Labshake search
Citations for New England Biolabs :
451 - 500 of 10000+ citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were annealed and phosphorylated with 5U of T4 Polynucleotide Kinase (New England Biolabs). Methylation constructs were obtained with the Golden Gate assembly cloning strategy based on BbsI restriction enzyme ...
-
bioRxiv - Plant Biology 2024Quote: ... The RNA was ethanol precipitated and phosphorylated with T4 polynucleotide kinase (New England Biolabs) prior to library construction using the NEXTFLEX Small RNA-Seq Kit v4 (Perkin Elmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... the plasmids were phosphorylated by T4 Polynucleotide Kinase and ligated by T4 Ligase (NEB).
-
bioRxiv - Molecular Biology 2024Quote: ... Extracted RNA fragments were first treated with T4 Polynucleotide Kinase (T4 PNK, NEB, M0201L) without adding ATP to remove the 3’ end phosphates ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 5′ end labelled using γ32P UTP (Perking Elmer) and T4 polynucleotide kinase (NEB) following manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
-
bioRxiv - Microbiology 2024Quote: ... 50 μg RNA was treated with T4 polynucleotide kinase (New England Biolabs, MA, USA) according to the manufacturer’s instructions at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... or beads alone were suspended in 1x NEBuffer™ for Protein Kinases with 200μM ATP (Thermo) and 100 units of CK2 (NEB) at 30°C for 45 minutes ...
-
bioRxiv - Microbiology 2021Quote: Protein bound glutathione Sepharose beads were resuspended in 1x NEBuffer™ for Protein Kinases supplemented with 1mM ATPγS and 10 units of CK2 (NEB) and incubated at 30°C for 45 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA-protein complexes were radioactively labeled with 32P-γ-ATP (20 μCi) using 40U T4 Polynucleotide Kinase (New England Biolabs) in supplied PNK buffer for 30 min at 37ºC ...
-
bioRxiv - Biochemistry 2019Quote: ... GST fusion proteins of the CTD and its mutants CTD-AA and CTD-YA were phosphorylated by Cdc2 kinase (NEB) using 32P-γ-ATP for 30 minutes at 30°C before incubated with immunoprecipitated CoAA or hnRNP M ...
-
bioRxiv - Cancer Biology 2023Quote: Kinase-dead mutant (K37R) of Nek2A was derived from Nek2A in pJP1563 using Q5 Site-Directed Mutagenesis Kit (NEB) following the instructions of manufacturer ...
-
bioRxiv - Developmental Biology 2021Quote: ... Top and bottom guide RNAs were annealed with T4 Polynucleotide Kinase (3’ phosphatase minus, NEB) and T4 DNA Ligase Buffer (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides were 5-end labeled with 32P-γATP using T4 polynucleotide kinase (New England Biolabs). Supercoiled pUC19 plasmid dsDNA was prepared by a method that did not involve DNA denaturation (Clewell and Helinski ...
-
bioRxiv - Biochemistry 2020Quote: 100 ng of purified tsRNAs were treated with 0.5 U/µL T4 polynucleotide kinase (NEB) and 166 µM ATP (NEB ...
-
bioRxiv - Synthetic Biology 2020Quote: ... if necessary (crRNA arrays) also phosphorylated with T4 polynucleotide kinase (New England Biolabs, MA, USA) and ligated with T4 DNA ligase in previously BsmbI digested vectors.
-
bioRxiv - Genomics 2021Quote: ... PCR fragments were labeled using 32P-γ-ATP with T4 polynucleotide kinase (New England, Biolabs). Protein–DNA complexes were incubated for 1h at room temperature in binding buffer containing 25 mM Tris pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... which was purchased from Integrated DNA Technologies and end-labeled with T4 polynucleotide kinase (NEB) and γP32-ATP (6000 Ci/mmol ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
-
bioRxiv - Biochemistry 2020Quote: ... and subsequently 5’32P-labeled using [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs) according to the manufacturer’s protocols.
-
bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
-
bioRxiv - Molecular Biology 2022Quote: ... size selected ribosome footprints were treated with T4 Polynucleotide Kinase (#M0201L, New England Biolabs (NEB)) followed by ligation to a DNA linker using T4 RNA ligase 2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... PLPs were ordered as large pools and phosphorylated with T4 Polynucleotide Kinase (New England Biolabs). Sequences of all oligonucleotides can be found in Supplementary Table 1-4 ...
-
bioRxiv - Microbiology 2021Quote: ... primers MR161 and MR162 (Table 2) were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs) at 37°C for 1 h in a 4.5 μl reaction volume with 10 μM primer ...
