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Citations for New England Biolabs :
351 - 400 of 9480 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Constructs containing the alternate alleles were obtained by performing Q5 site-directed mutagenesis (NEB, #E0554S). Primers used to amplify targets and perform site-directed mutagenesis are listed in Table S9 ...
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bioRxiv - Cell Biology 2022Quote: ... The crude extracts were loaded into a column containing amylose resin (E8021S, New England Biolabs) for affinity chromatography ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Biochemistry 2019Quote: ... AP sites were generated by treating oligonucleotides containing an internal uracil with UDG (NEB, M0280S) (0.08U/μL reaction ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 μg of R-loop-containing plasmid was incubated with 0.5 U RNase H (NEB) in 75 mM KCl / 50 mM Tris-HCl pH 7.5 / 3 mM MgCl2 / 10 mM DTT in a 20 μL reaction volume and incubated at 37 °C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... and each aliquot was amplified in its own 50-μL reaction containing Phi29 polymerase (NEB) and exonuclease resistant random hexamers (Thermo ...
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bioRxiv - Microbiology 2022Quote: ... The beads were resuspended in 1X PBS containing NuPage LDS Sample Buffer (New England Biolabs) and dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... the allelic lacI-containing shuttle vector pMQ123 was first digested with BamHI (New England BioLabs) and the targeted small regulatory RNAs were amplified using Phusion polymerase (New England BioLabs ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... CNCCs were re-suspended in DEPC-PBS containing 0.04% non-acetylated BSA (New England Biolabs). Cell viability was determined to be >95% by Trypan Blue staining ...
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bioRxiv - Genomics 2023Quote: ... either NT of EMC2 guide containing inserts were T4 ligated (ensure it is NEB #M0202M) with an insert to vector ratio of 1:2 for a 16 hours at 16C ...
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bioRxiv - Cancer Biology 2023Quote: ... 30ng of genomic DNA was used for each reaction containing 7.5 U Φ29 (#M0269L, BioLabs), 1x Φ29 DNA polymerase buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Bioengineering 2024Quote: ... The resultant PCR product was reassembled into a tdTomato-containing vector via Gibson assembly (NEB) with a tdTomato insert (sequence ordered as a codon-optimized gBlock from IDT ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing Cdt1 were further purified on a 5 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Microbiology 2024Quote: ... the ExoI reaction mixture containing 1 x ExoI buffer and 1 U/μL ExoI (NEB) enzyme was prepared ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Cell Biology 2019Quote: ... We incubated cells in complete medium containing 10 μM of SNAP-Cell Block (New England Biolabs) to block all available pre-existing SNAP-tag (Quench) ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of PCR product containing barcoded oligos were inserted into 1 μg SfiI (NEB, R0123) digested empty vector A or P by gibson assembly NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... and then mounted using 10 μl ProLong Gold Antifade Reagent containing DAPI (9071S, New England Biolabs). The slides were imaged when the mounting medium was fully cured >12 h.
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bioRxiv - Evolutionary Biology 2020Quote: ... A DNA fragment containing the aanat2 target fragment was digested with BbvI (New England Biolabs Inc.). The rorca DNA fragment was digested with Cac8I (New England Biolabs) ...
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bioRxiv - Biochemistry 2019Quote: Activating adaptors containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at OD 0.4-0.6 with 0.1 mM IPTG for 16 hr at 18°C ...
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bioRxiv - Systems Biology 2019Quote: ... 5 mM CaCl2 and 25% (v/v) glycerol)) containing MNase ((micrococcal nuclease (M0247S, New England Biolabs)) and supplemented with protease inhibitor ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 mM CaCl2 and 1x PIC) containing 2U/μl of micrococcal nuclease (New England BioLabs, M0247S) and incubating on ice for 20 min and then 37°C for 10 min ...
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bioRxiv - Genomics 2019Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1_noMX universal primer ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Biochemistry 2020Quote: ... excised bands containing 20 μg of gp145 were incubated with 5000 units of PNGase F (NEB) for 24 hrs at 37°C [31] ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were quenched with native loading dye containing EDTA (New England Biolabs, Cat. no. #B7025S). Samples were loaded on a 0.7% agarose gel and run at 120 V in 45□mM (0.5× ...
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bioRxiv - Molecular Biology 2021Quote: ... The 20 µl reaction mix containing 80 ng of single-stranded M13mp18 DNA (New England Biolabs), 10 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μl of enzyme mixture containing 0.01 U Bst 2.0 DNA polymerase (New England Biolabs, M0537S), 0.5 U SplintR ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: Activating adaptors containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at OD 0.4-0.6 with 0.1 mM IPTG for 16 hr at 18°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Annealed oligonucleotides containing the homology region were phosphorylated with T4 polynucleotide kinase (New England Biolabs, M201) and then ligated into the Bbs1 site of the vectors pX335 and pKN7 (Appendix Tables S1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... with each containing five units of Taq Plus and polymerase buffer (New England, BioLabs®, UK), 2 mM MgCl2 ...
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bioRxiv - Pathology 2022Quote: ... and 1 mM DTT containing [3H] SAM (25:1 molar ratio of SAM(NEB, Cat#B9003S) to [methyl-3H]-SAM (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Developmental Biology 2019Quote: ... Crude cell lysate was passed through a Sepharose column containing 5mL amylose resin (New England Biolabs) and the MBP-tagged GFP-NT-Lysenin fusion protein was eluted with fresh 10 mM maltose in 1X PBS (pH 7.4) ...
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bioRxiv - Bioengineering 2019Quote: ... DNA fragments containing the AOX1 promoter and gene were amplified by PCR using Vent polymerase (NEB) with primer combinations AOX1P-F and AOX1P-R for the promoter and AOX1G-F and AOX1G-R for the gene (Table S3).
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bioRxiv - Developmental Biology 2020Quote: ... followed by permeabilization in PBS containing 0.5% Triton X-100 and ribonucleosidevanadyl complex (New England Biolabs) on ice for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 M guanidine HCl) containing 8 units/ml and Proteinase K (P8107S, New England Biolabs Inc.) was added freshly to the digestion buffer ...
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bioRxiv - Biochemistry 2021Quote: ... rinsed with PBS and incubated in PBS containing 3% molecular biology grade BSA (New England Biolabs) and 0.05% Tween-20 for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Phages containing the library were rescued using M13KO7 Helper Phage (New England Biolabs, Ipswich, MA, USA). Solution-phase bio-panning was conducted in order to minimize the disruption of a target protein ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Biophysics 2022Quote: ... containing TwinStrep-SUMO at the N-terminus of S15 using the NEBuilder HiFi assembly protocol (NEB). TnsC clones were transformed into Escherichia coli BL21(DE3 ...