Labshake search
Citations for New England Biolabs :
401 - 450 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: In vitro translation was monitored by the production of luciferase signal in a PURExpress in vitro protein synthesis kit (NEB), using firefly luciferase mRNA as an input ...
-
bioRxiv - Immunology 2021Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector containing the constant part of IgG1 heavy or kappa/lambda light chain sequences respectively were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Gene-specific primers were used on cDNA (OneTaq One-Step RT-PCR, New England Biolabs) to evaluate aberrant transcript splicing via gel electrophoresis ...
-
bioRxiv - Microbiology 2021Quote: ... The digested pGRG36 and cloned chitinase gene were ligated together using ElectroLigase (New England Biolabs) and used to transform One Shot Electrocompetent cells ...
-
bioRxiv - Genomics 2020Quote: KlenTaq wild type gene was amplified using a high fidelity PCR (Q5 polymerase from NEB) from a stored plasmid ...
-
Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... The bar gene was amplified with Phusion ® High-Fidelity DNA polymerase (NEB cat# M0530) from the pAL156 vector (Amoah et al. ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNA of indicated genes were amplified by PCR using Q5 HF DNA polymerase (NEB, #M0491L) and the following specific primers ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The spiralen promoter and mCherry gene were amplified using Q5 master mix (New England Biolabs), with the plasmid pTF20mChloxp (gift from Kevin Dybvig ...
-
bioRxiv - Plant Biology 2021Quote: ... the gene flanking regions were fused to an antibiotic resistance cassete using NEB builder (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
-
bioRxiv - Biochemistry 2021Quote: ... Conditional gene deletion was assessed by PCR amplification of genomic DNA using Taq polymerase (NEB) and oligonucleotides SMD157/160 flanking the TbGT10 locus ...
-
bioRxiv - Biophysics 2020Quote: ... tinctorius Nav1.4 genes by polymerase chain reaction (PCR) using Phusion® HF (New England Biolabs). PCR products were gel extracted and sequenced to determine the full-length P ...
-
bioRxiv - Microbiology 2021Quote: The genes ApiAP2 (PF3D7_0934400) and rifin (PF3D7_1254400) were PCR amplified using Q5 DNA polymerase (NEB) from P ...
-
bioRxiv - Biochemistry 2022Quote: ... Purified genes were restriction enzyme-digested and ligated into pUC19 vectors (New England Biolabs, NEB). Iterative rounds of digestion and ligation were used to produce each successively longer recA oligomer construct ...
-
bioRxiv - Biochemistry 2022Quote: ... Purified genes were restriction enzyme-digested and ligated into pUC19 vectors (New England Biolabs, NEB). Iterative rounds of digestion and ligation were used to produce each successively longer recA oligomer construct ...
-
Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... PCR amplification of genes of interest for this study was done with Phusion polymerase (NEB). For pET vector cloning the PCR amplicons were digested with restriction endonucleases NdeI/XhoI for pET29b/pETduet-1 MCS2 or BamHI/SalI for pETduet-1 MCS1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 50 μL PCR reactions for each gene block were carried out with Phusion polymerase (NEB) using primers gblock_fwd and gblock_rev for 35 cycles (denaturing ...
-
bioRxiv - Biochemistry 2023Quote: ... Target genes were then cloned into pCellFree_03 using the Gibson assembly protocol (New England Biolabs). Nup98 and Pom121 fragments were purchased as gBlocks from IDT for cloning into cloned into pCellFree_03 ...
-
bioRxiv - Immunology 2023Quote: ... The gene insertion into the vector was confirmed by using restriction digestion by SgrA1 (NEB) and plasmid sequencing (Azenta LifeSciences) ...
-
bioRxiv - Microbiology 2023Quote: Turicibacter genes were amplified from template culture lysates with Phusion or Q5 DNA polymerase (NEB) and primers designed to amplify denoted Turicibacter genes ...
