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Citations for New England Biolabs :
1 - 50 of 197 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... For live cell imaging parasites were stained with Hoechst 33342 (New England Biolabs), transferred to poly-L-lysine-coated μ-slides VI (Ibidi ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling before live-cell imaging with SNAP-Cell 647-SIR (New England Biolabs GmbH) was done according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... An imaging activation mix was prepared by adding RNAse inhibitor (100 μL, New England BioLabs) to 250 μL Imaging Buffer Activator (VIZGEN ...
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bioRxiv - Synthetic Biology 2022Quote: ... We used PURE system (NEB), wheat germ extract (Promega) ...
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bioRxiv - Bioengineering 2024Quote: ... coli Protein Synthesis System (NEB) [48] at 2 x the reaction scale ...
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bioRxiv - Biophysics 2022Quote: Cells used for calcium imaging experiments were labeled using 1 µM SNAP-Surface Alexa Fluor 647 (NEB) and 4 µM Oregon Green 488 BAPTA-1 (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and Gibson system (New England Biolabs) were primarily used ...
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bioRxiv - Biochemistry 2021Quote: ... The HiFi DNA Assembly system (NEB) was used to build the plasmids to express individual ...
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bioRxiv - Biochemistry 2022Quote: ... coli PURExpress®system (NEB E6800S). 1 µL of antibiotic solution was added to 5 µL of PURExpress® reaction mix ...
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bioRxiv - Cell Biology 2020Quote: All the plasmids generated for the opto-release system were cloned using the Gibson Assembly System (NEB) by combining pRK5.1 backbone with desired PCR fragments according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A (E1610S, NEB; 202003, Synaptic Systems), Flag tag Monoclonal Antibody (66008-2-Ig ...
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bioRxiv - Neuroscience 2020Quote: ... or Gibson Assembly system (New England Biolabs). For a list of plasmids and the primers used see Supplemental Table 3 Detailed cloning information will be provided upon request ...
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bioRxiv - Molecular Biology 2021Quote: ... or a complete system (New England Biolabs) (Fig ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by a double digestion system (NEB) and ligation (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... capped with m7G (Vaccinia capping system, NEB), and purified again (RNeasy MinElute columns ...
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bioRxiv - Cell Biology 2022Quote: ... Using the NEBuilder system (New England Biolabs), a DNA fragment encoding GFP or 3×Pk tag with kanR or bsdR was introduced immediately before the stop codon of the rec8 gene cloned in pUC119 ...
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bioRxiv - Microbiology 2023Quote: ... coli Protein Synthesis System (New England Biolabs), then purified with Ni-NTA beads (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... coli protein synthesis system (New England Biolabs) was used for in vitro protein expression ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were labelled with 2.5 μM (0.5 μM for dSTORM imaging) SNAP-Surface Alexa Fluor 546 or 647 (indicated as SNAP546 and SNAP647 in this study) (NEB) for 20 min and optionally counterstained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 37°C for TIR-FM imaging or 1μM permeable SNAP-Cell 647-SiR (New England Biolabs, #S9102S) for 15 minutes followed by a 15 minute wash in cell culture media for confocal imaging ...
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bioRxiv - Molecular Biology 2021Quote: ... These mRNAs were capped (Vaccinia Capping System, NEB) and translated using the nuclease-treated rabbit reticulocyte lysate (RRL ...
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bioRxiv - Genetics 2021Quote: ... in 100 μl system with CutSmart Buffer (NEB), 37°C overnight without shaking ...
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bioRxiv - Molecular Biology 2021Quote: The PURExpress ΔRF123 cell-free translation system (NEB) was used with the following modifications ...
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bioRxiv - Cell Biology 2019Quote: ... and capped using the Vaccinia Capping System (NEB) following the protocols provided by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... using the NEBuilder system (New England Biolabs; NEB). Suitable primers for PCR were calculated by the NEBuilder Assembly Tool 2.0 and are listed in Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... using the NEBuilder system (New England Biolabs; NEB). Suitable primers for PCR were calculated by the NEBuilder Assembly Tool 2.0 and are listed in Table S1 ...
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bioRxiv - Biochemistry 2022Quote: ... and capped with m7G (Vaccinia capping system; NEB). RNAs were visualized by denaturing urea polyacrylamide gel electrophoresis before use to ensure quality ...
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bioRxiv - Cell Biology 2022Quote: ... or the NEBuilder HiFi DNA Assembly system (NEB). All constructs used in this study were confirmed by sequencing ...
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bioRxiv - Microbiology 2023Quote: ... coli protein synthesis system (New England BioLabs inc.). Briefly ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... coli protein synthesis system (PURExpress, New England Biolabs). Reactions were performed according to manufacturer’s instruction and as previously described in 82 with a few modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Transcripts were capped using the vaccinia capping system (NEB) according to the suppliers’ protocol and polyadenylated using the polyA tailing kit (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... RNAs were capped using the Vaccinia capping system (NEB) and poly(A)-tailed at their 3’ ends with poly(A ...
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bioRxiv - Cell Biology 2022Quote: ... and/or Gibson Assembly using the Nebuilder system (NEB). Plasmid backbones were pCFJ1662 and pCFJ910 (Addgene ...
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bioRxiv - Genetics 2019Quote: ... and the Gibson assembly system (E5510S: New England Biolabs). Cas9 and gRNA vectors were transfected into the target cell lines using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the PURExpress translation system (New England Biolabs) reconstituted from purified components ...
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bioRxiv - Molecular Biology 2022Quote: ... The commercially available vaccinia virus capping system (NEB #M2080S) was used as a positive control.
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bioRxiv - Zoology 2022Quote: ... Synthesized RNA was modified by Vaccinia Capping System (NEB), mRNA cap 2’-O-methyltransferase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... by the Vaccinia Capping System (New England BioLabs, M2080S). The RNA was subjected to 3’ adaptor ligation using the same procedure as described above and subsequently enriched with Hydrophilic Streptavidin Magnetic Beads (New England BioLabs ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped using the Vaccinia Capping System (BioLabs) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... we used in vitro PURExpress translation system (New England Biolabs) reconstituted from purified components and supplied with either the WT 70S ribosomes from E ...
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bioRxiv - Biophysics 2020Quote: ... cells using IMPACT™ system protocol (New England Biolabs®) as described before [62] ...
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bioRxiv - Molecular Biology 2020Quote: RNAs were 5’ capped using the Vaccinia capping system (NEB). For each reaction of 20 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAs were capped using the Vaccinia capping system (NEB M2080S) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and capped using Vaccinia Capping System (New England Biolabs, M2080S). This RNA was then introduced to Vero cells using TransIT-mRNA transfection (Mirus Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped using the Vaccinia capping system (NEB M2080S) according to manufacturer’s protocol ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... RNAs were 5′ capped using the Vaccinia Capping System (NEB M2080S) and 3′ biotinylated using the Pierce RNA 3′end biotinylation kit (Thermo Scientific 20160).
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bioRxiv - Molecular Biology 2020Quote: ... Q5 High-Fidelity DNA Polymerase system (New England Biolabs, Ipswich, MA), supplemented with 1 ng/µl carrier herring sperm ...
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bioRxiv - Biochemistry 2020Quote: ... The PURExpress in vitro transcription-translation system was purchased from NEB. GeneJet PCR clean-up kit was purchased from Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Translocation substrates were synthesized in vitro using the PURExpress system (NEB) in the presence of [35S]-methionine for 2 hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and was then installed with 5’cap (Vaccinia Capping System, NEB, USA ...