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Citations for New England Biolabs :
51 - 100 of 9480 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Ig kappa or Ig lambda constant domains using the Quick Ligase cloning system (New England BioLabs) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... The SH3 domain DNA sequence of ABP1 used in the DMS experiments was cloned into pUC19 (NEB) via restriction enzyme cloning with 78bp homology arms surrounding the SH3 DNA (from genomic DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... Domain dissection mutants were subcloned from sfGFP-TAOK2 using restriction enzymes HindIII and MfeI (New England Biolabs). All resultant plasmids were verified by sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... The 3-4FnIII fragment and randomized 5FnIII domain were digested with BsmBI-v2 (New England Biolabs R0739S), purified by PCR cleanup kit (Fisher Scientific FERK0702) ...
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bioRxiv - Neuroscience 2022Quote: ... Domain deletion mutants were subcloned from sfGFP-TAOK1 using restriction enzymes EcoRI and MfeI (New England Biolabs) and assembled using NEBuilder HiFi DNA Assembly-New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The destabilization domain was removed from the plasmid via restriction enzymes BamHI and EcoRI (New England Biolabs) for constitutive expression in the YUMM1.7 cell lines.
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bioRxiv - Biophysics 2023Quote: ... and the DNA of the SL5-6 domains was then digested using BsaI (New England Biolabs #R0535) followed by Mung Bean Nuclease (New England Biolabs #M0250) ...
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bioRxiv - Neuroscience 2023Quote: ... A synthetic DNA block (IDT) coding for the scrambled E domain was subcloned using Gibson-cloning (NEB). GST-α-syn 96-140 Scr and GST-α-syn 96-110 Scr plasmids were synthesized by GenScript (Piscataway ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
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bioRxiv - Neuroscience 2022Quote: ... Receptor cDNAs were amplified by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs) from cDNA of mixed-stage populations of wild-type C ...
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bioRxiv - Immunology 2023Quote: ... and L234A/L235A/P329G (LALAPG) mutated CH2 domains were synthesized and purchased from IDT with BamHI/BsrGI (NEB) restriction enzyme sites at the ends ...
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bioRxiv - Biochemistry 2023Quote: ... MEMO1 was expressed as fusion with the chitin-binding domain and intein using vector pTYB12 (New England BioLabs) in E ...
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bioRxiv - Cell Biology 2021Quote: ... Receptors were surface labelled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... where the Emp24 transmembrane domain (residues 173-193) was replaced by 26 leucines using Gibson assembly (New England Biolabs). To construct pSP-FLAG-Cp ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA sequence pertaining to Mxe intein-chitin-binding domain (CBD) was PCR amplified from pTXB1 (New England Biolabs) and ligated in frame at the C-terminus of H2AX(1-134) ...
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bioRxiv - Cancer Biology 2023Quote: ... To produce the ABI1 Isoform 2 deleted SH3 domain we performed Q5 site-directed mutagenesis (New England Biolabs Inc.) with forward primer 5’-TAGCTCGAGGTTAACGAATTC - 3’ and reverse primer 5’-TTTCTCAATATAATTCTTGGGG - 3’ synthesized by IDT and then verified the sequence (Genewiz) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Genetics 2023Quote: ... along with a repair template containing the desired mutation with the Gibson Assembly kit (NEB). The Nf1 donor construct contained the following elements ...
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bioRxiv - Synthetic Biology 2021Quote: ... An equimolar mix of all domain fragments was prepared for ligation into the digested CAR chassis vector with T4 ligase (NEB). The ligated plasmids were transformed and amplified in chemically-competent E ...
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bioRxiv - Molecular Biology 2019Quote: ... The gene fragment covering the predicted middle domain (residues 807–1097) was amplified by PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs), using pcDNA3-Xpress-G5 as template (Francisco-Velilla et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the editing domain-coding segment of leuS gene was PCR amplified by using Phusion® High-Fidelity DNA Polymerase (NEB), primers 1 and 2 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of cDNA was amplified by PCR with primers that amplify about 300-600 bp surrounding the sites of interest (outside the length of the antisense domain) using OneTaq PCR Mix (NEB). The numbers of cycles were tested to ensure that they fell within the linear phase of amplification ...
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bioRxiv - Biochemistry 2021Quote: pMAL-c4E vectors carrying in-frame fusions of the EFR cytoplasmic domain with the N-terminal maltose-binding protein (MBP) tag were transformed into Rosetta 2 cells (NEB) for recombinant protein expression ...
