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Citations for New England Biolabs :
401 - 450 of 598 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... plasmid was made by inserting the TauRD sequence (Tau amino acids 244-371) in pHUE82 by Gibson assembly using the Gibson Assembly Master Mix (New England Biolabs). Plasmid pHUE-TauRD (C291A/P301L/C322A/V337M ...
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bioRxiv - Cell Biology 2020Quote: ... A fragment of human Plexin-B1 cDNA (encoding amino acids 1-535) was cloned into the pcDNA5 vector using HindIII (R0104S, NEB) and XhoI (R0146S ...
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bioRxiv - Cell Biology 2021Quote: ... Single or multiple amino acid mutants of TULP3 and ARL13B were generated by Q5 site directed mutagenesis (New England Biolabs). For biotinylation experiments ...
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bioRxiv - Cell Biology 2019Quote: ... the cDNA of RDGB corresponding to amino acids 947-1259 was subcloned in pJFRC::GFP vector using the restriction enzymes BglII and NotI (NEB). A flexible linker of Gly(G)-Ser(S ...
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bioRxiv - Microbiology 2023Quote: ... A stop codon was inserted after amino acid residue 370 by site directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB), in order to express the truncated N1-370 construct ...
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bioRxiv - Biochemistry 2023Quote: ... serine or aspartic acid were generated by engineering the corresponding constructs using Q5 site-directed mutagenesis (New England Biolabs, E0554). For this purpose ...
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bioRxiv - Biophysics 2023Quote: ... and 575-585 for ΔX-Y contact) were replaced with a 12 amino acid GSSG linker using the KLD enzyme mix (NEB). Expression and purification were carried out the same as for the wildtype protein ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into murine Shh between amino acids 92N and 93T (corresponding to N91 and T92 in human Shh) by using Gibson assembly (HiFi Assembly Kit, NEB). Where indicated ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Microbiology 2024Quote: Amino acids substitutions in ompT were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... site-directed mutagenesis of the codon encoding lysine at amino acid position 41 was altered to alanine using the Q5 site-directed mutagenesis kit (New England Biolabs) using pPM11 as a template ...
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bioRxiv - Molecular Biology 2021Quote: ... Mono and di-nucleosome DNA bands were excised from the gel and purified by β-agarase (NEB) digestion followed by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Bioengineering 2021Quote: ... the PCR reaction was purified via Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). Plasmids were constructed via the Hot Fusion Assembly Method (Fu et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Gel-extracted PCR products were assembled in a cut-ligation reaction via BsaI-HFv2 (New England Biolabs) into vector pRRES208.482 (kindly provided by Alison Huttly ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded directly onto the gel along with a pre-stained protein marker (New England Biolabs), and were run with a constant voltage of 60 V ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR fragments were gel purified and equal amounts of DNA were subject to DrdI (New England Biolabs) enzyme digest following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of gel purified DNA was used as a template for in vitro transcription (NEB E2040S) carried out according to the manufacturer’s instructions with the addition of 0.1µl of Cy3 (Sigma PA53026 ...
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bioRxiv - Genomics 2019Quote: Size selection using PAGE gels was recommended by three of the manufacturers (Illumina, NEXTflex, and NEB kits) and was performed for these kits for better comparisons ...
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bioRxiv - Genomics 2021Quote: ... 10 ng A-tailed gel-isolated PCR product and 1 µl T4 DNA ligase (New England Biolabs) were mixed in a total reaction volume of 10 µl and incubated for 15 min at room temperature ...
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bioRxiv - Physiology 2019Quote: ... PCR products from the mutant allele of the F1 heterozygous were purified from gel bands (NEB #T1020S) and analyzed by Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... These synthesized gBlocks were cloned into gel-purified restriction enzyme digested backbone by the Gibson assembly (NEB). For anti-RBD antibody clones’ light chain plasmid cloning ...
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bioRxiv - Biochemistry 2022Quote: ... products corresponding to the correct size were excised and purified using Monarch DNA Gel Extraction kit (NEB). For the second round of PCR ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products corresponding to the correct size were excised and purified using DNA Gel Extraction kit (NEB). 100 ng of each gel-purified PCR products (total of 19 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then loaded onto an 8% polyacrylamide gel alongside a 50 bp ladder (New England Biolabs) and resolved at 100 V for 45 minutes in TBE buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... A gel lambda ladder PFG marker and yeast chromosome PFG marker (both from New England BioLabs, Inc.) were cast next to the gel plugs ...
