Labshake search
Citations for New England Biolabs :
51 - 100 of 10000+ citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: The coding sequence of the BTB domain of selected ZBTB family proteins was amplified from cDNA derived from the human HCT116 cell line using Q5 High-Fidelity DNA Polymerase (NEB). Specifically for the Patz1 construct ...
-
bioRxiv - Neuroscience 2019Quote: ... pRSET-dNOS exon 16 DNA was then transformed into T7 Express lysY/lq E.coli protein expression cells (New England Biolabs #C3013) and plated on LB plus ampicillin (60 µl/ml) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... we added 150 – 1000 ng of DNA to 1 μl of prepared MBD2-Fc/protein A beads (New England Biolabs), then washed and eluted the DNA as described by Chiou and Bergey (2018) ...
-
bioRxiv - Neuroscience 2020Quote: ... the enhanced green fluorescent protein sequence and an SV40 polyA) was blunted at both ends using a Klenow fragment of DNA polymerase I (NEB) and cloned into the EcoRV site of the pminiTol2 plasmid (ref) ...
-
bioRxiv - Genomics 2021Quote: ... equal amount of naked DNA and DNA bound by Reb1 protein (~ 5pmol) were incubated with AAG (10 units) and APE1 (1 unit) (New England Biolabs) in a 20 μL reaction containing 1X Thermopol buffer (20mM Tris HCl pH 8.8 ...
-
bioRxiv - Immunology 2020Quote: ... The HDR templates were mixed at fourfold molar excess with a single-strand DNA binding protein (ET SSB, NEB, M2401S) and heated at 95°C for 10 minutes to achieve singlestranded donor oligonucleotides (ssODNs ...
-
bioRxiv - Immunology 2022Quote: Protein expression plasmids were constructed using dsDNA gBlocks from IDT and NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, E5520S) and then transformed in NEB 5-alpha competent cells (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 nM RNAse-free DNA fragments containing gRNA target sequences and 30 nM Cas9 or Cas12a protein (NEB, Ipswich, MA) were mixed in reaction tubes as per manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... DNA templates are prepared by inserting protein-coding sequence of gLuc (pCMV-GLuc control vector, New England BioLabs, Ipswich, MA) into pSP73 vectors (Promega ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fusions to the miniGs-399 protein with C-terminal truncations to RXFP1 were constructed using PCR followed by NEBuilder HiFi DNA Assembly (New England Biolabs). Truncations removed 10 ...
-
bioRxiv - Synthetic Biology 2023Quote: Plasmids encoding RBC biosensor proteins were assembled using standard molecular biology techniques of PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB), restriction enzymes (NEB ...
-
bioRxiv - Plant Biology 2023Quote: ... and AT1G66100 which encode a PR (pathogenesis-related) protein were amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs) from Col-0 genomic DNA with two primer pairs RBCS2B-BglII-PF (5′-CCAGATCTGGAATATTCAATGTTGACTATC-3′ ...
-
bioRxiv - Molecular Biology 2023Quote: ... by replacing the green fluorescent protein (GFP) with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit (New England Biolabs) (Ran et al. ...
-
bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
-
bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
-
bioRxiv - Synthetic Biology 2023Quote: DNA ladder: 1 kb DNA Ladder (NEB) for gel electrophoresis
-
bioRxiv - Genetics 2019Quote: T7 endonuclease assays (M0302L, NEB) were performed following the manufacturer instructions.
-
bioRxiv - Developmental Biology 2020Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
-
bioRxiv - Genetics 2022Quote: ... using a T7E1 assay (NEB), and compared to the activity of Tet2 sgRNA that has been shown to modify the mouse genome efficiently95 ...
-
bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
-
bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
-
bioRxiv - Biochemistry 2022Quote: ... protein was treated overnight with Lambda Protein Phosphatase (NEB #P0753S) at 4 °C.
-
bioRxiv - Biophysics 2020Quote: λ-DNA (NEB) was functionalized at one end by annealing with a biotinylated oligonucleotide/5Phos/AGGTCGCCGCCCTTTTT/Bio (IDT ...
-
bioRxiv - Neuroscience 2021Quote: ... Cas9 protein (NEB) was mixed with all six sgRNAs and injected into embryos at the 1-cell stage ...
