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Citations for New England Biolabs :
1 - 50 of 5252 citations for PCR Tubes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... nuclei were sorted into a tube of a PCR tube strip containing 5 μL 1X CutSmart buffer (NEB, B7204S) per well ...
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bioRxiv - Microbiology 2020Quote: ... Eluted cDNA was transferred to a new PCR tube containing 15 μL of 2X Phusion HF-PCR Master Mix (NEB), 0.5 μL of 30 μM P3/P6 PCR1 oligo mix and 0.5 μl of 15x SYBR Green I (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... The Golden Gate Assembly reaction was set in 200µl PCR tubes (ThermoScientific AB2000) with 2.5 µl 10X T4 DNA ligase buffer (NEB B0202S), 0.5 µl T4 DNA ligase (NEB M0202L) ...
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bioRxiv - Microbiology 2024Quote: ... the DNA in 11.5 µL in a PCR tube was end-repaired by addition of 1.75 µL NEB Ultra II End Repair Buffer (NEB) and 0.75 µL Enzyme Mix followed by thorough mixing via pipetting ...
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bioRxiv - Biophysics 2020Quote: ... The PCR was typically performed in 10 x 50 µL volume by mixing in PCR tubes Phusion HF buffer (1x, New England Biolabs), plasmid template (0.02 ng/µL) ...
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bioRxiv - Microbiology 2020Quote: ... in a new PCR tube 2.5 µL cDNA was combined with 12.5 µL Q5 High-Fidelity 2X Master Mix (NEB, Ipswich, USA), 8.8 µL nuclease free water (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
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bioRxiv - Genomics 2022Quote: ... CRISPEY-BAR barcodes integrated in the genome were amplified with a first step PCR in 8 tubes of 50 uL reactions using Q5 hot-start DNA polymerase (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Microbiology 2019Quote: ... Eluted DNA was transferred to a new tube and PCR enrichment of the adaptor ligated DNA was performed using NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335). The PCR reaction mix was assembled according to manufacturers’ recommendations and placed in a thermal cycler set to the recommended conditions ...
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bioRxiv - Biochemistry 2021Quote: PCR of the tagged DNA was performed in 25 μl reactions in 200 μl thin-walled PCR tubes containing 1X Q5® Buffer (NEB), 1X Q5® GC Enhancer (NEB) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single primer PCR was performed in 2 different tubes for each plasmid using Q5 2X Master Mix (New England Biolabs Cat.No M0492S). After the amplification ...
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bioRxiv - Microbiology 2021Quote: ... PCR with Taq polymerase was performed in a 25-μl volume tube following the manufacture’s guidelines (New England Biolabs, Ipswich, MA, www.neb.com). PCR amplicons were visualized by agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... samples were split into two separate reaction tubes containing 8 μL of PCR product + 1 μL 10X NEB® Buffer #2 (New England Biolabs®). These were incubated in a thermocycler with an initial denaturation of 95 °C for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... 1 µl Exo-CIP Tube A (NEB), 1 µl Exo-CIP Tube B (NEB ...
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bioRxiv - Genomics 2023Quote: ... 1 µl Exo-CIP Tube B (NEB) were mixed and incubated at 37°C for 4 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and then transferred into lysing matrix tubes (Nordic Biolabs) containing 90 mg silica beads (0.1 mm) ...
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bioRxiv - Biophysics 2021Quote: ... in-tube DNAse I (M0303S, New England Biolabs, MA) treatment to eliminate the gDNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... In the same tube added a 1x cut smart buffer (NEB), DNA (100 ng) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were PCR purified using Monarch® PCR DNA Cleanup Kit (New England BioLabs) and eluted with water ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by genotyping PCR using Phusion PCR protocol (NEB) with primer pair of forward ...
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bioRxiv - Genomics 2021Quote: ... Post-PCR each PCR aliquot was exonuclease I (NEB) treated ...
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bioRxiv - Biochemistry 2023Quote: ... and amplified by PCR using Pfusion PCR polymerase (NEB) with specific primers or random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Molecular Biology 2021Quote: PCR was performed with Taq polymerase PCR (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PCR clean-up (Monarch PCR and DNA Cleanup Kit, NEB) and gel extraction (Monarch Gel Extraction Kit ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were purified (Monarch PCR Cleanup Kit (NEB, #T1030S)) and restriction digested with unique 5’ SpeI and 3’ PspXI sites ...
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bioRxiv - Microbiology 2020Quote: ... PCR was done using LongAmp® Taq PCR Kit (NEB) from 40 ng of QMA0248 S ...
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... before PCR using NEB 2xphusion PCR mastermix (NEB, cat #M0531S).
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bioRxiv - Molecular Biology 2019Quote: PCR products were amplified by PCR using the Q5 (NEB®) high-fidelity polymerase following standard conditions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (NEB) for all fragments ...
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bioRxiv - Molecular Biology 2023Quote: All restriction enzymes were purchased from NEB and PCR amplification was performed with Q5 High-Fidelity PCR (NEB) following the manufacturer’s recommendations ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... were PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB) and cloned into pCFD4 (Addgene 49411 ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... in a single tube RNA fragments are sequentially dephosphorylated with T4 PNK (NEB), polyadenylated with E ...
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bioRxiv - Cancer Biology 2023Quote: ... The tubes were then placed on a magnetic stand (New England Biolabs S1515S) to clear and remove the liquid ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Evolutionary Biology 2023Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Immunology 2023Quote: PCR was performed in a 40 μl PCR reaction containing 1x Q5 HotStart Hifi PCR master mix (New England Biolabs), 0.4 μM of Illumina adapter PCR primers (ILM_p5_PCR ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... twelve-cycle PCR reactions (Phusion High Fidelity PCR kit, New England Biolabs), using primers that added Flow Cell binding sequences ...
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bioRxiv - Biochemistry 2022Quote: ... Following which the PCR products were purified by PCR cleanup kit (NEB) and a second PCR was done with primers containing the READ1 ...
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bioRxiv - Immunology 2022Quote: ... a quantitative PCR reaction (10 µl 2 x PCR master mix (NEB), 1 x SYBR green (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were purified with Monarch PCR & DNA Cleanup Kit (NEB, T1030L). For the hHBB-Fluc control mRNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were generated using the 2x Q5 PCR Master Mix (NEB) in accordance with manufacturer instructions ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Resulting PCR product was purified using Monarch PCR purification kit (NEB, USA) and were cloned into pCS2+ vector using restriction enzymes BamH1 and SnaB1 based cloning to generate pCS2-Satb2 ...