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions were run on 1.2% TAE agarose gel with a standard 1kb ladder (New England BioLabs) to confirm formation of products ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Neuroscience 2020Quote: ... amino acid sequence identical to GenBank: BC002453.2) was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA) and inserted into the TOPO cloning site in pET151/D-TOPO E ...
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bioRxiv - Molecular Biology 2021Quote: ... or more amino acid substitutions in SecPH with Q5 PCR methodology (Q5®Site-Directed Mutagenesis Kit, New England Biolabs, E0554) or PCR site-directed mutagenesis strategy ...
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bioRxiv - Immunology 2021Quote: ... were generated by introducing the corresponding amino acid mutations (Extended Data Fig. 5) using the Q5® Site-Directed Mutagenesis Kit (NEB) and per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA coding region corresponding to RDGB(USR1-LNS2)Δ (amino acids 1-472) was subcloned into pUAST-attB by using the restriction enzymes NotI and XbaI (NEB). Similarly ...
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bioRxiv - Cell Biology 2023Quote: ... The C-terminal truncation of murine STING (to include only amino acids 1-339) was accomplished using NEB Q5 High-Fidelity 2X Master Mix (NEB M0492S) per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). pEGFP-N1 and pEYFP-N1 were obtained from Clonetech (Mountainview ...
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bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Amino acid sequencing and glycan localization were carried out by digesting the antibody samples with trypsin or chymotrypsin (New England Biolabs, Ipswich, MA) followed by LC-MS/MS analysis of proteolytic fragments with an Orbitrap Fusion (Thermo ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Products from the first round PCR were then purified using Monarch DNA Gel Extraction Kit (New England Biolabs) and used as the templates for the second round insert PCR ...
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bioRxiv - Bioengineering 2022Quote: One μL of amplicons from colony PCR is mixed with 1 μL of Gel Loading Dye (6x; NEB) and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500 ng of gel purified mononucleosomal DNA were repaired using the PreCR Repair Mix Kit (New England Biolabs) with 100 µM dNTPs in a 50 µL reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... Gel-purified PCR product was then cloned into the pGL4.23 vector using T4 DNA ligase (New England Biolabs). Finally ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... as was the pCDH vector before purification again with Monarch® DNA Gel Extraction Kit (New England Biolabs). The ACE2 fragment was ligated with pCDH vector using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... PCR products were separated on agarose gels and purified for Sanger Sequencing (Microsynth) using ExoSAP-IT reagent (NEB). Chimeric PCR products were subcloned before sequencing using StrataClone PCR cloning kits (Agilent).
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bioRxiv - Biochemistry 2021Quote: ... The reactions were then stopped by 2 μl of no SDS-purple gel loading dye (New England BioLabs) and separated on 0.6% agarose gel at 100V for 50 min in 1XTBE ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each reaction was visualised on a 1 % agarose gel using a 1 kb DNA ladder (New England Biolabs) and a Lambda ladder (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The two PCR fragments were each gel-purified and assembled together using a 2x Gibson master mix (NEB). Gibson assembly was possible due to a 23 bp sequence shared between the two PCR fragments ...
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bioRxiv - Microbiology 2022Quote: ... We noted an improvement of the Gibson assembly efficiency when gel-purifying DNA using the Monarch kit (NEB) compared to other kits ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of each reaction was combined with 2 µL of 6X Purple Gel Loading Dye (NEB B7024S) and 11 µL H2O and run on a 1.2% agarose gel containing 1X GelGreen Nucleic Acid Stain (Biotium 41005 ...
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bioRxiv - Biochemistry 2022Quote: Ligation workups from the previous step were supplemented with 3 μL of 6X purple gel loading dye (NEB), heat denatured at 85 °C for 1 min ...
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bioRxiv - Genomics 2022Quote: ... The correct size band was cut and purified with the Monarch Gel Extraction Kit (New England BioLabs T1020L). The insert single-stranded DNA was diluted to 1 μM with H2O ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 uL of PCR products were mixed with 5.5 uL ddH2O and 1.9 uL 6X gel loading dye (NEB) and loaded onto 1% w/v agarose gels cast with SYBR Safe DNA gel stain ...