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Citations for New England Biolabs :
251 - 300 of 598 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... purified using a Monarch gel extraction kit (New England BioLabs T1020L), and sequenced by a commercial company (ACGT ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then combined with 6x gel loading dye (NEB) and ran on a 1% ...
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bioRxiv - Neuroscience 2021Quote: ... Gels were then placed in Proteinase K (New England Biolabs: P8107S) diluted 1:100 (8 U/mL ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were recovered by gel purification columns (New England Biolabs), and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... All fragments were gel purified and ligated using Gibson assembly (NEB), then transformed into stable competent E ...
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bioRxiv - Molecular Biology 2023Quote: ... 6× NEB™ Purple Gel Loading Dye (no SDS) (NEB, B7025S) was added to samples before separation on a 0.8% agarose/TAE gel in 1xTAE at 30V for 22 hours at 4°C.
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bioRxiv - Microbiology 2023Quote: ... DNA fragments were recovered by gel purification columns (New England Biolabs), and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Six microliters of 6x Gel Loading Dye (B7025S, New England Biolabs) and 1.5 μL of 2.5 mg/ml EtBr were added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Six microliters of 6x gel loading dye (B7025S, New England Biolabs) and 1.5 μl of 2.5 mg/ml EtBr were added to each RNA sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μl of 6x Gel Loading Dye (B7025S, New England Biolabs) and 1 μl of 2.5 mg/ml EtBr were added to the RNA size markers.
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bioRxiv - Molecular Biology 2023Quote: ... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... separated by the SIM sequence (amino acids 469-478) going outwards using Q5 polymerase (NEB). PCR product was purified ...
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bioRxiv - Developmental Biology 2020Quote: ... and 30 μg was diluted 1:3 in gel loading buffer (NEB), sonicated ...
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bioRxiv - Microbiology 2019Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during extension overlay PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were loaded with 6X purple gel loading dye (B7025, NEB) and electrophoresed alongside a low molecular weight ladder (range 25 to 766 base pairs ...
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bioRxiv - Microbiology 2020Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during PCR ...
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bioRxiv - Genetics 2022Quote: ... Amplified and gel-purified PCR ORFs were XbaI (R0145S, NEB, Ipswich, Massachusetts) and PacI (R0547S ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 µl ddH2O ...
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bioRxiv - Plant Biology 2019Quote: ... Following agarose gel (cut with XbaI/PstI) (New England Biolabs, Ipswich MA). Gel extractions were performed using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... using gel-extracted DNA as template and Taq polymerase (New England BioLabs). The probe product was cleaned using mini Quick spin columns (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA). In vitro transcription of this template was performed using the mMESSAGE mMACHINE T7 kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... and 1x SDS Gel Loading Dye (New England BioLabs, cat. no. B7024S) – this treatment displaced MDA5 and LGP2 but not streptavidin from biotinylated RNA ...
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bioRxiv - Biophysics 2022Quote: ... and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
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bioRxiv - Microbiology 2023Quote: ... in the presence of 2,000 gel units micrococcal nuclease (New England Biolabs) for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reactions were mixed with Gel Loading Dye Blue (New England BioLabs Inc.) and ran on a 2% agarose gel with 1X SYBR Safe DNA gel stain (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The gel was then digested with proteinase K (NEB, cat. no. P8107S) at a final concentration of 8 U/ml in digestion buffer (50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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bioRxiv - Molecular Biology 2019Quote: ... and decapped using Tobacco Acid Pyrophosphatase (TAP) enzyme (Epicentre, can be replaced by RppH from NEB) and purified by phenol/chloroform extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... Customized PURExpress reconstituted systems lacking all amino acids and release factors (Δaa, ΔRF123) (New England Biolabs) (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic deoxyribonucleic acid (gDNA) was extracted using Monarch Genomic DNA Purification Kit (New England BioLabs, T3010S) following procedures for Tissue gDNA isolation ...
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bioRxiv - Genomics 2019Quote: ... the RNA was gel extracted and was dephosphorylated using polynucleotide kinase (NEB #M0201S). A Universal miRNA cloning linker (NEB # S1315S ...
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bioRxiv - Immunology 2019Quote: ... Gel filtered proteins were labeled with either SNAP-Cell 505 (NEB, catalog S9103S) or SNAP-Cell TMR (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... PCR reactions were supplemented with Gel Loading Dye Purple (6x) (New England Biolabs) and separated on a 2.5 % low melt agarose (BioRad ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch DNA Gel Extraction Kit (NEB, T1020S) and subjected to sequencing.
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bioRxiv - Genomics 2021Quote: ... polony gels were digested with 800 unit/mL (final concentration) TaqI (NEB R0149) diluted in 1× CutSmart buffer at 60°C for 1 hour.
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bioRxiv - Microbiology 2021Quote: ... cells were lysed in 150μL of 1x Gel Loading Buffer (New England Biolabs), sonicated and boiled 5min at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... into gel purified pFA6a-TRP1-GAL1 digested with PacI/AscI (New England BioLabs). Successful subcloning was confirmed by sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... agarose gel and PCR clean ups were performed using Monarch DNA kits (NEB). Plasmid DNA constructs were confirmed by sequencing on the ABI 3130xl capillary machine (Applied Biosystems ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... cells were lysed in 150μL of 1x Gel Loading Buffer (New England Biolabs), sonicated and boiled 5min at 95°C ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... PCR fragments were purified using Monarch DNA Gel Extraction Kit (New England Biolabs), and were digested with EcoRI and BamHI (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Products were purified using the Monarch DNA gel purification kit (New England Biolabs), and Sanger sequenced.
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bioRxiv - Biophysics 2021Quote: ... The reactions were quenched using 1x purple gel loading dye (New England Biolabs). The reaction products were heated at 95 °C/5 min and separated on 1% agarose gel in Tris/Acetic acid/EDTA (TAE ...
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bioRxiv - Genetics 2022Quote: ... Each of these fragments were extracted using a DNA Gel Extraction Kit (NEB), quantified ...
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bioRxiv - Bioengineering 2022Quote: ... PCR fragments were then purified using a gel extraction kit (New England Biolabs) and subjected to sanger sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... Gel-purified PCR products were A-tailed using Taq polymerase (New England Biolabs) for 30 min at 72 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1800 bp was excised and processed using Monarch DNA Gel Extraction kit (NEB). Purification kits were used according to manufacturer’s instructions ...