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Citations for New England Biolabs :
51 - 100 of 8932 citations for Medium Kit without Serum and without Growth Factors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA preparations were first 3’-dephosphorylated using T4 PNK for 1h at 37°C without ATP and pre-adenylated linker (Universal miRNA cloning linker, NEB) ligation was performed during 4h at 22°C in the presence of truncated RNA ligase 2 (NEB)30 ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The reactions were combined and 2.5 μL of the assembly reaction or a control reaction without amplicon were used to transform NEB5-alpha cells (New England Biolabs) to measure background assembly ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
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bioRxiv - Genetics 2019Quote: ... DNA samples were incubated at 20°C for 4h without rotation in a mix of 10 µl of 10x NEB2 buffer (New England Biolabs), 1 mM of a dNTPs mix (10 µl) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and assembling with the cpGFP fragment (with or without LSSmOrange) into pET19b between BlpI and NcoI using Gibson assembly (NEB). All primers are listed in supplementary table 3 ...
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bioRxiv - Cell Biology 2021Quote: ... The β1 Integrin-HaloTag-GFP without ARL13B–C terminus (I-GFP) was generated by Q5 site directed mutagenesis (New England Biolabs).
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bioRxiv - Immunology 2020Quote: For recombinant CARD proteins, NLRP1 constructs (985-1473, 1213-1473, 1374-1466, 1374–1473) with or without C-terminal SNAP tag (NEB), CARD8–CARD (451-537 ...
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bioRxiv - Molecular Biology 2021Quote: ... Single sample without barcodes or pooled barcoded samples (700 ng) were ligated to an ONT Adapter with NEBNext Quick Ligation Module (NEB), followed by purification with DNA Clean Beads ...
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bioRxiv - Molecular Biology 2022Quote: Purified modified RNA and RNA without any chemical modification were digested with the Nucleoside digestion mix (M0649, New England Biolabs) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... which includes the genomic sequence beginning at the end of the gene just upstream of SIC to the end of the SIC coding sequence (without the stop codon) was made with Q5 High-Fidelity DNA Polymerase (New England Biolabs) in three steps ...
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bioRxiv - Cell Biology 2023Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without 1 U Endo H (NEB, Frankfurt, Germany). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Molecular Biology 2023Quote: ... Coli vectors expressing SAHS proteins without SUMO tags were ordered and synthesized from Twist Bioscience and transformed into LEMO21(DE3) competent cells (NEB). To test for the effects of intracellular SAHS expression ...
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bioRxiv - Biochemistry 2023Quote: ... crosslinking of Pex1-AzF was performed after a 10 min 30 deg C incubation in ATP regeneration mix with and without apyrase (NEB#M0398S ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were washed three times with 1× PBS and incubated with or without 60 U/mL RNase H (M0297S, NEB) at 37°C for 36 h or left untreated ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 200 µM ATP with ot without 1 µl of recombinant PKA catalytic subunit (PKAc; New England Biolabs) in manufacturer-supplied reaction buffer at 30° C for 30min with agitation ...
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bioRxiv - Genomics 2021Quote: ... 2 μg genomic DNA from mESCs (both, with DSB induction and control without the induction) were digested using 1 μl 100 U/μL XbaI (NEB, #R0145T) and 4 μl 5 U/μL I-SceI (NEB ...
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bioRxiv - Biophysics 2020Quote: The cell-free transcription/translation mix used in this study is a customized version of the PURE system (40) without ribosomes (PUREexpress Δ ribosomes, New England Biolabs) and the amino acid histidine ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 24 h bacterial culture in LB without NaCl was isolated from bacteria using TRIzol reagent (HiMedia) and cDNA synthesized using ProtoScript® II Reverse Transcriptase (NEB). qRT-PCR was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then divided into two aliquots and treated with and without 100 U M.CviPI GpC methyltransferase (100 U/million cells; New England Biolabs, M0227B-HI) supplemented with fresh 160 μM S-adenosyl-L-methionine for 15 min at 37ºC ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Genomics 2022Quote: ... In vitro methylation of the plasmid with and without the SOCS3 insert was carried out using M.SssI methylase enzyme (NEB, cat.no: M0226S) following standard protocols and successful methylation of the insert was confirmed by digestion with methylation sensitive restriction enzymes AfeI ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 150-ng DNA from each sample with or without 2 units of Phi29 polymerase (New England Biolabs, Cat# M0269L) in 20-μl total reaction volume and incubated at 30 °C for 8 hours ...
