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Citations for New England Biolabs :
351 - 400 of 9079 citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Synthetic RNA fragments bearing a 3′P were subjected to 5′ phosphorylation with T4 PNK 3′ minus (NEB, cat n° M0236S), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylation was performed using Peptide-N-Glycosidase F (PNGase F) at 37 °C for one hour according to the supplier’s protocol (New England Biolabs, Hitchin, UK). Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 8.0 and adding 2ul/5-10mg dry weight (DW) of PNGaseF (Peptide-N-Glycosidase F) (New England Biolabs, Cat. #P0704S). Samples were incubated with continuous agitation at 150 rpm (Stuart Horizontal Shaker ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylation was performed using Peptide-N-Glycosidase F (PNGase F) at 37 °C for one hour according to the supplier’s protocol (New England Biolabs, Hitchin, UK). Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... a 3′ RNA adapter with a phosphate on its 5’ (5’- /5Phos/r(N:25252525)r(N:25252525)r(N:25252525)rUrGrGrArArUrUrCrUrCrGrGrGrUrGrCrC rArArGrG/3SpC3/-3’) end was ligated to RNA 3’ ends using T4 RNA ligase 1 (NEB, M0437) at 25 °C for 75 min (with gentle agitation every 10 min) ...
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bioRxiv - Bioengineering 2020Quote: To prepare the DNA template for curtain assays λDNA (125 μg, NEB) was incubated with 2 μΜ biotinylated oligo complementary to one of the two 12 nt cohesive ends in λDNA in T4 DNA ligase reaction buffer (NEB ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Molecular Biology 2023Quote: ... 400ng of gDNA was used for T7 endonuclease assay (NEB Cat#M0302). Assays were run in TBE polyacrylamide gels and visualized using Gel Red.
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bioRxiv - Cell Biology 2022Quote: ... Endo H assay was performed following manufacturer’s instructions (New England Biolabs, P0702S). Following addition of Glycoprotein Denaturing Buffer ...
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bioRxiv - Cell Biology 2023Quote: Amylose pulldown assay was performed with Amylose Sepharose beads (New England BioLabs) in a pulldown buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR assays were performed using the Luna Universal qPCR Master Mix (NEB) in a MasterCycler RealPlex 4 (Eppendorf) ...
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bioRxiv - Biochemistry 2020Quote: ... and peptides in one aliquot were deglycosylated by further treatment with 125 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA) for 16 h at 37 °C.
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bioRxiv - Genomics 2020Quote: ... sticky ends were filled by incubating the nuclei for 4h at RT with DNA Polymerase I (New England Biolabs, Cat. N: M0210) and a biotin-14-dATP (Life Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... The cDNA inserts were PCR-amplified using primers specific for either the N- or C-terminal libraries using Phusion DNA polymerase (New England Biolabs, Ipswich, MA). Amplicons were PCR-purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA fragment encoding Sd223-440 was PCR amplified and inserted into a pET28-derived vector providing an N-terminal His-HRV3C-Avi-tag by HiFi DNA Assembly Cloning (New England Biolabs, Ipswich, MA) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified and inserted into a pET28-derived vector providing an N-terminal His-HRV3C-tag by HiFi DNA Assembly Cloning (New England Biolabs, Ipswich, MA) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Microbiology 2020Quote: ... 25 μg of protein was precipitated from cell lysate as described in Methods and treated with peptide-N-glycosidase F (PNGase F; New England Biolabs, MT, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... µM GAT-7N primers (5′- GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L) in the programmable thermal cycler for 11 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... with 1% (w/v) octylglucoside containing 5 U endoglycosidase H (Endo H) or peptide: N-glycosidase F (PNGase F; both NEB, Frankfurt, Germany) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The remaining part of Luc2-ZsGreen (BEI cat# NR-52516) and the amplified N gene were ligated via Gibson assembly (NEB cat# E2611). The coding sequences of generated plasmids were sequence-verified.