-
bioRxiv - Plant Biology 2024Quote: ... SpCas9 protein (NEB), 1 x cut smart buffer (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... 4.0 μg of the DNA were DNA-repaired and DNA-end-repaired using the NEBNext FFPE DNA Repair Mix (New England Biolabs; #M6630) and the NEBNext UltraII End Repair/dA-Tailing Module (New England Biolabs ...
-
bioRxiv - Genomics 2020Quote: ... a dsDNA HS qubit assay and also by a NEBNext library quantitation Assay (New England Biolabs) before sequencing on Illumina’s HiSeq2500v4
-
bioRxiv - Developmental Biology 2021Quote: ... The T7E1 assay (Cat#M0302, NEB) was then applied as in (Sato et al. ...
-
bioRxiv - Cancer Biology 2019Quote: ... formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions ...
-
bioRxiv - Genomics 2020Quote: ... The DNA was repaired using T4 DNA polymerase (NEB) with dNTP (200 μM ...
-
bioRxiv - Microbiology 2021Quote: ... DNAs were ligated using T4 DNA ligase (NEB, USA); competent DH5α (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... NEBuilder HiFi DNA Assembly DNA Master Mix (E2621L, NEB) was used ...
-
bioRxiv - Microbiology 2022Quote: ... DNA ligase and DNA polymerase I (New England Biolabs). The product was used to construct a barcoded cDNA library using the Nextera XT system (Illumina ...
-
bioRxiv - Microbiology 2024Quote: ... DNA fragments were amplified using Phusion DNA polymerase (NEB) in reactions containing 10% DMSO and using GC buffer ...
-
bioRxiv - Microbiology 2022Quote: ... Protein samples were then mixed with Blue Protein Loading Dye (NEB) boiled for 10 minutes and separated on 10% SDS-PAGE gel for 50 minutes at 140 V ...
-
bioRxiv - Plant Biology 2021Quote: ... and extracted nuclear proteins were treated with lambda protein phosphatase (NEB) according to the manufacturer’s instruction.
-
bioRxiv - Genomics 2020Quote: ... Endogenous proteins were captured onto protein G-magnetic beads (NEB; #S1430S), washed extensively in IP buffer and used for POT1wtand POT1V29L source ...
-
bioRxiv - Microbiology 2021Quote: ... Amplification of DNA fragments was performed with either Phusion DNA polymerase or LongAmp DNA polymerase (NEB).
-
bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
-
bioRxiv - Cancer Biology 2021Quote: ... End-repairing of the sheared DNA (using T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB)) was followed by dATP addition (Klenow exo- ...
-
bioRxiv - Molecular Biology 2023Quote: ... using gBlocks synthetic DNA fragments (Integrated DNA Technologies) and NEBuilder HiFi DNA assembly mix (New England Biolabs). HEK293 cells in 12-well plates were transfected with 1.6 μg of CircRNA Mini or mc2 plasmid encoding circMLLT2(3,4 ...
-
bioRxiv - Cancer Biology 2021Quote: ... we used 50 μL Protein A or Protein G Magnetic beads (NEB) and washed twice with PBS with 5 mg/ml BSA and 4 μg of antibody coupled in 500 μl PBS with 5 mg/ml BSA overnight at 4°C ...
-
bioRxiv - Genomics 2020Quote: ΦX 174 virion DNA and Lambda DNA (New England BioLabs) were added to PCR reagents containing 1X Platinum Multiplex PCR Master Mix (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA was end repaired using T4 DNA polymerase (NEB, M0203S), DNA polymerase I large fragment (NEB ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Lane M is DNA marker (NEB 1 Kb DNA Ladder), lane N shows the PCR product from negative control (ddH2O) ...
-
bioRxiv - Genomics 2019Quote: ... the nick-repaired DNA (NEBNext FFPE DNA Repair Mix, NEB) was sheared in a Covaris g-TUBE (Covaris ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was amplified using Phusion DNA polymerase (New England BioLabs) and SARS-CoV-2 specific primers surrounding the open reading frame for the spike protein ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was amplified using Phusion High-Fidelity DNA polymerase (NEB) and MiSeq (300+300 bp PE ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA was purified with Monarch PCR & DNA Cleanup Kit (NEB) and eluted in 20 µL of nuclease-free water ...
-
bioRxiv - Neuroscience 2021Quote: ... elegans genomic DNA using Phusion DNA polymerase (New England Biolabs). nCre expression constructs containing these promoters and the unc-54 3’UTR were created using Gateway cloning (Thermo Fisher Scientific) ...