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Citations for New England Biolabs :
551 - 600 of 10000+ citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’ ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) and ligated to a pre-adenylated 3’ adapter (L3-App) ...
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bioRxiv - Molecular Biology 2024Quote: ... and a 50 pm RNA probe was incubated with 20U T4 Polynucleotide kinase (PNK) (NEB, catalogue # M0201S), 1X T4 PNK buffer and 30 µCi P32ATP at 37°C for 30minutes (reaction volume 50µl ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Microbiology 2020Quote: ... Following KLD (kinase, ligase, and DpnI) treatment and transformation into NEB 5-alpha F’Iq cells (New England Biolabs), cells were grown as near-lawns on LB-chloramphenicol plates and a miniprep was performed from a resuspension of ~5000 colonies for each library.
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bioRxiv - Biochemistry 2020Quote: ... the top strand (MB733) was 5′ labelled with ϒ[32P]- ATP and T4 polynucleotide kinase (T4 PNK; NEB) for 1 h at 37°C and cleaned up with a G-50 micro column as above ...
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bioRxiv - Evolutionary Biology 2021Quote: 3’ dephosphorylation was performed by incubating fragments with 10 U/uL T4 Polynucleotide Kinase (New England Biolabs M0201S) in the supplied buffer (NEB B0201S ...
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bioRxiv - Systems Biology 2019Quote: ... De-phosphorylated RNA was purified with an RNeasy MinElute spin column and re-phosphorylated with Polynucleotide Kinase (NEB). PNK-treated tRNAs were purified with an RNeasy MinElute spin column and ...
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bioRxiv - Systems Biology 2019Quote: ... Each of the four-nucleic acid substrates were radiolabeled with [γ-32P]-ATP using T4 Polynucleotide Kinase (NEB). Free nucleotide was removed using G-25 MicroSpin columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... primers containing overhangs of the 3x FLAG peptide were phosphorylated using the T4 polynucleotide kinase (New England BioLabs). Phosphorylated primers were used to PCR amplify pQLinkN-pgaD to generate the plasmid pQLinkN-pgaD-FLAG ...
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bioRxiv - Neuroscience 2020Quote: ... The top and bottom oligos for the gRNA were phosphorylated and annealed using T4 polynucleotide kinase (PNK) (NEB) and then cloned into the pU6 vector (Addgene plasmid #53062) ...
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bioRxiv - Plant Biology 2021Quote: ... and subsequently end repair (T4 DNA polymerase, Klenow DNA polymerase and T4 Polynucleotide Kinase; New England Biolabs, USA) and A-tailing (Klenow Fragment ...
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bioRxiv - Microbiology 2020Quote: ... proteins were pre-treated with 0.5 mM ATP and 2.5 U casein kinase 2 (CK2) in 1X CK2 buffer (NEB) for 15 min at 30°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Non-overlapping forward and reverse primers containing the desired mutation were phosphorylated by polynucleotide kinase (New England Biolabs) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide pairs were resuspended to 100 μM and annealed in a polynucleotide kinase (PNK) mix (New England BioLabs) consisting of 1.5 μL of each oligonucleotide ...
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bioRxiv - Genomics 2019Quote: Pairs of complementary DNA oligos (IDTDNA, Coralville, IA) were individually phosphorylated with T4 polynucleotide kinase (NEB, Ipswitch, MA) and then annealed ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Genomics 2021Quote: ... in a 25 μL reaction containing 1X PNK buffer and 15 units of polynucleotide kinase (New England Biolabs) by incubating at 37°C for 45 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... gRNA oligos were annealed using T4 Polynucleotide Kinase (Fisher# 50811602) in T4 ligation buffer (New England Biolabs# B0202S) and then inserted into pU6-2-gRNA plasmids using T4 DNA ligase (New England Biolabs # M0202S).
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM DTT) and the 5’-ends were phosphorylated with 25 units of T4 Polynucleotide Kinase (NEB #M0201) for 15 min at 37 °C while shaking in a thermomixer at 1000 rpm for an interval of 15 sec every 3 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... Each oligonucleotide pair was phosphorylated and annealed using the T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA, USA). Duplexed oligonucleotides were ligated into BbsI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: Pre-crRNA was 5’-radiolabeled with [γ−32P] ATP (Perkin-Elmer) using T4 polynucleotide kinase (New England Biolabs). For some measurements ...
