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Citations for New England Biolabs :
151 - 197 of 197 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The 5’-terminus was capped with a type I 7-methylguanosine cap (m7G) using the Vaccinia Capping System (NEB, M2080S) and 2’-O-methyltransferase (NEB ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... coli C2523 pMAL-c5X vector and recombinant MUP (rMUP) was made using pMAL Protein Fusion and Purification System (New England Biolabs) using methods similar to prior studies27,36 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplifications were performed in an Applied Biosystems 7500 Fast Real-Time PCR System using the Luna Universal Probe Mix (NEB). We evaluated the performance of the qPCR primers and probes using a DNA concentration gradient spanning 0.1-100.0 ng/reaction in single gene reactions as well as in a multiplex reaction ...
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bioRxiv - Plant Biology 2021Quote: The MBP-NbERF-IX-33a fusion protein was prepared using the pMAL Protein Fusion and Purification System (New England Biolabs). E ...
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bioRxiv - Biochemistry 2021Quote: ... using a plasmid that codes for the desired protein sequence fused to an N-terminal intein and chitin binding domain (CBD) as part of the IMPACT purification system (NEB). 2 liters of cells were grown to an OD600 of 0.6 and induced with 1 mM IPTG for 3 hours at 25 °C ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutagenesis of pLenti-CMV-Neo-PINK1 (C125G)-EYFP or pLVX-puro-OMA1 (E328Q)-EYFP was performed by PCR amplification (CloneAmp HiFi PCR Premix, Takara or Q5 High-Fidelity DNA Polymerase system, NEB) of PINK1 or OMA1 encoding plasmid using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system ...
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bioRxiv - Microbiology 2022Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (NEB) with the primers listed in (Table S3) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The YeastFab assembly is performed according to the reaction system below: 1 μL 10X Buffer for T4 DNA Ligase (NEB), 0.1 μL Purified BSA 100X (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB). Synthetic DNA fragments were ordered from Genewiz and sequences are listed in Supplementary Table 3 ...
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bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
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bioRxiv - Plant Biology 2022Quote: ... a YFP sequence was inserted just after putative endoplasmic reticulum signal peptide sequences in LTPG1 and LTPG2 using the homologous recombination Gibson Assembly system (New England Biolabs), according to Kim et al ...
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bioRxiv - Molecular Biology 2022Quote: ... The yrbA-fs15 DNA templates (with TAG or TGA stop codons) used for in vitro transcription/translation reactions in the classic PURExpress system (New England Biolabs) were prepared by PCR ...
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bioRxiv - Microbiology 2023Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) with the primers listed in Table S4 ...
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bioRxiv - Biochemistry 2023Quote: ... following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) System (NEB # 6901S). The human eIF2A coding sequence was cloned into the C-terminal Mxe GyrA Intein-chitin-binding domains (CBD ...
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bioRxiv - Physiology 2023Quote: Purification of the GS constructs in pMalC6T was performed following the NEB NEBEXpress MBP Fusion and Purification System protocols (Catalog E8201S, NEB). Pellets were thawed in cold water and lysed by sonication for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... The fragments were inserted within the EcoRV site of the Tol1-based transgenesis vector pDon122 (Cosmo Bio, CSR-CT-NU-002-1) using the NEBuilder HiFi DNA Assembly System (New England Biolabs). The Tol1-based transgenic construct (5 ng/µL ...
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bioRxiv - Biochemistry 2022Quote: Drosocin-ribosome complexes were generated by in vitro transcription-translation reactions in PURExpress ΔRF123 in vitro protein synthesis system (New England Biolabs) with the same reaction mix as described earlier in the toeprinting assays ...
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bioRxiv - Molecular Biology 2023Quote: ... and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB). Synthetic DNA fragments were ordered from Genewiz and sequences are listed in Table S6 ...
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bioRxiv - Developmental Biology 2023Quote: Whole 72hpf animals electroporated with Kaede::nls containing constructs for lineage tracing analysis were converted using UV (405 nM) light from either a fluorescent microscope or the OptoWELL system (Opto Biolabs). Kaede conversion took 5-10 seconds per juvenile on the microscope or 1 minute on the OptoWELL for all juveniles at once ...
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bioRxiv - Immunology 2023Quote: ... and subsequently modified by the addition of a 7-methylguanosine cap with the Vaccinia Capping System (New England Biolabs [NEB]) using the NEB Capping protocol (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Amplicons were prepared for sequencing using the Illumina TruSeq system with two rounds of PCR using Q5 Hot Start DNA polymerase (NEB). For the first round of PCR ...
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bioRxiv - Microbiology 2023Quote: ... The mRNA was converted to cDNA using the Invitrogen PhotoScript II First-Strand Synthesis System (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and subsequently modified by the addition of a 7-methylguanosine cap with the Vaccinia Capping System (New England Biolabs [NEB]) using the NEB Capping protocol (NEB ...
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bioRxiv - Cell Biology 2020Quote: cDNA was amplified with primers listed in Primers and sequences section of methods with Q5® High-Fidelity DNA Polymerase PCR System (NEB). PCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR was conducted with the Agilent Mx3005P qPCR System and the Luna® Universal qPCR Master Mix (New England Biolabs). The temperature profile used throughout was the recommended standard for the master mix.
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bioRxiv - Evolutionary Biology 2020Quote: ... The digested DNA fragments were ligated to a barcode adapter-common adapter system (0.045 pmol) with T4 DNA ligase (New England Biolabs, Ipswitch, USA). Each in-line barcode was between four and nine basepairs (bp ...