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bioRxiv - Bioengineering 2020Quote: ... The modification of mmACP and mtDod-mmACP (both variants with H8-Tag and without) with CoA 488 (CoA modified with ATTO-TEC dye ATTO 488, NEB #S9348) by Sfp was conducted in phosphate borate buffer (pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... The ICabs were sub-cloned in pET22b vector without pelB signal sequence and over-expressed in SHuffle T7 cells of E.coli (NEB, catalogue no. C3026J).
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed 2 times with EB2 without NaF or PhosStop and one time with λpp buffer (New England Biolabs, Ipswich, MA) before resuspension in 40 μl of λpp buffer ...
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bioRxiv - Genomics 2019Quote: ... Glucosylated DNA was then end repaired without purification followed by ligation to Pyrrolo-dC adaptors as indicated in NEBNext Ultra II DNA library preparation protocol (NEB, Ipswich, MA, USA) with the following modification to the protocol ...
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bioRxiv - Microbiology 2021Quote: ... The digested DNA (4 ml in total) was split into 4 aliquots and diluted in 8 ml ligation buffer (1X ligation buffer NEB 3 (without ATP), 1 mM ATP ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA (400 ng) was either mock digested (i.e. without enzyme addition) or digested with BglII (New England Biolabs, 5 units/rxn, NEBuffer 3.1) for 1 hour at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Microbiology 2019Quote: ... Growth was monitored every 2 min using a Cell Growth Quantifier (Aquila Biolabs).
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Plant Biology 2021Quote: ... Factor Xa (P8010S, New England BioLabs) or TEV protease (P8112S ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated at 37ºC in complete growth medium containing either 50 nM SNAP-tetramethylrhodamine (TMR) star (New England BioLabs: S9105S) for 60 min ...
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bioRxiv - Systems Biology 2019Quote: ... The growth was continuously monitored online using Cell Growth Quantifier (CGQ) (Aquila Biolabs GmBH), and the samples were withdrawn at the start of the experiment ...
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bioRxiv - Systems Biology 2019Quote: ... 30°C and the growth was continuously monitored online using Cell Growth Quantifier (Aquila Biolabs GmBH). Samples were withdrawn at regular intervals for analyzing the spent metabolites through High-Performance Liquid Chromatography (HPLC).
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bioRxiv - Cell Biology 2021Quote: All dox-inducible factors were generated by cloning the open reading frame of each factor into the pMINI vector (NEB) and then restricted with EcoRI or MfeI and inserted into the FUW-TetO expression vector ...
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bioRxiv - Biochemistry 2022Quote: In vitro protein translation was carried out (in absence of the release factors) using the PURExpress ΔRF123 kit (New England Biolabs). This guaranteed the stabilization of the ternary complexes mRNA-affitin-Ribosome ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
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bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Molecular Biology 2023Quote: ... and amplified by lytic growth in LE392 cells (NEB). Following lytic growth ...
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bioRxiv - Biochemistry 2021Quote: ... MBP was cleaved from LH using Factor Xa (New England Biolabs) at a w/w ratio of 1% Factor Xa:LH ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with PBS and the cells were incubated in 1ml serum free medium and 250U/ml PNGase F (NEB, P0704S) for 6h ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were monitored using the Cell Growth Quantifier (Aquila Biolabs) with readings every 60 s and incubated at 37 °C and 210 rpm until the late stationary phase was reached ...