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bioRxiv - Biochemistry 2023Quote: ... 13 pmol of purified RNA was phosphorylated with y-32P ATP (Hartmann Analytics) by T4 polynucleotide kinase (NEB) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by addition of a phosphate group to the 5’ end using T4 polynucleotide kinase (T4 PNK, NEB). Next ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of the obtained RNA were then radiolabeled by incubation with 1 U of Polynucleotide Kinase (NEB) and 20 μCi of 32P-γATP for 1 h at 37°C in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2023Quote: ... 50pmol of each oligo were mixed in a 25µL reaction and phosphorylated with T4 polynucleotide kinase (M0201S, NEB). Reactions were performed for 30 min at 37°C in 1X T4 DNA ligase buffer (B0202S ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides corresponding to the ‘right’ half were 5′-phosphorylated using T4 polynucleotide kinase (New England Biolabs, Ipswitch, MA). Equimolar amounts of ‘right’ strand ...
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bioRxiv - Biochemistry 2023Quote: ... and a final concentration of 1.4 nM of hRNase 4 and 0.15 U/μL of polynucleotide kinase (New England Biolabs). The reactions were incubated at 37°C for 1 h and stopped by addition of murine RNase inhibitor to a final concentration of 2 U/μL and incubation at room temperature for 10 minutes ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... oligonucleotides encoding the crRNA sequences were custom synthesized (Integrated DNA Technologies) and incubated with T4 polynucleotide kinase (NEB) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Specific probes were generated by end-labeling of oligodeoxyribonucleotides (Table S3) using T4 Polynucleotide Kinase (New England Biolabs) and [γ-32P]-ATP (Hartmann Analytic) ...
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bioRxiv - Microbiology 2023Quote: ... plasmid was pre-treated with Alkaline phosphatase and amplicons were treated with T4 polynucleotide kinase (New England Biolabs). Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... oligonucleotides encoding the crRNA targeting sequences were synthesized (Integrated DNA Technologies) and incubated with T4 polynucleotide kinase (NEB) for 30 min at 37° ...
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bioRxiv - Neuroscience 2024Quote: ... The fragments were re-phosphorylated at their 5’-hydroxyl groups using T4 polynucleotide kinase (New England Biolabs; M0201S) and purified using the miRNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Oligos containing the spacer sequence and Esp3I sticky ends were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) and purified using the Oligo Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... sgRNA designed by online design tool (http://chopchop.cbu.uib.no/) and two DNA oligo annealing by T4 Polynucleotide Kinase (NEB, Cat#M0201L) and cloned into a Bbs1-digested U6-sgRNA-PGK-Puro-BFP retrovirus vector by T4 DNA ligation ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... Each RNA was subjected to radioactive end-labeling or non-radioactive phosphorylation by T4 Polynucleotide Kinase (New England Biolabs). Each RNA was subjected to phosphorylation with equimolar concentrations of either 32P-γ-ATP or ATP ...
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bioRxiv - Microbiology 2021Quote: ... a 39-mer oligonucleotide complementary to the spliced leader was labelled with [γ32P]-ATP using T4 polynucleotide kinase (NEB) and incubated with the membrane overnight at 42°C ...
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bioRxiv - Cell Biology 2022Quote: ... to a final concentration of 0.5 μCi/μL and 0.8 U/μL T4 polynucleotide kinase (New England BioLabs, M0201S) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... custom synthesized oligonucleotides encoding the sgRNA spacer sequences (IDT) (Supplementary Table S1) were phosphorylated by T4 polynucleotide kinase (NEB) for 30 min at 37°C and subsequently annealed in a thermocycler (5 min at 95° ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’end of RNA was phosphorylated and labelled by γ-[32P] ATP using T4 Polynucleotide Kinase (New England Biolabs) at 37°C for 45 min ...
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bioRxiv - Biochemistry 2020Quote: Every single stranded oligonucleotide (1 nmol) was 5’-end-phosphorylated with 40U of T4 Polynucleotide kinase (New England Biolabs) by incubation at 37°C in 1x T4 DNA Ligase Reaction Buffer.
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotides complimentary to the target were end-labeled with γ-32P-ATP by T4 polynucleotide kinase (New England Biolabs). Labeled oligonucleotides were added to the membrane and incubated overnight at 45°C ...
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bioRxiv - Biochemistry 2022Quote: ... End repair of RPFs RNA samples was conducted through dephosphorylation reaction—by adding 7.5 μL T4 polynucleotide kinase (PNK) 10X buffer (B0201S, NEB), 1.5 μL ATP (100 mM ...
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bioRxiv - Microbiology 2020Quote: ... and the 5’ ends of the DNA (25 nM) were radiolabeled using T4 polynucleotide kinase (PNK, New England Biolabs). Excess ...
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bioRxiv - Immunology 2021Quote: ... Complimentary oligonucleotides encoding the sgRNAs with suitable overhangs for ligation were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) with the T4 DNA Ligase Reaction buffer (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... DNA oligonucleotides (Supplementary Table 2) were radiolabeled at the 5’ end by T4 Polynucleotide kinase (PNK) (New England Biolabs) treatment and [γ-32P] (PerkinElmer ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...