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Citations for New England Biolabs :
151 - 200 of 5252 citations for PCR Tubes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The outward PCR experiments were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB). For a 50 µl reaction ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were purified using MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: PCR and plasmid generation – All PCR for plasmid generation was conducted using Phusion polymerase (NEB) using primers listed in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2023Quote: ... PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs, Ipswich, USA), extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Physiology 2024Quote: ... After the PCR 20ul of the PCR product were restriction digested with BamH1 (NEB #xxx) for 1 hr at 37C ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Trimmed gel pieces were transferred to an EP tube and digested with Proteinase K (NEB, P8107) at 37°C for 1 hour ...
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bioRxiv - Genetics 2020Quote: ... One was to use a kit to generate sequencing libraries in one-tube reactions (NEB, E7103S). Another modification was to spike-in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Desired PCR cycle numbers were determined in test endpoint PCRs using Q5 DNA polymerase (NEB M0491L). Final HiC libraries were generated from 4-6 individual PCR reactions ...
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bioRxiv - Genetics 2021Quote: ... and libraries were generated by PCR with NEBNext High Fidelity 2X PCR Master Mix (NEB M0541). Library size was confirmed on a BioAnalyzer prior to sequencing on the NextSeq 550 (40bp read length ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was column-purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs). The IVT reaction including DNAseI digest was performed with the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR MasterMix (New England Biolabs). The PCR products examined with 400-450bp were chosen and mixed in equal density ratios ...
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bioRxiv - Neuroscience 2022Quote: PCR products were extracted with Monarch PCR & DNA Gel Cleanup Kit (New England Biolabs, Ipswich, MA) and sequenced by ABI 3730 automated DNA sequencer.
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bioRxiv - Molecular Biology 2022Quote: ... Site-directed mutagenesis was performed by PCR with NEBNext High-Fidelity 2x PCR master mix (NEB) using overlapping primers with 15 nt overhangs carrying the desired mutation (IDT).
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bioRxiv - Neuroscience 2021Quote: ... Kv1.2wt-T2A and Kir2.1wt-T2A fragments were magnified by PCR (Phusion High-Fidelity PCR Kit, NEB) from pcDNA3-Kv1.2wt (human cDNA template ...
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bioRxiv - Genetics 2022Quote: ... Each PCR consisted of: 20 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 2 µL 10 µM i5 indexing adapter ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and subsequent PCR amplification was done with OneTaq RT-PCR Kit (New England Biolabs). Primers ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were generated from genomic DNA with a Phusion® High-Fidelity PCR kit (NEB) and primers that introduced KpnI and XhoI restriction sites ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed with a Phusion High-Fidelity PCR Kit (New England Biolabs, Ipswich, MA, USA) in 20µL reactions mixed with 2µL cDNA and 10µM primers ...
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bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were mixed with an equal volume of 1× loading buffer (containing SYB green ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using NEBMonarch PCR and DNA Clean-up Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Post-capture PCR was performed with the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) for 14-20 cycles ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using the Monarch® PCR & DNA clean-up kit (New England Biolabs). In vitro expression was performed for 2 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (NEB, T1030) and used as “megaprimers” that are denatured and annealed to the original plasmid (pNG93 ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR reaction was performed using NEBNext High Fidelity PCR Master Mix (NEB, catalog no. M0541L) for 20 cycles ...
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bioRxiv - Biochemistry 2023Quote: PCR mutagenesis was performed by whole plasmid PCR with Q5 high-fidelity polymerase (New England Biolabs) followed by PCR purification (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Each PCR consisted of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer ...
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bioRxiv - Biochemistry 2023Quote: ... RT-PCR reactions were performed using the OneTaq One-Step RT-PCR Kit (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... General PCR for cloning was performed using the NEBNext PCR master mix (NEB cat no. M0541L), with 500 nM of forward and reverse primers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Genetics 2024Quote: ... and libraries were generated by PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541). Prior to sequencing ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were selected by 2% agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix, New England Biolabs), indexing using previously described primers (Buenrostro et al ...
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bioRxiv - Genetics 2021Quote: ... using Q5 polymerase PCR products (NEB). pCMV_AncBE4max (Addgene plasmid #112094 ...
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bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
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bioRxiv - Biochemistry 2019Quote: ... The PCR amplification (Q5 polymerase (NEB)) consisted of 30 cycles (95°C ...