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Citations for New England Biolabs :
351 - 400 of 1083 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
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bioRxiv - Molecular Biology 2023Quote: ... After PAGE purification the RNA was treated with polynucleotide kinase (NEB) to remove the residual 2’ ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow fragment (5 U) & T4 polynucleotide kinase (15 U) (NEB; M0201). The A-tailing was performed with Klenow exo-fragment (15 U ...
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bioRxiv - Biochemistry 2023Quote: Kinases were treated with 0.5 µL lambda phosphatase (New England Biolabs) per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... 25nM of DNA was radiolabeled using T4-Polynucleotide kinase (NEB M0201S) and 0.5μl of ATP(γ-32P ...
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bioRxiv - Microbiology 2023Quote: ... Here amplicons were incubated with T4 polynucleotide kinase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNAs were dephospholylated by T4 polynucleotide kinase (New England Biolabs) and ligated with linker for reverse transcription ...
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bioRxiv - Molecular Biology 2023Quote: The kinase domain variant library was digested with PstI-HF (NEB) and NdeI-HF (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... The purified DNA was 5’ phosphorylated with T4 Polynucleotide Kinase (NEB) and ligated with homemade T4 DNA ligase ...
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bioRxiv - Molecular Biology 2023Quote: ... and 25U T4 polynucleotide kinase (10 U/μL) (New England Biolabs). The radiolabelled RNA was purified by PAGE and resuspended in 40 μL nuclease-free water ...
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bioRxiv - Biophysics 2024Quote: ... Inserts were phosphorylated by incubation with T4 polynucleotide kinase (NEB, #M0201) in T4 DNA ligase buffer (# ...
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Bioengineering 2023Quote: Right side probes were 5’ phosphorated with T4 Polynucleotide Kinase (NEB). Probes were then column cleaned with ssDNA/RNA Clean & Concentrator (Zymo D7010 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 units of T4 Polynucleotide kinase (M0201L, New England Biolabs), in a total volume of 50 uL ...
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bioRxiv - Immunology 2023Quote: ... End dephosphorylation was performed using T4 polynucleotide kinase (PNK) (NEB M0201) for 1 hour at 37°C and ligation was performed using the T4 RNA ligase I (NEB M0204 ...
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bioRxiv - Genomics 2023Quote: ... We annealed oligos in the presence of T4 Polynucleotide Kinase (NEB). We then performed Golden Gate assembly with diluted oligos ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM DNA oligo in T4 polynucleotide kinase buffer (NEB). This mixture was then incubated at 37°C for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... and hydroxyl repair was performed using T4 Polynucleotide Kinase (PNK; NEB). Purified RNAs were subsequently ligated with the 5’ RNA adaptor (RA5 ...
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bioRxiv - Cell Biology 2024Quote: ... were combined with protein kinase buffer (PK buffer, New England Biolabs: 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biophysics 2022Quote: ... The DNA fragment was amplified by PCR by inserting a C-terminal 6x His tag and cloned into the pETDUET-1 vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... A 25 μg aliquot of CP-treated CTDNA was diluted to 100 μL of 1X nucleoside digestion mix reaction buffer (NEB, Ipswich, MA) at pH 5.4 and treated with 2.5 μL NEB nucleoside digestion mix (1 μL or 1 U per 10 μg CTDNA ...
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bioRxiv - Molecular Biology 2024Quote: Genomic phage DNA was digested using either a combined treatment of DNaseI and ExoI to create single nucleotides or a commercially available nucleoside digestion mix (NEB, Ipswich, USA).
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bioRxiv - Genetics 2021Quote: ... The forward primer was phosphorylated with T4 polynucleotide kinase (New England Biolabs) and used in a high fidelity PCR reaction (see below ...
