DNA sequences for GCaMP6s (Chen et al. ...
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Microbiology 2019Quote: ... in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs, Ipswich, MA, USA) and the MICROBExpress kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Plant Biology 2020Quote: ... Unmodified plasmids were digested with methylation-sensitive enzyme DpnI (New England Biolabs). The mutated plasmids were transformed in E ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into BsaI-restricted PGEM 601 plasmids using T4 ligase (NEB). Ligation product was transformed into E ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into KpnI-BamHI-digested pFL38 plasmid by T4 ligase (NEB). The AAC2 null strain was transformed with pFL38-AAC2-flag ...
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bioRxiv - Molecular Biology 2019Quote: ... Unmethylated control plasmid was produced in dam− bacteria (New England BioLabs #C2925H) and methylation status was confirmed by DpnI and DpnII digestion ...
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bioRxiv - Genetics 2022Quote: ... The linearised plasmid was blunted using T4 DNA polymerase (New England Biolabs) and re-ligated.
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bioRxiv - Neuroscience 2021Quote: ... pX330 vector (addgene plasmid #42230) was digested with BbsI (NEW ENGLAND BIOLABS), and each sgRNA sequence was cloned into the sgRNA cloning site as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified plasmids were linearized by overnight digestion (37°) with EcoRI-HF (NEB) and the products were then visualized on 1% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid constructs were modified by site-directed mutagenesis (E0554; New England Biolabs) to create the desired mutations in klp-6 and cwp-4 ...
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bioRxiv - Molecular Biology 2021Quote: PHO4 was cloned into the 2micron plasmid pRS424 using Gibson assembly (NEB). pGAL-H3-3xHA-URA3 on a single copy plasmid is a gift from the Michel Strubin lab.
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bioRxiv - Molecular Biology 2020Quote: ... After elimination of original plasmid template by digestion with DpnI (NEB R0176), amplification products were transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... The plasmid was simultaneously digested with restriction endonucleases BsaI and BstXI (NEB). The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit) ...
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bioRxiv - Cell Biology 2022Quote: ... the LentiGuide-Hygro plasmid was digested using BsmBI-v2 enzyme (NEB #R0739), and the larger band (~9 kb ...
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bioRxiv - Molecular Biology 2021Quote: ... The resultant plasmids were digested with PacI and PmeI (New England Biolabs) and used to transfect L ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L). NEB 5-alpha Competent E ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into the control plasmid using restriction enzyme XhoI (NEB, #R146L). pCAGGS-Cre plasmid was generated as described (Kranz et al. ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid prib(+)XpA was digested by NruI and SnaBI (New England Biolabs) and ligated to produce prib(+)XpA lacking the poliovirus capsid-encoding region from 1175 to 2956 (prib(+)XpAΔ-1175-2956).
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bioRxiv - Molecular Biology 2019Quote: 50 µg of the plasmid DNA were cut with HindIII-HF (NEB) in Cut Smart buffer with 100 U of enzyme for 1.5 hours at 37°C in 50 µl ...
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bioRxiv - Genomics 2019Quote: ... The 3XFlag-pA-Tn5-Fl plasmid was transformed into C3013 cells (NEB) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... dP75 plasmid with mutations were generated by Site-Directed Mutagenesis Kit (NEB). For yeast two-hybrid assays ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... expression plasmids pETgshA and pETgshB were assembled using HiFi Assembly MasterMix (NEB), and transformed into chemically competent E ...
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bioRxiv - Genomics 2021Quote: ... and cloned into plasmid pT2 using Gibson Assembly Cloning Kit (NEB, E5510S). Obtained pT2_FF plasmids were used as templates for PCR-based barcoded library preparation (22).
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Microbiology 2020Quote: The plasmid pAAV S1-Fc was digested with New England Biolabs (NEB) Restriction Enzymes Pvu I-HF (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Cas9 and dCas9 plasmids were linearized with the PacI restriction enzyme (NEB) to enable plasmid integration at the NEUT5L locus ...
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bioRxiv - Microbiology 2022Quote: ... The linear plasmid pCAG-MYOC cut by the BstEII restriction enzyme (NEB) was purified ...
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bioRxiv - Molecular Biology 2022Quote: About 3 μg plasmid was digested with 20 units SapI (NEB, R0569) and dephosphorylated with 3 units rSAP (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... All fragments for plasmids cloning were amplified using Q5 DNA Polymerase (NEB) and the plasmids were assembled by isothermal assembly ...
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bioRxiv - Microbiology 2022Quote: ... plasmids were linearized by incubation overnight with MfeI (McMillan; New England Biolabs) or NotI (IMP181 ...
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bioRxiv - Plant Biology 2022Quote: ... The plasmid was digested with XhoI and XbaI (New England Biolabs, UK). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Laconic sensor mRNA was synthesised from pCS2 plasmids linearised with NotI (NEB), with mMESSAGE mMACHINE SP6 Transcription Kit (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... and ligated into the preferred plasmid using Quick ligase (New England BioLabs). The plasmids used in this study are listed in Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... The plasmid was linearized by restriction digest with PmeI and SbfI (NEB) and incorporated into the genomes of CM018 and Kn99a by biolistic transformation (Chun and Madhani 2010) ...
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bioRxiv - Synthetic Biology 2020Quote: ... before being ligated to plasmid vectors using T4 DNA Ligase (NEB, M0202). NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: Mutated plasmids were digested with BamHI restriction enzyme (NEB, Ipswich, MA, USA), purified and used for in vitro transcription reaction as described above ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting plasmid was digested with NotI and KasI (New England Biolabs) to remove a truncated kanR gene upstream of arif-1 ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids expressing sgRNAs were cloned using T4 DNA Ligase (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... through ligation of both plasmids after digestion with BamHI (NEB, Cat# R313614) and BspEI (NEB ...
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bioRxiv - Synthetic Biology 2019Quote: ... Prepared plasmid DNA was linearized using SmaI (New England Biolabs, Ipswich, MA) digestion for 4 hours at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... Each probe was amplified from the corresponding plasmid using either OneTaq (NEB) or Taq polymerase (Roche) ...
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bioRxiv - Synthetic Biology 2021Quote: ... digested pU6 plasmid backbone using 1μl of T4 PNK (NEB cat #M0201L) and 1μl of T4 ligase (NEB cat #M0202L ...