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bioRxiv - Genetics 2020Quote: ... a plasmid expressing a C-terminal fragment of TDP-43 (aa: 175-414) as GFP fusion was generated here using Q5 site-directed mutagenesis system (NEB, USA) using the plasmid pRS426-GAL1p-TDP43-GFP (Addgene ...
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bioRxiv - Genetics 2020Quote: ... pREP81-FLAG vector and plasmids carrying wild type and mutated forms of rrp1+ and rrp2+ as well as domains of rrp1+ gene used in the yeast two hybrid system were constructed using the Gibson Assembly® Cloning Kit (NEB). All primers used to amplify gene sequences by PCR are listed in Table S3 ...
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bioRxiv - Microbiology 2021Quote: ... and then subcloned into the directly downstream of PreScission protease site pGEX-6P-1 (Cytiva) by Gibson Assembly system (New England Biolabs Inc.) to create pRN108 ...
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bioRxiv - Biophysics 2020Quote: The cell-free transcription/translation mix used in this study is a customized version of the PURE system (40) without ribosomes (PUREexpress Δ ribosomes, New England Biolabs) and the amino acid histidine ...
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bioRxiv - Pathology 2021Quote: ... in an Applied Biosystems StepOnePlus Real-Time PCR System (USA) and tracked with a DNA-intercalant green fluorophore provided with the WarmStart LAMP Kit (NEB, USA). The RT-LAMP amplification was set to 45 minutes for experiments illustrated in Figures 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Toe-printing reactions were carried out in 5-µl aliquots containing a PURExpress transcription-translation coupled system (New England Biolabs, USA) to which the test template was added (16) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the plasmids for the Blasticidin and Zeocin resistance marker-based systems were introduced to T7 Express chemically competent cells (NEB #C2566I) according to the manufacturer’s high-efficiency transformation protocols ...
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bioRxiv - Cell Biology 2022Quote: ... which was incorporated into the TMD by amber stop codon suppression in the PURExpress system lacking all release factors (ΔRF123; New England Biolabs, USA). The release factors RF2 and RF3 ...
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bioRxiv - Cell Biology 2022Quote: ... stop codon and 3’ T7 terminator were used as templates for the coupled in vitro transcription/translation PURExpress system (New England Biolabs, USA). The various SQS constructs used for cysteine crosslinking comprised an N-terminal 3xFLAG tag ...
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bioRxiv - Cancer Biology 2024Quote: 3’-UTRs from mRNAs of interests were cloned using RT/PCR into the pMIR-REPORT™ miRNA Expression Reporter Vector System (Firefly Luciferase) using NEBuilder HiFi DNA Assembly (New England BioLabs). PCR amplification primers are listed in Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: Cleavage of the MBP tag from NlGr7 was completed according to manual instructions of the pMAL protein fusion and purification system (NEB, Inc, USA). The tag (MBP ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were cloned in to the pRF-HU2 vector system 70 using USER enzyme following manufactured protocol (New England Biolabs, MA, USA). Correctly ligated vector was transformed into Agrobacterium tumefaciens strain AGL1 used for V ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and quantified by qPCR on a Bio-Rad CFX Connect Real-Time PCR detection system with the NEBNext Library Quant Kit for Illumina (New England Biolabs, catalog # E7630L). The libraries were normalized to 10 nM ...
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bioRxiv - Biochemistry 2020Quote: ... NEB protocol #M0276 with incubation extended to 1 hour) and a Cap 0 analog (using a Vaccinia capping system, NEB protocol #M2080), following the recommended procedures ...
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bioRxiv - Synthetic Biology 2023Quote: ... approximately1-kb regions upstream and downstream regions of a target gene were amplified by PCR and assembled with BamHI-cut pJQ200sk using the NEBuilder HiFi DNA assembly system (New England Biolabs, Ipswich, MA) to generate a suicide plasmid ...
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bioRxiv - Microbiology 2021Quote: ... promoter replacement constructs were assembled in two stages by attaching flanking regions to a pTef1-HygR-tTef1 resistance marker using a Golden Gate’ (GG) style cloning system (81) established in-house using a pUC19 vector backbone (New England Biolabs, Ipswich, Massachusetts, USA) and kindly provided by Dr ...
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bioRxiv - Plant Biology 2021Quote: ... promoters were inserted into the pH7WG Gateway vector (Department of Plant Systems Biology, Ghent University, Belgium (Karimi et al., 2002)) by the Gibson Assembly kit (New England Biolabs, Ipswich, MA, USA). The tagged PIN7 and PIN4 cDNA entry clones were then recombined with the modified pH7WG destination vector by the Gateway LR reaction (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... and one plasmid was randomly selected from those plasmids and used for expression with a PURE system (PURExpress In Vitro Protein Synthesis Kit, New England BioLabs, Ipswich, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... The gene deletion construct of PnVeA including a phleomycin selectable marker was assembled using Golden Gate cloning system (Engler et al. 2008) using a modified pUC19 (New England Biolabs, Ipswich, Massachusetts, USA) as the backbone vector (John et al ...
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bioRxiv - Microbiology 2021Quote: The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem ...
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bioRxiv - Neuroscience 2020Quote: ... The purified RNA was then reverse-transcribed into cDNA using the Reverse Transcription System (Cat# E6300S, New England BioLabs® Inc., Ipswich, MA, USA). Quantitative RT-PCR analysis of the mRNA level of TNFα (TNFα ...