-
bioRxiv - Microbiology 2023Quote: ... gene specific oligonucleotides (S4 Table) and a high-fidelity DNA polymerase (Phusion; New England BioLabs). Amplified gene fragments were inserted via digestion/ligation cloning into (Isopropyl-β-D-1-thiogalactopyranoside ...
-
bioRxiv - Molecular Biology 2024Quote: ... Conditional gene deletion was assessed by PCR amplification of genomic DNA using Taq polymerase (NEB) and oligonucleotides SMD357/8 flanking the TbGT8 locus ...
-
bioRxiv - Microbiology 2024Quote: ... for cellular genes or the Luna universal probe qPCR Master Mix (New England Biolabs, M3004) for the viral transcripts ...
-
bioRxiv - Microbiology 2024Quote: ... Each gene was PCR amplified from the plasmids using Q5 polymerase (New England Biolabs; NEB) with the primers 5’-GAAGTGCCATTCCGCCTGACC and 5’-CACTGAGCCTCCACCTAGCC ...
-
bioRxiv - Microbiology 2024Quote: ... Each gene was PCR amplified from the plasmids using Q5 polymerase (New England Biolabs; NEB) with the primers 5’-GAAGTGCCATTCCGCCTGACC and 5’-CACTGAGCCTCCACCTAGCC ...
-
bioRxiv - Microbiology 2024Quote: ... Deletions of single genes in plasmid pGM255 were performed using the KLD enzyme mix (NEB).
-
bioRxiv - Microbiology 2024Quote: ... PCR products and the respective antibiotic resistance gene were used for Gibson assembly (NEB, USA) (38) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
-
bioRxiv - Neuroscience 2021Quote: ... Lambda Protein Phosphatase (NEB) assay was performed as described by the supplier ...
-
bioRxiv - Biochemistry 2020Quote: ... Cas9 protein (NEB M0641S) and phenol red injection dye ...
-
bioRxiv - Developmental Biology 2021Quote: Cas9 protein (M0646, NEB) and sgRNAs against antisense of exons 14 ...
-
bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
-
bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Immunology 2024Quote: N-linked glycans were enzymatically released from purified mouse THP using PNGase-F kit (catalog no P0709S, New England Biolabs). N-glycans were then purified from the reaction mixture containing denaturing buffer and de-N-glycosylated proteins by solid phase extraction method using Sep-Pak C18 (1 cc Vac-cartridges ...
-
bioRxiv - Microbiology 2024Quote: ... AnTat1.1 sequence was obtained from AnTat1.1 specific cDNA from mouse infection D6 cloned into a pMiniT vector with the PCR Cloning Kit (NEB, E1202S). VSG-228 was partially amplified from VSG PCR (see below ...
-
bioRxiv - Plant Biology 2021Quote: Purified LPR1 protein was analyzed using Protein Deglycosylation Mix II (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein (200 µg) was precleared with protein G magnetic beads (New England Biolabs) and incubated overnight (4° C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cleaved G protein was dephosphorylated by lambda protein phosphatase (NEB, Frankfurt, Germany), calf intestinal phosphatase (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... The protein ladder used was Colour Protein Standard Broad Range (NEB, Ipswich, US).
-
bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: NSD1/2 WT and different cancer mutants (3.4 μM) were mixed with recombinant H3.1 protein (1 μg) (purchased from NEB) or recombinant H3.1 mononucleosomes in methylation buffer (50 mM Tris/HCl ...
-
bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
-
bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...
-
bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 Spike mutant D614G was generated by site-directed mutagenesis using as an input DNA the expression vector encoding SARS-CoV-2 Spike_614D protein (kindly provided by J. Garcia-Arriaza, CNB-CSIC) by Q5 Site Directed Mutagenesis Kit (New England Biolabs, Barcelona, Spain) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
-
bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
-
bioRxiv - Microbiology 2019Quote: ... 500nM gene specific or 65nM 16S rRNA primer and 0.5 units Hot Start Taq Polymerase (NEB), and 10ng of template cDNA ...