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bioRxiv - Molecular Biology 2023Quote: ... This sequence was inserted into a modified pST50 vector56 containing N-terminal Strep-6xHis-TEV-GFP-PreScission tags using Gibson assembly (NEB Cat #: E2611L) to obtain a tagged construct of full-length HURP ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR assays were conducted with Luna Universal qPCR Master Mix (New England Biolabs). Primers for qRT-PCR analyses are listed in Table S4 ...
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bioRxiv - Cell Biology 2020Quote: All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C ...
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bioRxiv - Microbiology 2022Quote: In vitro Cas12a cleavage assays were performed using EnGen Lba Cas12a (NEB #M0653T) and crRNAs that were synthesized commercially (IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads used in this assay were as followed: Streptavidin Magnetic Beads (NEB), EZview(TM ...
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bioRxiv - Cell Biology 2019Quote: Dephosphorylation assays were performed with Calf Intestinal Alkaline Phosphatase (CIP; New England Biolabs). Briefly ...
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bioRxiv - Genetics 2020Quote: ... PCP products were used as template for T7 endonuclease assay (New England BioLabs), following manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The qRT-PCR assay was performed using Luna Universal qPCR Master Mix (NEB) with a total volume of 20 µl ...
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bioRxiv - Genomics 2020Quote: ... DNA was sheared to ∼550 bp fragments using a Covaris S220 sonicator and end-repaired by incubating the samples with T4 DNA Polymerase (New England Biolabs, Cat. N: M0203) for 4h at 20°C ...
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bioRxiv - Cell Biology 2019Quote: ... PACRGΔ1-69 was amplified from the GST-PACRGFL plasmid with PCR primers introduced BamHI (N-terminal) and XhoI (C-terminal) restriction sites for ligation in pGEX-6p1 with the T4 DNA ligase (NEB; Suppl. Figure S9). To generate the PACRG:MEIG1 co-expression vector the same protocol was performed as above using restriction enzyme cloning ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Immunology 2020Quote: ... All in vitro activity assays with recombinant GST-IdeZ or IdeZ (NEB Catalog #P0770S) (1ug per reaction ...
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bioRxiv - Molecular Biology 2022Quote: DNA substrates for gel shift assays were amplified by PCR using Q5 Polymerase (NEB) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Biophysics 2021Quote: ... The reconstitusion was confirmed by MNase assays in which MNases (M0247S, New England BioLabs) digest linker DNA for 30 minutes and the digested products were run on 1% agarose gel ...
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bioRxiv - Immunology 2020Quote: ... The PCR assays using NEB enzyme Q5® Hot Start High-Fidelity (NEB, M0491) with the following thermocycling conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
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bioRxiv - Microbiology 2023Quote: ... LucEV-A71 assay: The plasmid was linearised using Mlu1 restriction enzyme (NEB, Cat # R3198S), and purified using Phenol:Chloroform:Isoamyl Alcohol (PCI 24:25:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and regularly tested for mycoplasma contamination (PCR-based assay from Minerva-Biolabs, Berlin, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Genetics 2021Quote: ... by PCR amplification of target locus followed by T7 Endonuclease I assay (New England Biolabs).
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bioRxiv - Bioengineering 2020Quote: The following components comprised the RT-LAMP assay: 4 mM of MgSO4 (New England Biolabs), 1× final concentration of the isothermal amplification buffer (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... The assay mixture was then analyzed using western blotting by anti-MBP (New England Biolabs) and anti-His (D2951 ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic assays for PNGase F and Endo S were performed according to manufacturer’s instructions (NEB). The effect of the enzymatic assays was either analyzed by Coomassie staining or immunoblotting.
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bioRxiv - Biochemistry 2023Quote: The exonuclease T degradation assays were performed as described by the manufacturer (New England BioLabs). Reactions (20 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... cutting efficiency was determined by T7-endonuclease assay (T7 Endonuclease I, New England Biolabs, M0302) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrations measured using Qubit 1X dsDNA HS Assay) was treated with 5U of RNAse HI (NEB) in RNAse HI buffer for 30 min at 37°C and then nucleic acids were purified with GeneJET Gel Extraction and DNA cleanup micro kit (General cleanup protocol ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...