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Citations for New England Biolabs :
251 - 279 of 279 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Genomic DNA from the spleen was extracted using the Monarch® HMW DNA Extraction Kit for Tissue (NEB #T3060) as per manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using the Monarch® HMW DNA Extraction Kit for Tissue (Cat. No. T3060S, New England BioLabs Inc., MA, USA). DNA from resequencing samples was isolated using either a modified protocol for the DNeasy Blood and Tissue Kit (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs (gDNAs) were extracted using the Monarch HMW DNA extraction kit for Tissue from New England Biolabs (NEB) with the following modifications ...
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bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Genetics 2021Quote: ... high molecular weight genomic (HMW) gDNA was extracted from homozygous 1619P15 pupae using the Monarch HMW DNA extraction kit for tissue (NEB). The Cas9 Sequencing Kit (Oxford Nanopore Technology ...
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bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
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bioRxiv - Neuroscience 2021Quote: ... One piece of mouse frontal cortex tissue (6-10 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Cell Biology 2019Quote: ... Standard qualitative PCRs to check for the general abundance of distinct SPIRE1 splice variants were performed employing cDNAs from mouse brain tissue and Q5 High-Fidelity DNA polymerase (New England Biolabs). Expression vectors encoding mouse SPIRE1 ...
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bioRxiv - Genetics 2020Quote: ... The 5’ and 3’ sequences were amplified from WT adult gDNA extracted using the NucleoSpin Tissue Kit (Machery-Nagel) by PCR using Phusion High Fidelity PCR Master Mix (NEB) and primers 5’-ttttgcggccgcTCTGTTTGAATATGTTTCCGAGAA −3’ and 5’-ttttctcgagTTTCCGCGACAAAAACACAGA-3’ which add restriction enzyme recognition sites NotI and XhoI ...
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bioRxiv - Genetics 2022Quote: ... we also isolated genomic DNA from post-mortem liver tissue using the Monarch® Genomic DNA Purification Kit (NEB, T3010S).
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bioRxiv - Zoology 2022Quote: ... where 250 nanograms of total RNA per tissue was used with the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... Frozen plant tissue was ground to a powder and RNA was extracted using Monarch ® Total RNA Miniprep kits (NEB), RNA integrity and concentration were assessed by agarose gels and a Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: Fifty nanograms of genomic DNA from breast tissue was used to prepare EM-seq libraries as per the manufacturer’s instructions (NEB E7120). The Illumina Novaseq 6000 sequencer was used to sequence the libraries in a paired-end mode ...
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bioRxiv - Systems Biology 2023Quote: ... 25 mg of small RNA from the 12 diverse organs/tissues was resuspended in 10 mL of 1x GlycoBuffer 2 (NEB) and 7.5 mL PNGaseF (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... pSpy0K6 and p7INT.1 was extracted using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs®) according to the manufacturers protocol for Gram-positive bacteria (low input ...
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bioRxiv - Molecular Biology 2024Quote: High molecular weight genomic DNA was extracted from homozygous hDMDTg and hDMDTgSc mice liver using the Monarch HMW DNA Extraction Kit for Tissue (NEB). Oxford Nanopore Technologies Promethion genome sequencing was performed as a service by Novogene ...
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bioRxiv - Cancer Biology 2021Quote: Polyadenylated mRNA was isolated from 1 μg of total RNA from cultured tumor cells or excised tumor tissue using NEBNext® Poly(A) mRNA Magnetic Isolation Module (cat. #E7490, New England Biolabs) and the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Microbiology 2023Quote: High molecular weight DNA was extracted from isolate OI25 and its revertants using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) according to the manufacturer’s protocol with the addition of 50 μg/ml lysozyme (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... The area of the coverslips around the tissue slices was then dabbed dry with a Kimwipe before mounting a SecureSeal hybridization chamber (Grade Biolabs #621502) on top of the tissue slices ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3.3 kb DNA sequence upstream of each respective gene was amplified using PCR and cloned into the vector containing the sequence gfp::rab-7::rab-7 3’UTR amplified from plasmid rgef-1p::gfp::rab-7::rab-7 3’UTR using PCR and primers 5’-atgagtaaaggagaagaacttttca-3’ and 3’-aagcttatcgataccgtcgac-5’ were used to generate tissue-specific gfp::rab-7::rab-7 3’UTR by using Gibson assembly (NEB E2611).
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bioRxiv - Bioengineering 2024Quote: High Molecular Weight (HMW) DNA was obtained using the Monarch® HMW DNA extraction kit for Tissue (T3060L, New England Biolabs), as previously described (Low et al ...
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bioRxiv - Physiology 2020Quote: RNA was extracted from cultured cells or tissue (∼10 mg) stored in RNALater® using Monarch® Total RNA Miniprep Kit (New England BioLabs). For maximal RNA recovery ...
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bioRxiv - Genetics 2019Quote: The 6 cDNA libraries constructed from different tissues of the six perennial species were fragmentased for 20 min using NEB Next dsDNA fragmentase (NEB, Beverly, MA), end repaired and an ‘A’ overhang was added to the ends of the fragments ...
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bioRxiv - Microbiology 2022Quote: ... Template for in vitro transcriptions was amplified from PM-infected tissue cDNA using Phusion polymerase (M0530S/L, New England Biolabs, Ipswich, Massachusetts) and the T7 promoter (5’-TAATACGACTCACTATAGGGGG-3’ ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we isolated DNA from tissue using the Qiagen DNeasy Blood and Tissue kit (Valencia, CA, USA) and created genomic libraries using the NEBNext Ultra II kit (New England BioLabs; Ipswich, MA, USA), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... A portion of leaf tissue (approximately 5 mm2) was homogenized by grinding in 50 μL of 1X Q5 reaction buffer (New England Biolabs Cat. No. B9027S) in a 1.5 mL microcentrifuge tube with a micropestle ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA (tissue samples) or 100ng RNA (cell culture experiments) using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Plant Biology 2021Quote: ... and phloem tissues were also prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs®) ...