-
bioRxiv - Cancer Biology 2022Quote: ... Phosphorylation of the 5’ end using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA, M0201L) (iii ...
-
Phospho-sRNA-seq reveals extracellular mRNA/lncRNA fragments as potential biomarkers in human plasmabioRxiv - Molecular Biology 2019Quote: ... synthetic and plasma RNA samples were pretreated with T4 polynucleotide kinase (NEB, Ipswich, MA, US) using an RNA input of 7 ul in a final reaction volume of 10 ul and following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Biochemistry 2021Quote: ... oligonucleotide annealing was carried out in a thermocycler in T4 polynucleotide kinase reaction buffer (NEB) / 50 mM potassium acetate by heating oligonucleotide mixtures to 95 °C for 5 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ends of the fragmented RNA were repaired by treatment with T4 Polynucleotide Kinase (NEB) and Calf intestinal alkaline phosphatase (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... gRNA was phosphorylated by a T4 polynucleotide kinase according to product instructions (New England Biolabs). For the amplification of phosphorylated sgRNA it was incubated with 1 μl of forward and reverse primers (100 μM ...
-
bioRxiv - Biophysics 2023Quote: ... the insert PCR fragment was phosphorylated with P4 polynucleotide Kinase (M0201, New England Biolabs, UK) and the destination PCR-fragment was DPN1 treated (R0176 ...
-
bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was started by adding 5000 units of recombinant Casein Kinase II (NEB, P6010S) and left to incubate for 20 min at 30°C with gentle agitation ...
-
bioRxiv - Cell Biology 2023Quote: ... Sense and antisense oligos were annealed with T4 Polynucleotide Kinase (PNK) (New England Biolabs; #M0201) and ligated with the digested cutting plasmid with T4 DNA ligase (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2023Quote: ... To prepare the TEV substrate two oligos were annealed using T4 Polynucleotide Kinase (NEB#M0201S) and T4 DNA ligase buffer (NEB#B0202S) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were 3’ dephosphorylated and 5’ phosphorylated with T4 polynucleotide kinase (T4PNK, NEB, #M0201S), and purified with RNA Clean and Concentrator-5 (Zymo Research ...
-
bioRxiv - Microbiology 2023Quote: ... The 5’ ends were radiolabelled with 20 μCi γ32P-ATP using T4 polynucleotide kinase (NEB) and separated from free nucleotides using a MicroSpin G-50 column (Cytiva) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the complementary oligos were phosphorylated using T4 polynucleotide kinase from New England Biolabs (NEB, USA) first and then ligated to pVT36b using Golden Gate Assembly (Engler et al. ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified fragments were dephosphorylated at their 3′ ends with T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q ...
-
bioRxiv - Molecular Biology 2023Quote: ... Probes were synthesized as ssDNA oligos and 5’ 32P-radiolabeled with T4 polynucleotide-kinase (NEB) and ψ-32P-ATP (6000 Ci/mmol ...
-
Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Seventy picomoles of oligonucleotide (40T) was labelled with polynucleotide kinase (PNK) in PNK buffer (NEB) in the presence of γ32P-ATP (Perkin-Elmer ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 ng of GLORI-treated RNA sample was repaired using T4 Polynucleotide Kinase (NEB, #M0201S). Pre-adenylated 3’-adapter and 5’-adapter were sequentially ligated to the RNA using T4 RNA Ligase 2 ...
-
bioRxiv - Biophysics 2023Quote: ... each primer pair was annealed and consequently phosphorylated with T4-polynucleotide kinase (T4-PNK; NEB). Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo ...
-
bioRxiv - Plant Biology 2024Quote: ... dephosphorylated F4 (5 µM) was incubated with γ-[32P]-ATP and T4 polynucleotide kinase (NEB) for 45 min at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
-
bioRxiv - Neuroscience 2022Quote: ... The non-15N 4R tau used for small-scale chemical in vitro crosslinking was phosphorylated using cAMP-dependent Protein Kinase (PKA, catalytic subunit, New England Biolabs #P6000S) according to manufacturer guidelines (25uL reaction at 30°C for 2 hours with 200 μM ATP ...
-
bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biophysics 2023Quote: Tau was phosphorylated in vitro according to previous protocols with some modifications.12,88 50 µL of 100 µM tau was incubated with 2 µg cAMP-dependent Protein Kinase (PKA, New England BioLabs P6000S) and/or 0.4 µg GSK3β (Sigma-Aldrich G4296 ...
-
bioRxiv - Cell Biology 2020Quote: ... The kinase-inactive mutant was generated by a two-step PCR method using the Phusion High-fidelity kit (New England Biolabs). In the first step ...