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Citations for New England Biolabs :
1 - 50 of 279 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
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bioRxiv - Developmental Biology 2022Quote: ... PTH1R was deglycosylated by 1hour (secondary cell lines) or overnight incubation (primary cells or tissue lysate) with PNGaseF at 37°C (NEB, cat no, P0704S). 1µl of 1M DTT was added to 50µl of cell lysate to ensure complete dissociation of disulphide bonds linking cleaved extracellular domain of PTH1R ...
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bioRxiv - Microbiology 2023Quote: ... lysates (1 mg) were incubated with MNase (10 units/1 mg lysate; NEB #M0247S) at 37°C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... cleared lysate was incubated without or with PNGase F (NEB, 1 μl per 40 μl lysate) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with PNGase F (NEB) following the manufacturer’s instructions or mock treated as a control ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with denaturing buffer (NEB) 95°C for 10 minutes and equal amounts of lysates (180 ug ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with amylose resin (NEB) at 4 °C for 1 h before beads were washed ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were treated with endoglycosidase H (NEB).
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bioRxiv - Plant Biology 2021Quote: ... coli lysates were immobilized on amylose resins (NEB), incubated for 1 h at 4 °C with purified GST-XopS ...
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bioRxiv - Molecular Biology 2020Quote: ... lysate was first treated with DNAse I (NEB) to mitigate genomic DNA viscosity when loading into SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were additionally dephosphorylated using Lambda protein phosphatase (NEB) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then applied to amylose resins (NEB, E8021) and washed with 20 column volumes of binding buffer supplemented with 14 mM beta-mercaptoethanol ...
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bioRxiv - Genetics 2019Quote: ... the nuclear lysates were digested with 800U KpnI (NEB) overnight at 37°C and 950 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... all Orai1 lysate preparations were treated with PNGase (NEB) to remove all glycosylation ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with amylose resin (New England Biolabs) for 2 hours ...
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bioRxiv - Genomics 2023Quote: ... and for tissue (NEB T3060L), following manufacturer’s instructions and introducing few technical improvements ...
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bioRxiv - Biochemistry 2019Quote: ... the cleared lysate was loaded onto a amylose resin (NEB) and eluted with a maltose gradient ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein/lysate sample de-N-glycosylation using PNGase F (NEB), Endo S (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The clarified lysate was added to equilibrated Amylose resin (NEB Cat no ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was added to a chitin resin column (NEB) and washed with Buffer A ...
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bioRxiv - Immunology 2020Quote: ... lysates were digested with endoglycosidase H (endoH) or PNGaseF (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysates were treated with Lambda phosphatase (New England BioLabs). For Western analysis ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was incubated with amylose resin (New England Biolabs), and MBP-Ola1p or MBP-Ola1p-monoGFP was eluted in elution buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with preequilibrated amylose beads (New England BioLabs) for 1 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were loaded onto NEBExpress Ni spin columns (NEB), washed once with lysis buffer containing 10 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... lysates were subject to PNGase F treatment (New England Biolabs) to deglycosylate the transporter proteins ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB8_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB9_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Biophysics 2021Quote: ... The clarified lysate was mixed with amylose resin (New England BioLabs) pre-equilibrated in lysis buffer and incubated for 60 min on a rocking platform ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to amylose resin (New England BioLabs, E8021S). Resin was washed with binding buffer and then eluted with 200 mM maltose in binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were mixed with glycoprotein denaturing buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cleared lysate was applied onto an amylose resin (New England Biolabs), washed with buffer A (25mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified lysate was applied to chitin resin (New England BioLabs) and allowed to bind at 37°C for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysates were incubated with H4K5 Bu antibody (PTM Biolabs) or preimmune IgG per sample and 25 μl of magnetic protein G Dynabeads (catalog no ...
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bioRxiv - Biochemistry 2021Quote: Whole cell lysate or 5 μL of PURExpress (New England BioLabs) reaction was loaded onto a nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysate was added to 10 mL Chitin Resin (NEB S6651S) pre-equilibrated with Tn5 Lysis/Wash buffer and rocked for 1 hour at 4°C ...
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bioRxiv - Immunology 2022Quote: ... lysates were treated with PNGase F or EndoH (New England Biolabs) following manufacturer’s recommendation.
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bioRxiv - Microbiology 2022Quote: ... The lysates were sonicated and denatured with Glycoprotein Denaturing Buffer (NEB) by heating them at 100°C for 10 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified before loading onto the chitin resin (NEB). The sample was treated with 3 U/μL of micrococcal nuclease (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were first incubated with Deglycosylation Mix Buffer 2 (NEB) at 75°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 16.5 µl cleared lysate and uracil DNA glycosylase (UDG; NEB, 1.25 units). Abasic site cleavage was induced by the addition of 100 mM NaOH and incubation at 95° C ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was passed over chitin resin (New England Biolabs, MA) by gravity ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation and then applied to amylose resin (NEB). Resin was washed with 10x column volumes of Column Buffer ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were treated with endoglycosidase H (Endo H) (New England BioLabs) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were mixed with 30 μl of SNAP-magnetic beads (NEB) and incubated for 1 h at 24°C on a thermomixer (900 rpm) ...
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bioRxiv - Cell Biology 2021Quote: ... clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...