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bioRxiv - Biochemistry 2020Quote: ... Synthesized first-strand complimentary DNA was used to amplify the heavy-chain variable domains using Q5 high-fidelity DNA polymerase (New England Biolabs) and the described primers (CALL001 ...
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bioRxiv - Cell Biology 2021Quote: ... GST aPKC PBM and his aPKC kinase domain-PBM (residues 259-606) were cloned as previously described (10) using Gibson cloning (New England BioLabs), Q5 mutagenesis (New England BioLabs ...
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Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylationbioRxiv - Biophysics 2019Quote: ... All constructs with large domain insertions and deletions were made using standard protocols for Gibson assembly (New England Biolabs, MA). All point mutants used were generated using the standard Quikchange protocols (Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... using a plasmid that codes for the desired protein sequence fused to an N-terminal intein and chitin binding domain (CBD) as part of the IMPACT purification system (NEB). 2 liters of cells were grown to an OD600 of 0.6 and induced with 1 mM IPTG for 3 hours at 25 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain variable domain was then amplified from the cDNA using Q5 high-fidelity DNA polymerase (New England Biolabs) with the described primers (CALL001 ...
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bioRxiv - Cancer Biology 2022Quote: Codon-optimized sequences encoding the DNA-binding domains of human BATF (AA 28-87) and JUNB (AA 269-329) were cloned into pMAL-C2X (NEB). The sequence encoding human IRF4 DBD (AA 19-120 ...
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bioRxiv - Biophysics 2023Quote: ... All constructs with large domain insertions and deletions were made using standard protocols for Gibson assembly (New England Biolabs, MA). All point mutants used were generated using the standard Quikchange protocols (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Immunology 2020Quote: ... Single antigen specific memory B cells were sorted on BD FACS Aria II into 96-well PCR plates (Axygen) containing 10 μl per well of lysis buffer (10 mM DPBS, 4 U Mouse RNase Inhibitor, NEB). Plates were immediately frozen on dry ice and stored at 80 C or processed for cDNA synthesis.
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bioRxiv - Genetics 2021Quote: ... the sequences encoding both the PR/SET domains and full-length PRDM9 were transferred into the pMAL-c6T (New England Biolabs #N0378) vector ...
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bioRxiv - Microbiology 2019Quote: ... All constructs were verified by sequencing andSsite directed mutagenesis for the generation of point mutations as well as domain deletions were performed using Quick Change Site-Directed Mutagenesis protocol using Phusion polymerase (New England BioLabs, USA). Mutations/deletions introduced were verified by DNA sequencing.
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bioRxiv - Immunology 2020Quote: Diabodies were produced from scFv clones by PCR of the VL and VH domains using Q5 DNA polymerase (NEB, Cat: M0491S) to shorten the amino acid linker between the domains to SSGGGGS ...
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bioRxiv - Cancer Biology 2023Quote: ... CRISPR sgRNA resistant synonymous or functional domain point mutations were introduced by PCR mutagenesis using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2631). Stable cell lines were generated using the pLU vectors and selected with puromycin or blasticidin ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: Purified DNA was amplified with human-or mouse-specific primers covering the RBM20 RS-domain mutation hotspot with Nextera-compatible adapters (Sup. Table 1) using Q5®Hot Start High-Fidelity 2X Master Mix (NEB). One microliter of a 1:100 dilution was used for a second PCR attaching sample-specific index barcodes (Nextera XT Index Kit v2 Set A ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Biochemistry 2022Quote: ... and the impurities that were tagged by Chitin Binding Domain were extracted on a gravity-flow column using 5 mL Chitin resin (New England BioLabs, Evry, France). The target proteins were purified with HisTrap HP columns (GE Life Sciences ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were prepared with NEBNEXT rRNA Depletion kit (human/mouse/rat) and NEBNEXT Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Derivatives of pEAI423 containing mutations in EXO1 were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAI423 as template and the oligonucleotides shown in Table S7 ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Immunology 2022Quote: ... was used to generate the CAR.PDZ with a PDZbm attached to the C-terminus of CD3ζ domain and site directed mutagenesis Q5(New England Biolabs, Ipswich, MA, USA) was used to do a point mutation to exchange the final Valine to an Alanine to create the CAR.PDZmut construct.