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bioRxiv - Genetics 2020Quote: ... 0.75 M NaCl) and 2 μl protein kinase K (NEB, Ipswich, USA) during 2 h at 60 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosome protected RNA fragments were 3’ dephosphorylated with T4 polynucleotide kinase (NEB) in 150 mM MES-NaOH pH 5.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were monophosphorylated on their 5’ ends using T4 polynucleotide kinase (NEB) and either 3 mM unlabeled ATP (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Obtained RNAs were 5’-radiolabelled with T4 polynucleotide kinase (New England Biolabs) and [γ32P] adenosine triphosphate (ATP ...
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bioRxiv - Molecular Biology 2020Quote: ... 3’ end dephosphorylation was performed with T4 polynucleotide kinase (New England Biolabs) before addition of a pre-adenylated linker using RNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... the CRISPR pAS guide oligonucleotides were phosphorylated (T4 poly nucleotide kinase; NEB), annealed and ligated (Quick Ligase kit ...
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bioRxiv - Molecular Biology 2020Quote: ... phosphorylated by T4 polynucleotide kinase at the 5’ ends (NEB, Ipswich, MA) at 37°C for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... End-labelling was done using γ32-ATP and T4 polynucleotide kinase (NEB). Radiolabelled fragments were incubated with purified proteins in buffer containing 40 mM Tris acetate pH 7.9 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the 5′-hydroxyl group was repaired with T4 polynucleotide kinase (BioLabs). The libraries were generated using TruSeq small-RNA adaptors and sequenced using NextSeq500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μl of 10 U/μl T4 polynucleotide kinase (NEB; Cat#: M0201L), 1 μl of 5 U/μl Klenow DNA polymerase ...
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bioRxiv - Synthetic Biology 2021Quote: ... the forward primers were phosphorylated using T4 polynucleotide kinase (New England Biolabs) to facilitate T4 ligation later ...
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bioRxiv - Neuroscience 2020Quote: ... a catalytic subunit of protein kinase A (PKA Cα, New England Biolabs); Ca2+/calmodulin-dependent protein kinase II (CaMKII ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 units of T4 polynucleotide kinase (PNK) (New England Biolabs, #M0201S) in PNK buffer ...
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bioRxiv - Microbiology 2021Quote: ... Fragmented RNA was treated with 10 U of T4 polynucleotide kinase (NEB) in 20 μl reactions containing 1 mM ATP ...
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bioRxiv - Microbiology 2021Quote: ... The two oligos were then phosphorylated using T4 polynucleotide kinase (NEB, USA) at 37°C for 1 hr ...
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bioRxiv - Bioengineering 2020Quote: ... Probes were labeled with ATP-P32 using T4 polynucleotide kinase (NEB®) and blot was exposed to a phosphor screen (GE® ...
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bioRxiv - Genetics 2022Quote: ... The three regional oligo pools were phosphorylated using T4 Polynucleotide Kinase (NEB) as recommended by the provider using 300 pmoles of each oligo pool and incubation for one hour at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and phosphorylated at the 5’ termini by T4 Polynucleotide Kinase (NEB, M0201L). The pBluescript plasmid was cut by EcoRV and dephosphorylated by Calf Intestinal Alkaline Phosphatase (CIP ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons were incubated with T4 polynucleotide kinase (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Guides were inserted into vectors using T4 polynucleotide kinase (New England Biolabs), and the final combined guide RNA vector was assembled by Golden Gate Assembly using Quick Ligase (New England Biolabs) ...
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bioRxiv - Biophysics 2021Quote: The NNS primers were phosphorylated with T4 polynucleotide kinase (NEB, cat#M0201S). 20 μL phosphorylations was prepared according to the following recipe ...
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bioRxiv - Genomics 2021Quote: DNA end modification: 10 U T4 Polynucleotide Kinase (New England Biolabs, M0201) in 60 μl 1×T4 DNA Ligase reaction buffer were added to each sample and incubated at 37°C for 30 minutes.
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bioRxiv - Cell Biology 2021Quote: ... A positive control was prepared by substituting casein kinase II(NEB, P6010S) for galectin 3 in the reaction mixture.
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bioRxiv - Genomics 2021Quote: ... The 3’ ends were dephosphorylated with T4 polynucleotide kinase (T4 PNK; NEB) in the following reaction mix ...